-
2
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-
0028131354
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-
p-Nitrobenzyl esters were not viable substrates, with catalysis being either too ineffectual to detect reliably or absent altogether, suggesting that more activated acyl donors were required. Conveniently chromogenic p-nitrobenzyl esters were chosen because of their residual structural similarity to the hapten and, at the same time, higher reactivity than p-nitrobenzyl derivatives:
-
p-Nitrobenzyl esters were not viable substrates, with catalysis being either too ineffectual to detect reliably or absent altogether, suggesting that more activated acyl donors were required. Conveniently chromogenic p-nitrobenzyl esters were chosen because of their residual structural similarity to the hapten and, at the same time, higher reactivity than p-nitrobenzyl derivatives: Hirschmann R., Taylor C.M., Benkovic P.A., Taylor S.D., Yager K.M., Sprengler P.A., Smith A.B. III, Benkovic S.J. Science. 265:1994;234.
-
(1994)
Science
, vol.265
, pp. 234
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-
Hirschmann, R.1
Taylor, C.M.2
Benkovic, P.A.3
Taylor, S.D.4
Yager, K.M.5
Sprengler, P.A.6
Smith A.B. III7
Benkovic, S.J.8
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3
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85031208093
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-
note
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The peptide bond formation was completely inhibited by addition of an amount of hapten 1 equal to twice the antibody concentration.
-
-
-
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4
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0031052451
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Smithrud D.B., Benkovic P.A., Benkovic S.J., Taylor C.M., Yager K.M., Witherington J., Phillips B.W., Sprelenger P.A., Smith A.B. III, Hirschmann R. J. Am. Chem. Soc. 119:1997;278.
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(1997)
J. Am. Chem. Soc.
, vol.119
, pp. 278
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Smithrud, D.B.1
Benkovic, P.A.2
Benkovic, S.J.3
Taylor, C.M.4
Yager, K.M.5
Witherington, J.6
Phillips, B.W.7
Sprelenger, P.A.8
Smith A.B. III9
Hirschmann, R.10
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5
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0031053443
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Tawfik et al. subsequently reported p-nitrophenyl hydrolytic antibodies elicited by a p-nitrobenzyl phosphonate hapten:
-
Tawfik et al. subsequently reported p-nitrophenyl hydrolytic antibodies elicited by a p-nitrobenzyl phosphonate hapten: Tawfik D.S., Lindner A.B., Chap R., Eshhar Z., Green B.S. Eur. J. Biochem. 244:1997;619.
-
(1997)
Eur. J. Biochem.
, vol.244
, pp. 619
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Tawfik, D.S.1
Lindner, A.B.2
Chap, R.3
Eshhar, Z.4
Green, B.S.5
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6
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12944305948
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Smithrud D.B., Benkovic P.A., Benkovic S.J., Roberts V., Liu J., Neagu I., Iwama S., Phillips B., Smith A.B. III, Hirschmann R. Proc. Natl. Acad. Sci. U.S.A. 97:2000;1953.
-
(2000)
Proc. Natl. Acad. Sci. U.S.A.
, vol.97
, pp. 1953
-
-
Smithrud, D.B.1
Benkovic, P.A.2
Benkovic, S.J.3
Roberts, V.4
Liu, J.5
Neagu, I.6
Iwama, S.7
Phillips, B.8
Smith A.B. III9
Hirschmann, R.10
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7
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0028829779
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In contrast, enzyme-derived ligase, sublitigase, is incapable of cyclizing linear peptide precursors, containing less than 12 amino acids:
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In contrast, enzyme-derived ligase, sublitigase, is incapable of cyclizing linear peptide precursors, containing less than 12 amino acids: Jackson D.Y., Burnier J.P., Wells J.A. J. Am. Chem. Soc. 117:1995;819.
-
(1995)
J. Am. Chem. Soc.
, vol.117
, pp. 819
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Jackson, D.Y.1
Burnier, J.P.2
Wells, J.A.3
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8
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0028290693
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Schultz et al. subsequently disclosed generation of an abzyme-ligase:
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Schultz et al. subsequently disclosed generation of an abzyme-ligase: Jacobsen J.R., Schultz P.S. Proc. Natl. Acad. Sci. USA. 91:1994;5888.
-
(1994)
Proc. Natl. Acad. Sci. USA
, vol.91
, pp. 5888
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Jacobsen, J.R.1
Schultz, P.S.2
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12
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85031200522
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note
-
Both the desired and diasteriomeric dipeptide standards were prepared for the kinetic assay.
-
-
-
-
14
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85031202179
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-
note
-
The residual activity data was consistent with reversible inhibition.
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20
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0004214524
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London: Longmans, Green and Co
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Haldane J.B.S. Enzymes. 1930;Longmans, Green and Co, London.
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(1930)
Enzymes
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Haldane, J.B.S.1
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