Novel use of the isoepoxydon dehydrogenase gene probe of the patulin metabolic pathway and chromatography to test penicillia isolated from apple production systems for the potential to contaminate apple juice with patulin

Food Microbiology

Volumn 20, Issue 3, 2003, Pages 359-364

  Paterson, R R M ^a   Kozakiewicz, Z ^a   Locke, T ^a,b   Brayford, D ^a   Jones, S C B ^a,c  

^a CABI   (United Kingdom)

^b ADAS BOXWORTH   (United Kingdom)

^c LONZA AG   (Switzerland)

Author keywords

Gene probe; Metabolic pathway; Penicillia

Indexed keywords

FUNGI; MALUS X DOMESTICA; PENICILLIUM BREVICOMPACTUM; PENICILLIUM EXPANSUM; PENICILLIUM ROQUEFORTI; PENICILLIUM ROQUEFORTII;

EID: 0037409445     PISSN: 07400020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0740-0020(02)00087-4     Document Type: Article

References (7)

1. FDA, 2001. Patulin in apple juice, apple juice concentrates and apple juice products. US Food and Drug Administration, September 15, 2001, 11pp. http://www.cfsan.fda.gov/~dms/patubck2.html
2. Fedeshko, R.W., 1992. Polyketide enzymes and genes. Ph.D. Thesis, Vols. 1 & 2, University of Calgary, Canada. Call No.= QP752.P65F431992.
3. Paterson, R.R.M., Bridge, P.D., 1994. Biochemical techniques for filamentous fungi. IMI Techniques Series No. 1, CAB International, Wallingford, 125pp.
4. Paterson, R.R.M., Archer, S., Kozakiewicz, Z., Lea, A., Locke, T., O'Grady, E., 2000. A gene probe for the patulin metabolic pathway with potential use in novel disease control. Biocontrol Sci. Technol. 10, 509-512.
5. Steiman, R., Seigle-Murandi, F., Sage, L., Krivobok, S., 1989. Production of patulin by micromycetes. Mycopathology 105, 129-133.
6. Sydenham, E.W., Vismer, H.F., Marasas, W.F.O., Brown, N.L., Schlechter, M., Rheeder, J.P., 1997. The influence of deck storage and initial processing on patulin levels in apple juice. Food Addit. Contam. 14, 429-434.
7. Wang, I.-K., Reeves, C., Gaucher, G.M., 1991. Isolation and sequencing of a genomic DNA clone containing the 3′ terminus of the 6-methylsalicylic acid polyketide synthesis gene of Penicillium urticae. Can. Microbiol. 37, 86-95.