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Volumn 32, Issue 1, 2003, Pages 88-89

Photo-enhancement of transfection efficiency with a novel azobenzene-based cationic lipid

Author keywords

[No Author keywords available]

Indexed keywords

AZOBENZENE; DNA; LIPID;

EID: 0037419572     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2003.88     Document Type: Article
Times cited : (20)

References (27)
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    • note
    • 2O: C, 56.11; H, 9.08; N, 11.90%. Found: C, 56.41, H, 9.01; N, 11.93%.
  • 24
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    • note
    • KAON12 was dissolved in a chloroform/MeOH (50/50) solution, and by the use of an evaporator, a thin membrane was formed along the wall of a glass tube. Then, by the use of a voltex, the thin membrane was subjected to ultrasound at 50°C and dispersed in a Tris buffer (pH: 7.5) to attempt lipid aggregate formation.
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    • note
    • Various amounts of cationic compounds were mixed to fixed amount (150 ng) of pGL3-control in order to obtain charge ratio (+/-) ranging from 0 to 3.2. After 30 min incubation at 37°C, the complexes were electrophoresed through a 0.8% agarose gel for 45 min at 100V. DNAs were stained by ethidium bromide.
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    • note
    • 2) was irradiated for zero or ten minutes, and the results were compared. The measurement of transfection efficiencies was performed in triplicate.


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