Recombinant expression and purification of Ssa1p (Hsp70) from Saccharomyces cerevisiae using Pichia pastoris

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 786, Issue 1-2, 2003, Pages 109-115

  Wegele, Harald ^a   Haslbeck, Martin ^a   Buchner, Johannes ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

ATPase; Hsp70; Purification; Ssa1p

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; GENES; PURIFICATION;

HEAT SHOCK PROTEINS;

PROTEINS;

ADENOSINE TRIPHOSPHATE; AGAROSE; HEAT SHOCK PROTEIN 70; RECOMBINANT PROTEIN;

CHROMATOGRAPHY; CONFERENCE PAPER; CONTROLLED STUDY; CYTOSOL; ESCHERICHIA COLI; FUNGAL GENE; GENE SEQUENCE; GENOME; INTRACELLULAR SPACE; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE;

PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037465664     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-0232(02)00724-9     Document Type: Conference Paper

References (32)

1. Gething M.J., Sambrook J. Nature. 355:1992;33.
2. Craig E.A., Weissman J.S., Horwich A.L. Cell. 78:1994;365.
3. Flaherty K.M., DeLuca-Flaherty C., McKay D.B. Nature. 346:1990;623.
4. Zhu X., Zhao X., Burkholder W.F., Gragerov A., Ogata C.M., Gottesman M.E., Hendrickson W.A. Science. 272:1996;1606.
5. Morimoto R., Tissieres A., Georgopoulus C. The Biology of Heat Shock Proteins and Molecular Chaperones. Cold Spring Harbor Monograph Series. 1994;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
6. Hartl F.U. Nature. 381:1996;571.
7. Werner-Washburne M., Stone D.E., Craig E.A. Mol. Cell. Biol. 7:1987;2568.
8. Craig E., Boorstein W., Park H.-O., Stone D., Nicolet C.M. UCLA Symp. Mol. Cell. Biol. 96:1989;51.
9. Palleros D.R., Reid K.L., Shi L., Welch W.J., Fink A.L. Nature. 365:1993;664.
10. Banecki B., Zylicz M. J. Biol. Chem. 271:1996;6137.
11. Schmid D., Baici A., Gehring H., Christen P. Science. 263:1994;971.
12. Greene L.E., Zinner R., Naficy S., Eisenberg E. J. Biol. Chem. 270:1995;2967.
13. McCarty J.S., Buchberger A., Reinstein J., Bukau B. J. Mol. Biol. 249:1995;126.
14. Lopez-Buesa P., Pfund C., Craig E.A. Proc. Natl. Acad. Sci. USA. 95:1998;15253.
15. Liberek K., Marszalek J., Ang D., Georgopoulos C., Zylicz M. Proc. Natl. Acad. Sci. USA. 88:1991;2874.
16. Cyr D.M., Lu X., Douglas M.G. J. Biol. Chem. 267:1992;20927.
17. Lu Z., Cyr D.M. J. Biol. Chem. 273:1998;27824.
18. Cyr D.M., Douglas M.G. J. Biol. Chem. 269:1994;9798.
19. Cregg J.M., Vedvick T.S., Raschke W.C. Bio/Technology. 11:1993;905.
20. Grinna L.S., Tschopp J.F. Yeast. 5:1989;107.
21. Ellis S.B., Brust P.F., Koutz P.J., Waters A.F., Harpold M.M., Gingeras T.R. Mol. Cell. Biol. 5:1985;1111.
22. Cregg J.M., Madden K.R., Barringer K.J., Thill G.P., Stillman C.A. Mol. Cell. Biol. 9:1989;1316.
23. Adams A., Gottschling D.E., Kaiser C.A., Staerns T. Methods in yeast genetics. A Cold Spring Harbor Laboratory Manual. 1997;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Fling S.P., Gregerson D.S. Anal. Biochem. 155:1986;83.
25. Heukeshoven J., Dernick R. Electrophoresis. 9:1988;28.
26. Fairbanks G., Steck T.L., Wallach D.F.H. Biochemistry. 10:1971;2606.
27. Schäfer H., Nau K., Sickmann A., Erdmann R., Meyer H.E. Electrophoresis. 22:2001;2955.
28. Kornberg A., Scott J.F., Bertsch L.L. J. Biol. Chem. 253:1978;3298.
29. Cregg J.M., Cereghino J.L., Shi J., Higgins D.R. Mol. Biotechnol. 16:2000;23.
30. Higgins D.R., Cregg J.M. Methods Mol. Biol. 103:1998;1.
31. Gao B., Greene L., Eisenberg E. Biochemistry. 33:1994;2048.
32. Theyssen H., Schuster H.P., Packschies L., Bukau B., Reinstein J. J. Mol. Biol. 263:1996;657.