Expression and localization of organic cation/carnitine transporter OCTN2 in Caco-2 cells

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 284, Issue 5 47-5, 2003, Pages

  Elimrani, Ihsan ^a   Lahjouji, Karim ^a   Seidman, Ernest ^a   Roy, Marie Josée ^a   Mitchell, Grant A ^a   Qureshi, Ijaz ^a  

^a UNIVERSITY OF MONTREAL   (Canada)

Author keywords

Anti OCTN2 antibodies; Brush border membrane; Intestine; Organic cation transport; Valproate

Indexed keywords

CARNITINE; CARRIER PROTEIN; CATION; MEPYRAMINE; ORGANIC CATION CARNITINE TRANSPORTER; PROLINE; TETRYLAMMONIUM; UNCLASSIFIED DRUG; VALPROIC ACID; VERAPAMIL;

ACTIVE TRANSPORT; ARTICLE; BIOSYNTHESIS; BRUSH BORDER; COLON ADENOCARCINOMA; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INTESTINE ABSORPTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTAKE; PROTEIN LOCALIZATION; PROTEIN TRANSPORT;

EID: 0037403719     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.00220.2002     Document Type: Article

References (58)

1. +-dependent sugar transport in Caco-2 cells and human fetal colon. J Membr Biol 99: 113-125, 1987.
2. +-dependent organic cation transport mechanism. Br J Pharmacol 129: 619-625, 2000.
3. Bremer J. Carnitine metabolism and functions. Physiol Rev 3: 1420-1480, 1983.
4. Burwinkel B, Kreuder J, Schweitzer S, Vorgerd M, Gempel K, Gerbitz KD, and Kilimann MW. Carnitine transporter OCTN2 mutations in systemic primary carnitine deficiency: a novel Arg169Gln mutation and a recurrent Arg282ter mutation associated with an unconventional splicing abnormality. Biochem Biophys Res Commun 261: 484-487, 1999.
5. Campistol J, Chavez B, Vilaseca MA, and Artuch R. Anti-epileptic drugs and carnitine. Rev Neurol, Suppl 1: S105-S109, 2000.
6. Duran JM, Peral MJ, Calonge MI, and Ilundain AA. Functional characterization of intestinal L-carnitine transport. J Membr Biol 185: 65-74, 2002.
7. Gross CJ, Henderson LM, and Savaiano DA. Uptake of L-carnitine and acetyl-L-carnitine by isolated guinea-pig enterocytes. Biochim Biophys Acta 886: 425-433, 1986.
8. Gudjonson H, Li BUK, Shug AL, and Olsen WA. In vivo studies of intestinal carnitine absorption in rats. Gastroenterology 88: 1880-1887, 1985.
9. Hamilton JW, Li BU, Shug AL, and Olson WA. Carnitine transport in human intestinal biopsy specimens. Gastroenterology 91: 10-16, 1986.
10. Hauri HP, Sterchi EE, Bienz D, Fransen JAM, and Marxer A. Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. J Cell Biol 101:838-851, 1985.
11. Hidalgo IJ and Borchart RT. Transport of a large neutral amino acid (phenylalanine) in a human intestinal epithelial cell line: Caco-2. Biochim Biophys Acta 1028: 25-30, 1990.
12. Hidalgo IJ and Borchart RT. Transport of bile acids in human intestinal epithelial cell line, Caco-2. Biochim Biophys Acta 1035:97-103, 1990.
13. Hidalgo IJ, Raub TJ, and Borchardt RT. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96: 736-749, 1989.
14. Hirohashi T, Suzuki H, Chu XY, Tamai I, Tsuji A, and Sugiyama Y. Function and expression of multidrug resistance-associated protein family in human colon adenomacarcinoma cells (Caco-2). J Pharmacol Exp Ther 292: 265-270, 2000.
15. Kempson SA, Kim JK, Northrup TE, Knox FG, and Dousa TP. Alkaline phosphatase in adaptation to low dietary phosphate intake. Am J Physiol 237: E465-E473, 1980.
16. Koepsell H. Organic cation transporters in intestine, kidney, liver and brain. Annu Rev Physiol 60: 243-266, 1998.
17. Koizumi A, Nozaki JI, Ohura T, Kayo T, Wada Y, Nezu JI, Ohashi R, Tamai I, Shoji Y, Takada G, Kibira S, Matsuishi T, and Tsuji A. Genetic epidemiology of the carnitine transporter OCTN2 gene in Japanese pedigrees with primary systemic carnitine deficiency. Hum Mol Genet 8: 2247-2254, 1999.
18. Lahjouji K, Malo C, Mitchell GA, and Qureshi IA. L-Carnitine transport in mouse renal and intestinal brush-border and basolateral membrane vesicles. Biochim Biophys Acta 1558: 82-93, 2002.
19. Lahjouji K, Mitchell GA, and Qureshi IA. Carnitine transport by organic cation transporters and systemic carnitine deficiency. Mol Genet Metab 73: 287-297, 2001.
20. Levy E, Mehran M, and Seidman E. Caco-2 cells as a model for intestinal lipoprotein synthesis and secretion. FASEB J 9: 626-635, 1995.
21. Li BU, Bummer PM, Hamilton JW, Gudjonsson H, Zografi G, and Olson WA. Uptake of L-carnitine by rat jejunal brush border microvillus membrane vesicles. Dig Dis Sci 35: 333-339, 1990.
22. Lloyd JB and Whelan WJ. An improved method for enzyme determination of glucose in the presence of maltose. Anal Biochem 30: 467-470, 1969.
23. Lowry FH, Rosenbrough NJ, Farr AL, and Randall RF. Protein measurement with Folin phenol reagent. J Biol Chem 193:269-275, 1951.
24. Mahraoui L, Rousset M, Dussaulx E, Darmout D, Zweibaum A, and Brot-Laroche E. Expression and localization of GLUT-5 in Caco-2 cells, human small intestine. Am J Physiol Gastrointest Liver Physiol 263: G312-G318, 1992.
25. McCloud E, Ma TY, Grant KE, Mathis RK, and Said HM. Uptake of L-carnitine by a human intestinal epithelial cell line, Caco-2. Gastroenterology 111: 1534-1540, 1996.
26. Messer M and Dahqvist A. A one step ultramicro method for the essay of intestinal disaccharidases. Anal Biochem 14: 376-392, 1966.
27. +-ATPase rich membranes and the distribution of enzyme activities. J Membr Biol 28: 309-333, 1976.
28. 0,+ from mouse colon expressed in HRPE cells and Xenopus oocytes. J Physiol 532: 297-304, 2001.
29. Nakanishi T, Kekuda R, Hatanaka T, Huang W, Sugawara M, Martindale RG, Leibach FH, Prasad PD, and Ganapathy V. Cloning and functional characterization of new subtype of the amino acid transport system. Am J Physiol Cell Physiol 281: C1757-C1768, 2001.
30. Nezu J, Tamai I, Oku A, Ohashi R, Yabuuchi H, Hashimoto N, Nikaido H, Sai Y, Koizumi A, Shoji Y, Takada G, Matsuishi T, Yoshino M, Kato H, Ohura T, Tsujimoto G, Hayakawa JI, Shimane M, and Tsuji A. Primary systemic carnitine deficiency is caused by mutations in a gene encoding sodium ion-dependent carnitine transporter. Nat Genet 21: 91-94, 1999.
31. Nicklin PL, Irwi WJ, Hassa IF, and Mackay M. Proline uptake by monolyers of human intestinal absorptive (Caco-2) cells. Biochim Biophys Acta 1104: 283-292, 1992.
32. Ohashi R, Tamai I, Nezu JI, Nikaido H, Hashimoto N, Oku A, Sai Y, Shimane M, and Tsuji A. Molecular and physiological evidence for multifunctionality of carnitine/organic cation transporter OCTN2. Mol Pharmacol 59: 358-366, 2001.
33. +-dependent carnitine transport by organic cation transporter (OCTN2): its pharmacological and toxicological relevance. J Pharmacol Exp Ther 291: 778-784, 1999.
34. Pinto M, Robine-Léon S, Appay MD, Kedinger M, Triadou N, Dussaulx E, Lacroix B, Simon-Assmann P, Halffen K, Fogh J, and Sweilbaum A. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell 47: 323-330, 1983.
35. Pons R and DeVivo DC. Primary and secondary carnitine deficiency syndromes. J Child Neurol 10: 8-24, 1995.
36. Raskind JY and El-Chaar GM. The role of carnitine supplementation during valproic acid therapy. Ann Phamacother 34: 630-638, 2000.
37. Schmitz J, Preiser H, Maestracci D, Ghosh BK, Cerda JJ, and Crane RK. Purification of the human intestinal brush border membrane. Biochim Biophys Acta 323: 98-112, 1973.
38. Sekine T, Kusuhara H, Utsunomiya-Tte N, Tsuda M, Sugiyama Y, Kanai Y, and Endou H. Molecular cloning and characterization of high-affinity carnitine transporter from rat intestine. Biochim Biophys Acta 251: 586-591, 1998.
39. Shaw RD, Li BUK, Hamilton JW, Shug AL, and Olsen WA. Carnitine transport in rat small intestine. Am J Physiol Gastrointest Liver Physiol 245: G376-G381, 1983.
40. Takeuchi T, Sugimoto T, Nishida N, and Kobayashi Y. Evaluation of cytotoxicity of sodium valproate on primary cultured rat hepatocytes. Neuropediatrics 19: 158-161, 1988.
41. Tamai I, Ohashi R, Nezu J, Sai Y, Kobayashi D, Oku A, Shimane M, and Tsuji A. Molecular and functional characterization of organic cation/carnitine transporter family in mice. J Biol Chem 275: 40064-40072, 2000.
42. Tamai I, Ohashi R, Nezu J, Yabuuchi H, Oku A, Shimane M, Sai Y, and Tsuji A. Molecular and functional identification of sodium ion-dependent, high affinity human carnitine transporter OCTN2. J Biol Chem 273: 20378-20382, 1998.
43. Tamai I, Yabuuchi H, Nezu J, Sai Y, Oku A, Shimane M, and Tsuji A. Cloning and characterization of a novel human pH-dependent organic cation transporter, 0CTN1. FEBS Lett 419:107-111, 1997.
44. Tein I, Bukovac SW, and Xie ZW. Characterization of the human plasmalemmal carnitine transporter in cultured skin fibroblasts. Biochem Biophys Res Commun 329: 145-155, 1996.
45. Tein I, Dimauro S, Xie-W, and DeVivo DC. Heterozygotes for plasmalemmal carnitine transporter defect are at increased risk for valproic acid-associated impairment of carnitine uptake in cultured human skin fibroblasts. J Inherit Metab Dis 18: 313-322, 1995.
46. Tein I, DiMauro S, Xie ZW, and DeVivo DC. Valproic acid impairs carnitine uptake in cultured human skin fibroblasts. An in vitro model for the pathogenesis of valproic acid-associated carnitine deficiency. Pediatr Res 34: 281-287, 1996.
47. Tein I and Xie ZW. Reversal of valproic acid-associated impairment of carnitine uptake in cultured human skin fibroblasts. Biochem Biophys Res Commun 204: 753-758, 1994.
48. +-coupled dipeptide (glycylsarcosine) transport across apical and basal borders of human intestinal Caco-2 cell monolayer display distinctive characteristics. Biochim Biophys Acta 1151: 237-245, 1993.
49. Vachon PH and Beaulieu JF. Transient mosaic patterns of morphological and functional differentiated in the Caco-2 cell line. Gastroenterology 103: 414-423, 1992.
50. Vaz FM, Scholte HR, Ruiter J, Hussaarts-Odijk LM, Pereira RR, Schweitzer S, de Klerk JB, Waterham HR, and Wanders RJ. Identification of two novel mutations in OCTN2 of three patients with systemic carnitine deficiency, Hum Genet 105: 157-161, 1999.
51. Verrotti A, Greco R, Morgese G, and Chiarelli F. Carnitine deficiency and hyperammonemia in children receiving valproic acid with and without other anticonvulsant drugs. Int J Clin Lab Res 29: 36-40, 1999.
52. Watanabe K, Sawano T, Endo T, Sakata M, and Sato J. Studies on intestinal absorption of sulpiride (2): transepithial transport of sulpiride across the human intestinal cell line Caco-2. Biol Pharm Bull 25: 1345-1350, 2002.
53. Watanabe K, Sawano T, Terada K, Endo T, Sakata M, and Sato J. Studies on intestinal absorption of sulpiride (1): carrier-mediated uptake of sulpiride in the human intestinal cell line Caco-2. Biol Pharm Bull 25: 885-890, 2002.
54. Wu X, George RL, Huang W, Wang H, Conway SJ, Leibach FH, and Ganapathy V. Structural and functional characteristics and tissue distribution pattern of rat OCTN1, an organic cation transporter, cloned from placenta. Biochim Biophys Acta 1466: 315-327, 2000.
55. Wu X, Huang W, Prasad PD, Seth P, Rajan DP, Leibach FH, Chen J, Conway SJ, and Ganapathy V. Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter. J Pharmacol Exp Ther 290: 1482-1492, 1999.
56. Wu X, Prasad PD, Leibach FH, and Ganapathy V. cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family. Biochem Biophys Res Commun 246: 589-595, 1998.
57. Yabuuchi H, Tamai I, Nezu J, Sakamoto K, Oku A, Shimane M, Sai Y, and Tsuji A. Novel membrane transporter OCTN1 mediates multispecific, bi-directional, and pH-dependent transport of organic cations. J Pharmacol Exp Ther 289: 768-773, 1999.
58. Yokogawa K, Miya K, Tamai I, Higashi Y, Nomura M, Miyamoto KI, and Tsuji A. Characteristics of L-carnitine transport in cultured human hepatoma HLF cells. J Pharm Pharmacol 51: 935-940, 1999.