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Volumn 13, Issue 7, 2003, Pages 1261-1264

The inhibitory effects of squalene-derived triterpenes on protein phosphatase PP2A

Author keywords

[No Author keywords available]

Indexed keywords

15,16 DEHYDROVENUSTATRIOL; 16 HYDROXYDEHYDROTHYRSIFEROL; CLAVIDOL; DEHYDROTHYRSIFEROL; DEHYDROVENUSTATRIOL; DIOXEPANDEHYDROTHYRSIFEROL; ISODEHYDROTHYRSIFEROL; LACTODEHYDROTHYRSIFEROL; PHOSPHOPROTEIN PHOSPHATASE; SQUALENE DERIVATIVE; THYRSENOL; THYRSENOL A; THYRSENOL B; THYRSIFERYL 23 ACETATE; TRITERPENE DERIVATIVE; UNCLASSIFIED DRUG;

EID: 0037424699     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(03)00136-7     Document Type: Article
Times cited : (22)

References (17)
  • 1
    • 0028838971 scopus 로고
    • Hunter T. Cell. 80:1995;225.
    • (1995) Cell , vol.80 , pp. 225
    • Hunter, T.1
  • 15
    • 85031205004 scopus 로고    scopus 로고
    • note
    • 2, pH 7.0) and 20 μL of PP2A diluted in Tris-HCl pH 7 (0.025 u/well). The blank was prepared in the same way as the sample but with no enzyme (30 μL of Tris-HCl pH 7); there were at least eight blanks per plate. The reaction mixture was incubated for 15 min at 37°C, sample (10 μL) was added except to the control and blank wells. This mixture was again incubated for 15 min at 37°C and then the fluorescent substrate (FDP) was added in 120 μL as an assay buffer at a final concentration of 15 μM. Each determination was carried out in triplicate and each sample was tested in four or five different dilutions. The reaction was incubated for 30 min at 37°C and fluorescence was measured with a microplate reader fluorometer set at 492 nm (exc) and 514 nm (em).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.