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Volumn 13, Issue 5, 2003, Pages 947-950

Cancer chemotherapy: A SN-38 (7-Ethyl-10-hydroxycamptothecin) glucuronide prodrug for treatment by a PMT (Prodrug monoTherapy) strategy

Author keywords

[No Author keywords available]

Indexed keywords

7 ETHYL 10 HYDROXYCAMPTOTHECIN; BETA GLUCURONIDASE; PRODRUG;

EID: 0037430448     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(02)01080-6     Document Type: Article
Times cited : (22)

References (25)
  • 23
    • 85031229892 scopus 로고    scopus 로고
    • For the stability measurements, a solution of 250 μg/mL of prodrug in 0.02 M phosphate buffer (pH 7.2) was incubated at 37°C. Aliquots (40 μL) were taken at various times and analyzed by HPLC after dilution with eluent (120 μL). HPLC conditions: analysis was carried out on a reverse-phase column (Spherisorb C18) using isocratic conditions (1 mL/min) of 60% phosphate buffer (0.02 M, pH 3) and 40% acetonitrile with UV detection at 265 nm.
  • 25
    • 85031211938 scopus 로고    scopus 로고
    • For the kinetic measurements, a solution of 300 μM of prodrug in 0.02 M phosphate buffer (pH 7.2) was incubated at 37°C in the presence of 10 units/mL of β-D-glucuronidase (E. coli). Aliquots (40 μL) were taken at various times and analyzed by HPLC after dilution with eluent (120 μL). HPLC conditions: analysis was carried out on a reverse-phase column (WATERS X-terra, 250/4.6 mm) using isocratic conditions (1 mL/min) of 70% triethylammonium acetate buffer (0.1%, pH 9) and 30% acetonitrile with UV detection at 265 nm (extracted from PDA 3-D spectra).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.