Assessment of cell concentration and viability of isolated hepatocytes using flow cytometry

Analytical Biochemistry

Volumn 317, Issue 1, 2003, Pages 19-25

  Wigg, Alan J ^a   Phillips, John W ^a   Wheatland, Loretta ^b   Berry, Michael N ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b FLINDERS MEDICAL CENTRE   (Australia)

Author keywords

ATP; Flow cytometry; Hemacytometry; Isolated hepatocytes; Metabolism; Propidium iodide; TRUCOUNT Tubes; Trypan blue

Indexed keywords

ADENOSINETRIPHOSPHATE; AMMONIA; CELLS; COUNTING TUBES; CYTOLOGY; FLOW CYTOMETRY; METABOLISM; UREA;

CELL CONCENTRATIONS; CYTOCHROME P450; HEMACYTOMETRY; HEPATOCYTES; LACTATE DEHYDROGENASE; NEUBAUER COUNTING CHAMBER; PROPIDIUM IODIDE; TRYPAN BLUE;

SUSPENSIONS (FLUIDS);

ADENOSINE TRIPHOSPHATE; ALBUMIN; AMMONIA; CYTOCHROME P450; LACTATE DEHYDROGENASE; LACTIC ACID; PROPIDIUM IODIDE; TRYPAN BLUE; UREA;

ACCURACY; ANIMAL CELL; ARTICLE; CELL COUNT; CELL FUNCTION; CELL ISOLATION; CELL LEVEL; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; LIVER CELL; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT; REPRODUCIBILITY;

EID: 0037410069     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00057-5     Document Type: Article

References (17)

1. J.V. Dacie, S.M. Lewis, Practical Haematology, J & A Churchill Ltd., London, 1963, pp. 18-45.
2. Pogson C.I., Elliot K.R.F., Lomax M.A., Munoz-Clare R.A., Smith S.A. Harris R.A., Cornell N.W. Isolation, Characterization and Use of Hepatocytes. 1983;21-30 Elsevier, New York.
3. Page R.A., Stowell K.M., Hardman M.J., Kitson K.E. Anal. Biochem. 200:1992;171-175.
4. Coder D. Robinson P.J., Dorzynkiewicz Z., Dean P.N., Hills A.R., Orfao A., Rabinovitch P.S., Wheeless L.L. Current Protocols in Cytometry. vol. 2:1997;9.2.1-9.2.14 Wiley, Inc. New York.
5. Nicholson J.K., Stein D., Mui T., Mack R., Hubbard M., Denny T. Clin. Diagn. Lab. Immunol. 4:1997;309-313.
6. Chen R.F. J. Biol. Chem. 242:1967;173-181.
7. Berry M.N., Edwards A.M., Barritt G.J. Burdon R.H., Knippenberg P.H. Isolated Hepatocytes: Preparation, Properties and Applications. 1991;15-58 Elsevier, Amsterdam.
8. Berry M.N., Friend D.S. J. Cell Biol. 43:1969;506-520.
9. Berry M.N., Phillips J.W., Grivell M.B., Efthivoulou M.A., Ribstein M.C. Crepaldi G., Demetriou A.A., Muraca M. Bioartificial Liver Support: The Critical Issues. 1997;70-81 CIC edizioni internationale, Rome.
10. Bergmeyer H.U. Methods of Enzymatic Analysis. 1974;Academic Press, New York.
11. Schenkman J.B., Jansson I. Phillips I.R., Shephard E.A. Methods in Molecular Biology: Cytochrome P450 Protocols. vol. 107:1998;25-33 Humana Press Inc. Totowa, NJ.
12. Berry M.N., Gregory R.B., Grivell A.R., Henly D.C., Nobes C.D., Phillips J.W., Wallace P.G. Biochim. Biophys. Acta. 936:1988;294-306.
13. Yagi T., Hardin J.A., Valenzuela Y.M., Miyoshi H., Gores G.J., Nyberg S.L. Hepatology. 33:2001;1432-1440.
14. Berry M.N., Edwards A.M., Barritt G.J. Burdon R.H., van Knippenberg P.H. Isolated Hepatocytes Preparation, Properties and Applications. vol. 21:1991;121-178 Elsevier, Amsterdam.
15. Otto D.A., Cook G.A., Reiss P.D. Harris R.A., Cornell N.W. Isolation, Characterization and Use of Hepatocytes. 1983;41 Elsevier Biomedical, New York.
16. Park J.G., Kramer B.S., Steinberg S.M., Carmichael J., Collins J.M., Minna J.D., Gazdar A.F. Cancer Res. 47:1987;5875-5879.
17. T. Riss, R. Moravec, M. Beck, R. Hannah, K. Wilson, R. Swanson, in: Promega Notes Magazine, vol. 81, 2002, pp. 2-5.