Four different clones of mouse anti-acetyllysine monoclonal antibodies having different recognition properties share a common immunoglobulin framework structure

Journal of Immunological Methods

Volumn 272, Issue 1-2, 2003, Pages 161-175

  Komatsu, Yasuhiko ^a,b,c   Yukutake, Yoshinori ^a,c   Yoshida, Minoru ^a,d  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b JAPAN ENERGY CORPORATION   (Japan)

^c KEIO UNIVERSITY   (Japan)

^d RIKEN   (Japan)

Author keywords

DNA sequence; ELISA; Monoclonal antibody; N acetyllysine; Western blotting

Indexed keywords

ACETYLLYSINE ANTIBODY; AMINO ACID; COMPLEMENTARY DNA; GLYCYL N EPSILON ACETYLLYSYL EPSILON AMINOCAPROIC ACID CYSTEINE; LYSINE DERIVATIVE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY AKL3H6; MONOCLONAL ANTIBODY AKL5C1; MONOCLONAL ANTIBODY AL11; MONOCLONAL ANTIBODY AL3D5; N EPSILON ACETYLLYSINE; PEPTIDE; UNCLASSIFIED DRUG;

ACETYLATION; ANALYTIC METHOD; ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY SCREENING; ANTIGEN DETECTION; ANTIGEN RECOGNITION; ARTICLE; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; FEMALE; HYBRIDOMA; IMMUNIZATION; IMMUNOGLOBULIN STRUCTURE; IMMUNOREACTIVITY; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; WESTERN BLOTTING;

ACETYLATION; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BASE SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; COMPLEMENTARITY DETERMINING REGIONS; DNA, COMPLEMENTARY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HYBRIDOMAS; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; LYSINE; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; PRECIPITIN TESTS; PROTEINS; SEQUENCE HOMOLOGY, NUCLEIC ACID;

BOVINAE;

EID: 0037438143     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00500-8     Document Type: Article

References (26)

1. Akolkar P.N., Sikder S.K., Bhattacharya S.B., Liao J., Gruezo F., Morrison S.L., Kabat E.A. Different VL and VH germ-line genes are used to produce similar combining sites with specificity for α(1→6)dextrans. J. Immunol. 138:1987;4472-4479.
2. Christenson L.K., Stouffer R.L., Strauss J.F. III Quantitative analysis of the hormone-induced hyperacetylation of histone H3 associated with the steroidogenic acute regulatory protein gene promoter. J. Biol. Chem. 276:2001;27392-27399.
3. Eberhardy S.R., D'Cunha C.A., Farnham P.J. Direct examination of histone acetylation on Myc target genes using chromatin immunoprecipitation. J. Biol. Chem. 275:2000;33798-33805.
4. Grunstein M. Histone acetylation in chromatin structure and transcription. Nature. 389:1997;349-352.
5. Hebbes T.R., Thorne A.W., Crane-Robinson C. A direct link between core histone acetylation and transcriptionally active chromatin. EMBO J. 7:1988;1395-1402.
6. Hebbes T.R., Turner C.H., Thorne A.W., Crane-Robinson C. A "minimal epitope" anti-protein antibody that recognises a single modified amino acid. Mol. Immunol. 26:1989;865-873.
7. Hubbert C., Guardiola A., Shao R., Kawaguchi Y., Ito A., Nixon A., Yoshida M., Wang X.F., Yao T.P. HDAC6 is a microtubule-associated deacetylase. Nature. 417:2002;455-458.
8. Johnson G., Wu T.T. Kabat database and its applications: 30 years after the first variability plot. Nucleic Acids Res. 28:2000;214-218.
9. Johnson G., Wu T.T. Kabat database and its applications: future directions. Nucleic Acids Res. 29:2001;205-206.
10. Kohler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 256:1975;495-497.
11. Kouzarides T. Acetylation: a regulatory modification to rival phosphorylation? EMBO J. 19:2000;1176-1179.
12. MacRae T.H. Tubulin post-translational modifications - enzymes and their mechanisms of action. Eur. J. Biochem. 244:1997;265-278.
13. Matsuyama, A., Shimazu, T., Sumida, Y., Saito, A., Yoshimatsu, Y., Seigneurin-Berny, D., Osada, H., Komatsu, Y., Nishino, N., Khochbin, S., Horinouchi, S., Yoshida, M., In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. EMBO J. in press.
14. Muller S., Isabey A., Couppez M., Plaue S., Sommermeyer G., Van Regenmortel M.H. Specificity of antibodies raised against triacetylated histone H4. Mol. Immunol. 24:1987;779-789.
15. Narlikar G.J., Fan H.Y., Kingston R.E. Cooperation between complexes that regulate chromatin structure and transcription. Cell. 108:2002;475-487.
16. Pearson T., Galfre G., Ziegler A., Milstein C. A myeloma hybrid producing antibody specific for an allotypic determinant on "IgD-like" molecules of the mouse. Eur. J. Immunol. 7:1977;684-690.
17. Pfeffer U., Ferrari N., Vidali G. Availability of hyperacetylated H4 histone in intact nucleosomes to specific antibodies. J. Biol. Chem. 261:1986;2496-2498.
18. Rosenbaum J. Cytoskeleton: functions for tubulin modifications at last. Curr. Biol. 10:2000;R801-R803.
19. Ruff-Jamison S., Campos-Gonzalez R., Glenney J.R. Jr. Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features. J. Biol. Chem. 266:1991;6607-6613.
20. Sawada J., Mizusawa S., Terao T., Naito M., Kurosawa Y. Molecular characterization of monoclonal anti-steroid antibodies: primary structures of the variable regions of seven antibodies specific for 17 α-hydroxyprogesterone or 11-deoxycortisol and their pH-reactivity profiles. Mol. Immunol. 28:1991;1063-1072.
21. Sawada J., Yamazaki T., Terao T. Molecular and biochemical analyses of combining sites of monoclonal anti-morphine antibodies. Mol. Immunol. 30:1993;77-86.
22. Stern P.L., Willison K.R., Lennox E., Galfre G., Milstein C., Secher D., Ziegler A. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell. 14:1978;775-783.
23. Stoeva S., Schutz J., Gebauer W., Hundsdorfer T., Manz C., Markl J., Voelter W. Primary structure and unusual carbohydrate moiety of functional unit 2-c of keyhole limpet hemocyanin (KLH). Biochim. Biophys. Acta. 1435:1999;94-109.
24. Suka N., Suka Y., Carmen A.A., Wu J., Grunstein M. Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin. Mol. Cell. 8:2001;473-479.
25. Turner B.M., Fellows G. Specific antibodies reveal ordered and cell-cycle-related use of histone-H4 acetylation sites in mammalian cells. Eur. J. Biochem. 179:1989;131-139.
26. White D.A., Belyaev N.D., Turner B.M. Preparation of site-specific antibodies to acetylated histones. Methods. 19:1999;417-424.