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We carried out domain swaps followed by mutagenesis to locate the residues that prevent a crustacean Ultrabithorax protein from repressing limbs and allow Drosophila Ultrabithorax to repress limbs in Drosophila embryos. Coupled with comparative analysis of Ultrabiothorax protein sequence from a variety of insects and other arthropods, the results indicated that serine and threonine residues in consensus phosphorylation sites were mutated during the evolution of insects from crustaceans. This eventually resulted in a version of Ultrabithorax protein that repressed limbs in the abdomen of proto-insects, contributing to the formation of the hexapod body plan.
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Ronshaugen M., McGinnis N., McGinnis W. Hox protein mutation and macroevolution of the insect body plan. Nature. 415:2002;914-917 We carried out domain swaps followed by mutagenesis to locate the residues that prevent a crustacean Ultrabithorax protein from repressing limbs and allow Drosophila Ultrabithorax to repress limbs in Drosophila embryos. Coupled with comparative analysis of Ultrabiothorax protein sequence from a variety of insects and other arthropods, the results indicated that serine and threonine residues in consensus phosphorylation sites were mutated during the evolution of insects from crustaceans. This eventually resulted in a version of Ultrabithorax protein that repressed limbs in the abdomen of proto-insects, contributing to the formation of the hexapod body plan.
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Ronshaugen, M.1
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The authors mapped the domain differences that distinguish the functions of an onychophoran version of Ultrabithorax protein, which does not repress limbs in Drosophila embryos, from Drosophila Ultrabithorax, which does repress limbs. A C-terminal fragment of Drosophila Ultrabithorax, which contains a Gln/Ala-rich motif, was found to have a repressive function in cell culture assays, and was also found to confer a limb-repressive function when appended to the C-terminus of onychophoran Ultrabithorax. Although the deletion of this domain from Drosophila Ultrabithorax appears to have a minor influence on its limb-repression abilities, the study suggests that a repression domain evolved in insect Ultrabithorax that contributes to the suppression of limbs in hexapods.
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Galant R., Carroll S.B. Evolution of a transcriptional repression domain in an insect Hox protein. Nature. 415:2002;910-913 The authors mapped the domain differences that distinguish the functions of an onychophoran version of Ultrabithorax protein, which does not repress limbs in Drosophila embryos, from Drosophila Ultrabithorax, which does repress limbs. A C-terminal fragment of Drosophila Ultrabithorax, which contains a Gln/Ala-rich motif, was found to have a repressive function in cell culture assays, and was also found to confer a limb-repressive function when appended to the C-terminus of onychophoran Ultrabithorax. Although the deletion of this domain from Drosophila Ultrabithorax appears to have a minor influence on its limb-repression abilities, the study suggests that a repression domain evolved in insect Ultrabithorax that contributes to the suppression of limbs in hexapods.
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Galant, R.1
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A strategy for mapping bicoid on the phylogenetic tree
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A search was conducted for a bcd gene in the Tribolium Hox cluster at the conserved position between zen and Dfd/Hox4. The authors propose that bcd was acquired after the radiation of holometabolous insects and is characteristic of more derived insects. They also propose that bcd hijacked the function of an existing anterior determinant.
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Brown S., Fellers J., Shippy T., Denell R., Stauber M., Schmidt-Ott U. A strategy for mapping bicoid on the phylogenetic tree. Curr. Biol. 11:2001;R43-R44 A search was conducted for a bcd gene in the Tribolium Hox cluster at the conserved position between zen and Dfd/Hox4. The authors propose that bcd was acquired after the radiation of holometabolous insects and is characteristic of more derived insects. They also propose that bcd hijacked the function of an existing anterior determinant.
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The authors performed functional tests of Ftz orthologues of Drosophila and Schistocerca using overexpression assays in Drosophila embryos, assaying the activation or repression of target genes involved in CNS and segmentation functions. They concluded that Schistocerca Ftz closely mimicked its Drosophila orthologue in the regulation of CNS target genes but not in the regulation of segmentation target genes.
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Alonso C.R., Maxton-Kuechenmeister J., Akam M. Evolution of Ftz protein function in insects. Curr. Biol. 11:2001;1473-1478 The authors performed functional tests of Ftz orthologues of Drosophila and Schistocerca using overexpression assays in Drosophila embryos, assaying the activation or repression of target genes involved in CNS and segmentation functions. They concluded that Schistocerca Ftz closely mimicked its Drosophila orthologue in the regulation of CNS target genes but not in the regulation of segmentation target genes.
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Veraksa A., Del Campo M., McGinnis W. Developmental patterning genes and their conserved functions: from model organisms to humans. Mol. Genet. Metab. 69:2000;85-100.
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Hanks M.C., Loomis C.A., Harris E., Tong C.-X., Anson-Cartwright L., Auerbach A., Joyner A. Drosophila engrailed can substitute for mouse Engrailed 1 function in mid-hindbrain, but not limb development. Development. 125:1998;4521-4530.
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The Drosophila Hox gene Deformed sculpts head morphology via direct regulation of the apoptosis activator reaper
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The Hox protein Deformed directly activates the apoptosis-promoting gene reaper to maintain a segment boundary in the head of Drosophila embryos. Surprisingly, expression of reaper alone at the boundary in a Deformed mutant embryo is capable of rescuing the segmentation defect.
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Lohmann I., McGinnis N., Bodmer M., McGinnis W. The Drosophila Hox gene Deformed sculpts head morphology via direct regulation of the apoptosis activator reaper. Cell. 110:2002;457-466 The Hox protein Deformed directly activates the apoptosis-promoting gene reaper to maintain a segment boundary in the head of Drosophila embryos. Surprisingly, expression of reaper alone at the boundary in a Deformed mutant embryo is capable of rescuing the segmentation defect.
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Abdominal A specifies one cell type in Drosophila by regulating one principal target gene
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In a Drosophila chordotonal organ precursor cell, the Hox protein Abdominal-A activates the expression of the rhomboid gene, which promotes the secretion of an active form of Spitz, a ligand of the epidermal growth factor receptor. The Spitz signal is required for the differentiation of adjacent cells called oenocytes. Surprisingly, expression of rhomboid alone in chordotonal precursor cells of abdominal-A mutant embryos is capable of rescuing the oenocyte defect.
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Brodu V., Elstob P.R., Gould A.P. abdominal A specifies one cell type in Drosophila by regulating one principal target gene. Development. 129:2002;2957-2963 In a Drosophila chordotonal organ precursor cell, the Hox protein Abdominal-A activates the expression of the rhomboid gene, which promotes the secretion of an active form of Spitz, a ligand of the epidermal growth factor receptor. The Spitz signal is required for the differentiation of adjacent cells called oenocytes. Surprisingly, expression of rhomboid alone in chordotonal precursor cells of abdominal-A mutant embryos is capable of rescuing the oenocyte defect.
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A single Hox3 gene with composite bicoid and zerknüllt expression characteristics in non-Cyclorrhaphan flies
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This paper covers the characterization of the maternal and zygotic expression patterns of Hox3/zen orthologues from three basal flies. The authors propose a model whereby a Hox3/zen/bcd ancestor in insects underwent duplication and one gene evolved a zygotic function in extra-embryonic tissue (zen) whereas the other evolved a maternal function as an anterior determinant (bcd).
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Stauber M., Prell A., Schmidt-Ott U. A single Hox3 gene with composite bicoid and zerknüllt expression characteristics in non-Cyclorrhaphan flies. Proc. Natl. Acad. Sci. USA. 99:2002;274-279 This paper covers the characterization of the maternal and zygotic expression patterns of Hox3/zen orthologues from three basal flies. The authors propose a model whereby a Hox3/zen/bcd ancestor in insects underwent duplication and one gene evolved a zygotic function in extra-embryonic tissue (zen) whereas the other evolved a maternal function as an anterior determinant (bcd).
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Brown S.J., Fellers J.P., Shippy T.D., Richardson E.A., Maxwell M., Stuart J.J., Denell R.E. Sequence of the Tribolium castaneum homeotic complex: the region corresponding to the Drosophila melanogaster antennapedia complex. Genetics. 160:2002;1067-1074.
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Characterization of the expression patterns of all the genes from the Hox cluster of the centipede Lithobius atkinsoni. The paper also includes a fairly comprehensive comparison of all arthropod Hox gene expression patterns and discusses the implications for ftz and Hox3/zen evolution.
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Hughes C.L., Kaufman T.C. Exploring the myriapod body plan: expression patterns of the ten Hox genes in a centipede. Development. 129:2002;1225-1238 Characterization of the expression patterns of all the genes from the Hox cluster of the centipede Lithobius atkinsoni. The paper also includes a fairly comprehensive comparison of all arthropod Hox gene expression patterns and discusses the implications for ftz and Hox3/zen evolution.
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The nuclear hormone receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz
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Yu Y., Li W., Su K., Yussa M., Han W., Perrimon N., Pick L. The nuclear hormone receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz. Nature. 385:1997;552-555.
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Nature
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Yu, Y.1
Li, W.2
Su, K.3
Yussa, M.4
Han, W.5
Perrimon, N.6
Pick, L.7
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42
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0031026823
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The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors
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Guichet A., Copeland J.W.R., Erdelyi M., Hlousek D., Zavorszky P., Ho J., Brown S., Percival-Smith A., Krause H.M., Ephrussi A. The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors. Nature. 385:1997;548-552.
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Nature
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Guichet, A.1
Copeland, J.W.R.2
Erdelyi, M.3
Hlousek, D.4
Zavorszky, P.5
Ho, J.6
Brown, S.7
Percival-Smith, A.8
Krause, H.M.9
Ephrussi, A.10
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43
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0035253854
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FTZ-factor1 and Fushi tarazu interact via conserved nuclear receptor and coactivator motifs
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The Ftz-F1 and Ftz proteins interact via an LXXLL motif and homeodomain residues in the Ftz protein. The LXXLL motif was originally defined as a sequence required for interactions between hormone receptors and their cofactors.
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Schwartz C.J.E., Sampson H.M., Hlousek D., Percival-Smith A., Copeland J.W.R., Simmonds A.J., Krause H.M. FTZ-factor1 and Fushi tarazu interact via conserved nuclear receptor and coactivator motifs. EMBO J. 20:2001;510-519 The Ftz-F1 and Ftz proteins interact via an LXXLL motif and homeodomain residues in the Ftz protein. The LXXLL motif was originally defined as a sequence required for interactions between hormone receptors and their cofactors.
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EMBO J.
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Schwartz, C.J.E.1
Sampson, H.M.2
Hlousek, D.3
Percival-Smith, A.4
Copeland, J.W.R.5
Simmonds, A.J.6
Krause, H.M.7
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44
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0034886072
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The nuclear receptor Ftz-F1 and homeodomain protein Ftz interact through evolutionarily conserved protein domains
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•], these authors defined the Ftz LXXLL motif and the Ftz homeodomain as the principal interaction surfaces with its cofactor Ftz-F1, an orphan hormone receptor. This paper also shows that a mouse homologue of Ftz-F1, called SF-1, can rescue the segmentation defects of Drosophila Ftz-F1 mutants.
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•], these authors defined the Ftz LXXLL motif and the Ftz homeodomain as the principal interaction surfaces with its cofactor Ftz-F1, an orphan hormone receptor. This paper also shows that a mouse homologue of Ftz-F1, called SF-1, can rescue the segmentation defects of Drosophila Ftz-F1 mutants.
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Mech. Dev.
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Yussa, M.1
Lohr, U.2
Su, K.3
Pick, L.4
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45
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0036080021
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Exploring myriapod segmentation: The expression patterns of even-skipped, engrailed, and wingless in a centipede
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In centipede embryos, the even-skipped orthologue is expressed in patterns that suggest the gene has segment boundary functions as well as posterior segment identity functions.
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Hughes C.L., Kaufman T.C. Exploring myriapod segmentation: the expression patterns of even-skipped, engrailed, and wingless in a centipede. Dev. Biol. 247:2002;47-61 In centipede embryos, the even-skipped orthologue is expressed in patterns that suggest the gene has segment boundary functions as well as posterior segment identity functions.
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Dev. Biol.
, vol.247
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Hughes, C.L.1
Kaufman, T.C.2
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46
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0035884333
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Amphioxus Evx genes: Implications for the evolution of the midbrain-hindbrain boundary and the chordate tailbud
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Ferrier D.E.K., Minguillon C., Cebrian C., Garcia-Fernandez J. Amphioxus Evx genes: implications for the evolution of the midbrain-hindbrain boundary and the chordate tailbud. Dev. Biol. 237:2001;270-281.
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Dev. Biol.
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Ferrier, D.E.K.1
Minguillon, C.2
Cebrian, C.3
Garcia-Fernandez, J.4
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47
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0030731640
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Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning
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Gavalas A., Davenne M., Lumsden A., Chambon P., Rijli F.M. Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning. Development. 124:1997;3693-3702.
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Development
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Gavalas, A.1
Davenne, M.2
Lumsden, A.3
Chambon, P.4
Rijli, F.M.5
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48
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0036677546
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Evolving role of Antennapedia protein in arthropod limb patterning
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Shiga et al. found that the altered morphology of the first thoracic limb appendage in the crustacean Daphnia correlates with altered patterns of Distal-less and Antennapedia in the limb primordium. In Drosophila embryos, Daphnia Antennapedia protein, unlike its Drosophila homologue, has a repressive effect on Distal-less transcription, and the protein region conferring this activity was mapped to the N-terminal region of the protein. Their results suggest that Daphnia Antennapedia diversifies anterior leg morphology by repressing Distal-less, and that this repressive function evolved after the crustaceans and insects split.
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Shiga Y., Yasumoto R., Yamagata H., Hayashi S. Evolving role of Antennapedia protein in arthropod limb patterning. Development. 129:2002;3555-3561 Shiga et al. found that the altered morphology of the first thoracic limb appendage in the crustacean Daphnia correlates with altered patterns of Distal-less and Antennapedia in the limb primordium. In Drosophila embryos, Daphnia Antennapedia protein, unlike its Drosophila homologue, has a repressive effect on Distal-less transcription, and the protein region conferring this activity was mapped to the N-terminal region of the protein. Their results suggest that Daphnia Antennapedia diversifies anterior leg morphology by repressing Distal-less, and that this repressive function evolved after the crustaceans and insects split.
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(2002)
Development
, vol.129
, pp. 3555-3561
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Shiga, Y.1
Yasumoto, R.2
Yamagata, H.3
Hayashi, S.4
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