Heterologous production of Halorhodospira halophila holo-photoactive yellow protein through tandem expression of the postulated biosynthetic genes

Biochemistry

Volumn 42, Issue 4, 2003, Pages 965-970

  Kynd, John A ^a   Vanrobaeys, Frank ^a   Fitch, John C ^b   Devreese, Bart V ^a   Meyer, Terrance E ^b   Cusanovich, Michael A ^b   Van Beeumen, Jozef J ^a  

^a GHENT UNIVERSITY   (Belgium)

^b University of Arizona   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; ESCHERICHIA COLI; GENES; ORGANIC ACIDS;

PHOTOACTIVE YELLOW PROTEINS (PYP); PHOTORECEPTORS;

PROTEINS;

AMMONIA LYASE; CYSTEINE; LIGASE; PARA COUMARIC ACID; PHOTOACTIVE YELLOW PROTEIN; TYROSINE;

ARTICLE; COVALENT BOND; ESCHERICHIA COLI; GENE EXPRESSION; HALORHODOSPIRA HALOPHILA; MUTAGENESIS; NONHUMAN; OPERON; PHOTORECEPTOR; PHOTOTROPHIC BACTERIUM; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN SYNTHESIS; SIGNAL TRANSDUCTION;

AMMONIA-LYASES; APOPROTEINS; BACTERIAL PROTEINS; CHROMATIACEAE; CLONING, MOLECULAR; COUMARIC ACIDS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, BACTERIAL; HYDRO-LYASES; KINETICS; LASERS; LIGHT; PHOTOCHEMISTRY; PHOTORECEPTORS, MICROBIAL; PLASMIDS; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HALOPHILA; HALORHODOSPIRA HALOPHILA; PHOTOBACTERIA;

EID: 0037417745     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi027037b     Document Type: Article

References (23)

1. Meyer, T. E. (1985) Isolation and characterization of soluble cytochromes, ferredoxins and other chromophoric proteins from the halophilic phototrophic bacterium Ectothiorhodospira halophila, Biochim. Biophys. Acta 806, 175-183.
2. Sprenger, W. W., Hoff, W. D., Armitage, J. P., and Hellingwerf, K. J. (1993) The eubacterium Ectothiorhodospira halophila is negatively phototactic, with a wavelength dependence that fits the absorption spectrum of the photoactive yellow protein, J. Bacteriol. 175, 3096-3104.
3. Koh, M., van Driessche, G., Samyn, B., Hoff, W. D., Meyer, T. E., Cusanovich, M. A., and Van Beeumen, J. J. (1996) Sequence evidence for strong conservation of the photoactive yellow proteins from the halophilic phototrophic bacteria Chromatium salexigens and Rhodospirillum salexigens, Biochemistry 35, 2526-2534.
4. Kort, R., Hoff, W. D., van West, M., Kroon, A. R., Hoffer, S. M., Vlieg, K. H., Crielaard, W., Van Beeumen, J. J., and Hellingwerf, K. J. (1996) The xanthopsins: a new family of eubacterial blue-light photoreceptors, EMBO J. 15, 3209-3218.
5. Haselkorn, R., Lapidus, A., Kogan, Y., Vlcek, C., Paces, J., Paces, V., Ulbrich, P., Pecenkova, T., Rebrekov, D., Milgram, A., Mazur, M., Cox, R., Kyrpides, N., Ivanova, N., Kapatral, V., Los, T., Lykidis, A., Mikhailova, N., Reznik, G., Vasieva, O., and Fonstein, M. (2002) The Rhodobacter capsulatus genome, Photosynth. Res. 70, 43-52.
6. Haker, A., Hendriks, J., Gensch, T., Hellingwerf, K., and Crielaard, W. (2000) Isolation, reconstitution and functional characterization of the Rhodobacter sphaeroides photoactive yellow protein, FEBS Lett. 486, 52-56.
7. Jiang, Z., Swem, L. R., Rushing, B. G., Devanathan, S., Tollin, G., and Bauer, C. E. (1999) Bacterial photoreceptor with similarity to photoactive yellow protein and plant phytochromes, Science 285, 406-409.
8. Pellequer, J., Wager-Smith, K. A., Kay, S. A., and Getzoff, E. D. (1998) Photoactive yellow protein: A structural prototype for the three-dimensional fold of the PAS domain superfamily, Proc. Natl. Acad. Sci. U.S.A. 95, 5884-5890.
9. Borgstahl, G. E. O., Williams, D. R., and Getzoff, E. D. (1995) 1.4 Å structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore, Biochemistry 34, 6278-6287.
10. Ujj, L., Devanathan, S., Meyer, T. E., Cusanovich, M. A., Tollin, G., and Atkinson, G. H. (1998) New photocycle intermediates in the photoactive yellow protein from Ectothiorhodospira halophila: picosecond transient absorption spectroscopy, Biophys. J. 75, 406-412.
11. Perman, B., Srajer, V., Ren, Z., Teng, T., Pradervand, C., Ursly, T., Bourgois, D., Schotte, F., Wulff, M., Kort, R., Hellingwerf, K., and Moffat, K. (1998) Energy transduction on the nanosecond time scale: early structural events in a xanthopsin photocycle, Science 279, 1946-1950.
12. Genick, U. K., Soltis, S. M., Kuhn, P., Canestrelli, I. L., and Getzoff, E. D. (1998) Structure at 0.85 Å resolution of an early protein photocycle intermediate, Nature 392, 206-209.
13. Baca, M., Borgstahl, G. E. O., Boissinot, M., Burke, P. M., Williams, D. R., Slater, K. A., and Getzoff, E. D. (1994) Complete chemical structure of photoactive yellow protein: novel thioesterlinked 4-hydroxycinnamyl chromophore and photocycle chemistry, Biochemistry 33, 14369-14377.
14. Hoff, W. D., Düx, P., Hard, K., Devreese, B., Nugteren-Roodzant, I. M., Crielaard, W., Boelens, R., Kaptein, R., Van Beeumen, J. J., and Hellingwerf, K. J. (1994) Thiol ester-linked p-coumaric acid as a new photoactive prosthetic group in a protein with rhodopsin-like photochemistry, Biochemistry 33, 13959-13962.
15. Devanathan, S., Pacheco, A., Ujj, L., Cusanovich, M., Tollin, G., Lin, S., and Woodbury, N. (1999) Femtosecond spectroscopic observations of initial intermediates in the photocycle of the photoactive yellow protein from Ectothiorhodospira halophila, Biophys. J. 77, 1017-1023.
16. Imamoto, Y., lto, T., Kataoka, M., and Tokunga, F. (1995) Reconstitution photoactive yellow protein from apoprotein and p-coumaric acid derivatives, FEBS Lett. 374, 157-160.
17. Hahlbrock, K., and Scheel, D. (1989) Physiology and molecular biology of phenylpropanoid metabolism, Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 347-369.
18. Kyndt, J. A., Meyer, T. E., Cusanovich, M. A., and Van Beeumen, J. J. (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein, FEBS Lett. 512, 240-244.
19. Kort, R., Phillips-Jones, M. K., van Aalten, D. M., Haker, A., Hoffer, S. M., Hellingwerf, K. J., and Crielaard, W. (1998) Sequence, chromophore extraction and 3-D model of the photoactive yellow protein from Rhodobacter sphaeroides, Biochim. Biophys. Acta 1385, 1-6.
20. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. Genick, U. K., Devanathan, S., Meyer, T. E., Canestrelli, I. L., Williams, E., Cusanovich, M. A., Tollin, G., and Getzoff, E. D. (1997) Active site mutants implicate key residues for control of color and light cycle kinetics of photoactive yellow protein, Biochemistry 36, 8-14.
22. Meyer, T. E., Yakali, E., Cusanovich, M. A., and Tollin, G. (1987) Properties of a water-soluble, yellow protein isolated from a halophilic phototrophic bacterium that has photochemical activity analogous to sensory rhodopsin, Biochemistry 26, 418-423.
23. Van Beeumen, J. J., Devreese, B., Van Bun, S., Hoff, W. D., Hellingwerf, K. J., Meyer, T. E., McRee, D. E., and Cusanovich, M. A. (1993) Primary structure of a photoactive yellow protein from the phototrophic bacterium Ectothiorhodospira halophila, with evidence for the mass and the binding site of the chromophore, Protein Sci. 2, 1114-1125.