5α-Reductase isoenzymes 1 and 2 in the rat testis during postnatal development

Biology of Reproduction

Volumn 68, Issue 5, 2003, Pages 1711-1718

  Killian, Jessica ^a   Pratis, Kyriakos ^a   Clifton, Rebecca J ^a   Stanton, Peter G ^a   Robertson, David M ^a   O'Donnell, Liza ^a  

^a HUDSON INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

Pituitary hormones; Spermatogenesis; Steroid hormones; Testis; Testosterone

Indexed keywords

21,21 PENTAMETHYLENE 4 AZA 5ALPHA PREGN 1 ENE 3,20 DIONE; ANDROGEN; HYPOPHYSIS HORMONE; ISOENZYME; L 685273; MESSENGER RNA; STEROID 5ALPHA REDUCTASE; STEROID 5ALPHA REDUCTASE 1; STEROID 5ALPHA REDUCTASE 2; STEROID HORMONE; TESTOSTERONE; UNCLASSIFIED DRUG; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; ENZYME INHIBITOR; FOLLITROPIN; GONADOTROPIN; LUTEINIZING HORMONE; PREGNANE DERIVATIVE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOSYNTHESIS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HORMONE SYNTHESIS; MALE; METABOLITE; NONHUMAN; POSTNATAL DEVELOPMENT; PRIORITY JOURNAL; PUBERTY; RADIOIMMUNOASSAY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATOGENESIS; TESTIS; ANIMAL; BLOOD; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; GROWTH, DEVELOPMENT AND AGING; METABOLISM; NEWBORN; ORGAN SIZE; PROSTATE; SPRAGUE DAWLEY RAT;

ANIMALIA;

ANDROGENS; ANIMALS; ANIMALS, NEWBORN; ENZYME INHIBITORS; FOLLICLE STIMULATING HORMONE; GONADOTROPINS; INDICATORS AND REAGENTS; ISOENZYMES; LUTEINIZING HORMONE; MALE; ORGAN SIZE; PREGNENEDIONES; PROSTATE; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPERMATOGENESIS; TESTIS; TESTOSTERONE 5-ALPHA-REDUCTASE;

EID: 0037407746     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod.102.009142     Document Type: Article

References (37)

1. Singh J, O'Neill C, Handelsman DJ. Induction of spermatogenesis by androgens in gonadotropin-deficient (hpg) mice. Endocrinology 1995; 136:5311-5321.
2. Matsumoto K, Yamada M. 5α-Reduction of testosterone in vitro by rat seminiferous tubules and whole testes at different stages of development. Endocrinology 1973; 93:253-255.
3. Corpechot C, Baulieu EE, Robel P. Testosterone, dihydrotestosterone and androstanediols in plasma, testes and prostates of rats during development. Acta Endocrinol (Copenh) 1981; 96:127-135.
4. Van der Molen H, Grootegoed J, de Greef-Bijeveld M, Rommerts F, van der Vuuse G. Distribution of steroids, steroid production and steroid metabolizing enzymes in rat testis. In: French F, Hansson V, Ritzen E, Nayfey S (eds.), Hormonal Regulation of Spermatogenesis. New York: Plenum Press; 1975: 3-23.
5. Mahendroo MS, Cala KM, Hess DL, Russell DW. Unexpected virilization in male mice lacking steroid 5α-reductase enzymes. Endocrinology 2001; 142:4652-4662.
6. Grino PB, Griffin JE, Wilson JD. Testosterone at high concentrations interacts with the human androgen receptor similarly to dihydrotestosterone. Endocrinology 1990; 126:1165-1172.
7. Deslypere JP, Young M, Wilson JD, McPhaul MJ. Testosterone and 5α-dihydrotestosterone interact differently with the androgen receptor to enhance transcription of the MMTV-CAT reporter gene. Mol Cell Endocrinol 1992; 88:15-22.
8. Russell DW, Wilson JD. Steroid 5α-reductase: two genes/two enzymes. Annu Rev Biochem 1994; 63:25-61.
9. Thigpen AE, Cala KM, Russell DW. Characterization of Chinese hamster ovary cell lines expressing human steroid 5α-reductase isozymes. J Biol Chem 1993; 268:17404-17412.
10. Normington K, Russell DW. Tissue distribution and kinetic characteristics of rat steroid 5α-reductase isozymes. Evidence for distinct physiological functions. J Biol Chem 1992; 267:19548-19554.
11. Lopez-Solache I, Luu-The V, Seralini GE, Labrie F. Heterogeneity of rat type I 5α-reductase cDNA: cloning, expression and regulation by pituitary implants and dihydrotestosterone. Biochim Biophys Acta 1996; 1305:139-144.
12. Horton R, Pasupuletti V, Antonipillai I. Androgen induction of steroid 5α-reductase may be mediated via insulin-like growth factor-I. Endocrinology 1993; 133:447-451.
13. Antonipillai I, Wahe M, Yamamoto J, Horton R. Activin and inhibin have opposite effects on steroid 5α-reductase activity in genital skin fibroblasts. Mol Cell Endocrinol 1995; 107:99-104.
14. 3H-testosterone by the seminiferous tubules and interstitial tissue from immature and mature rat testes. Endocrinology 1973; 92:41-47.
15. Dorrington JH, Fritz IB. Cellular localization of 5α-reductase and 3αhydroxysteroid dehydrogenase in the seminiferous tubule of the rat testis. Endocrinology 1975; 96:879-889.
16. Viger RS, Robaire B. Steady-state steroid 5α-reductase messenger ribonucleic acid levels and immunocytochemical localization of the type 1 protein in the rat testis during postnatal development. Endocrinology 1995; 136:5409-5415.
17. Thigpen AE, Silver RI, Guileyardo JM, Casey ML, McConnell JD, Russell DW. Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. J Clin Invest 1993; 92:903-910.
18. Reyes EM, Camacho-Arroyo I, Nava G, Cerbon MA. Differential methylation in steroid 5α-reductase isozyme genes in epididymis, testis, and liver of the adult rat. J Androl 1997; 18:372-377.
19. O'Donnell L, Pratis K, Stanton PG, Robertson DM, McLachlan RI. Testosterone-dependent restoration of spermatogenesis in adult rats is impaired by a 5α-reductase inhibitor. J Androl 1999; 20:109-117.
20. O'Donnell L, Stanton PG, Wreford NG, Robertson DM, McLachlan RI. Inhibition of 5α-reductase activity impairs the testosterone-dependent restoration of spermiogenesis in adult rats. Endocrinology 1996; 137:2703-2710.
21. Pratis K, O'Donnell L, Ooi GT, McLachlan RI, Robertson DM. Enzyme assay for 5α-reductase type 2 activity in the presence of 5αreductase type 1 activity in rat testis. J Steroid Biochem Mol Biol 2000; 75:75-82.
22. Saito K, O'Donnell L, McLachlan RI, Robertson DM. Spermiation failure is a major contributor to early spermatogenic suppression caused by hormone withdrawal in adult rats. Endocrinology 2000; 141:2779-2785.
23. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000; 25:169-193.
24. Haavisto AM, Pettersson K, Bergendahl M, Perheentupa A, Roser JF, Huhtaniemi I. A supersensitive immunofluorometric assay for rat luteinizing hormone. Endocrinology 1993; 132:1687-1691.
25. Zhengwei Y, Wreford NG, de Kretser DM. A quantitative study of spermatogenesis in the developing rat testis. Biol Reprod 1990; 43:629-635.
26. Russell LD, Alger LE, Nequin LG. Hormonal control of pubertal spermatogenesis. Endocrinology 1987; 120:1615-1632.
27. O'Shaughnessy PJ, Murphy L. Steroidogenic enzyme activity in the rat testis following Leydig cell destruction by ethylene-1,2-dimethanesulphonate and during subsequent Leydig cell regeneration. J Endocrinol 1991; 131:451-457.
28. Yoshizaki K, Matsumoto K, Samuels LT. Localization of δ4-5α-reductase in immature rat testes. Endocrinology 1978; 102:918-925.
29. Murono EP, Washburn AL. Regulation of 5α-reductase activity in cultured immature Leydig cells by human chorionic gonadotropin. J Steroid Biochem 1990; 35:715-721.
30. 14C. Endocrinology 1972; 90:618-623.
31. Aumuller G, Eicheler W, Renneberg H, Adermann K, Vilja P, Forssmann WG. Immunocytochemical evidence for differential subcellular localization of 5α-reductase isoenzymes in human tissues. Acta Anat (Basel) 1996; 156:241-252.
32. Dorrington JH, Fritz IB. Metabolism of testosterone by preparations from the rat testis. Biochem Biophys Res Commun 1973; 54:1425-1431.
33. Pratis K, O'Donnell L, Ooi GT, McLachlan RI, Robertson DM. Differential regulation of 5α-reductase type 1 and type 2 activity in rat testis. In: 11th International Congress of Endocrinology; 2000; Sydney, Australia. Abstract P197.
34. Nayfeh SN, Coffey JC, Hansson V, French FS. Maturational changes in testicular steroidogenesis: hormonal regulation of 5α-reductase. J Steroid Biochem 1975; 6:329-335.
35. George FW, Johnson L, Wilson JD. The effect of a 5α-reductase inhibitor on androgen physiology in the immature male rat. Endocrinology 1989; 125:2434-2438.
36. Canovatchel WJ, Volquez D, Huang S, Wood E, Lesser ML, Gautier T, Imperato-McGinley J. Luteinizing hormone pulsatility in subjects with 5α-reductase deficiency and decreased dihydrotestosterone production. J Clin Endocrinol Metab 1994; 78:916-921.
37. Ge RS, Hardy MP. Variation in the end products of androgen biosynthesis and metabolism during postnatal differentiation of rat Leydig cells. Endocrinology 1998; 139:3787-3795.