Selective plasma membrane permeabilization by freeze-thawing and immunofluorescence epitope access to determine the topology of intracellular membrane proteins

Journal of Immunological Methods

Volumn 275, Issue 1-2, 2003, Pages 169-177

  Mardones, Gonzalo ^a   González, Alfonso ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

Epitope accessibility; Freeze thawing; Immunofluorescence; Membrane protein topology; Semi permeabilization

Indexed keywords

ANTIBODY; EPITOPE; MEMBRANE PROTEIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; EPITHELIUM CELL; FEMALE; FIBROBLAST; GOLGI COMPLEX; HELA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; INTRACELLULAR MEMBRANE; LYSOSOME; NONHUMAN; PEROXISOME; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; THAWING;

EID: 0037377019     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00015-2     Document Type: Article

References (53)

1. Acharya U., McCaffery J.M., Jacobs R., Malhotra V. Reconstitution of vesiculated Golgi membranes into stacks of cisternae: requirement of NSF in stack formation. J. Cell Biol. 129:1995;577.
2. Ahle S., Mann A., Eichelsbacher U., Ungewickell E. Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane. EMBO J. 7:1988;919.
3. Ahnert-Hilger G., Bhakdi S., Gratzl M. Minimal requirements for exocytosis. A study using PC 12 cells permeabilized with staphylococcal α-toxin. J. Biol. Chem. 260:1985;12730.
4. Biemesderfer D., DeGray B., Aronson P.S. Membrane topology of NHE3. Epitopes within the carboxyl-terminal hydrophilic domain are exoplasmic. J. Biol. Chem. 273:1998;12391.
5. Cereijido M., Robbins E.S., Dolan W.J., Rotunno C.A., Sabatini D.D. Polarized monolayers formed by epithelial cells on a permeable and translucent support. J. Cell Biol. 77:1978;853.
6. Cloke J.D., Davis E.A., Gordon J., Hsieh S.I., Grider J., Addis P.B., McGrath C.J. Scanning and transmission electron microscopy of normal and PSE porcine muscle. Scanning Electron Microsc. Pt. 3:1981;435.
7. De Lemos-Chiarandini C., Frey A.B., Sabatini D.D., Kreibich G. Determination of the membrane topology of the phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4 using site-specific antibodies. J. Cell Biol. 104:1987;209.
8. Diaz R., Stahl P.D. Digitonin permeabilization procedures for the study of endosome acidification and function. Methods Cell Biol. 31:1989;25.
9. Du X., Stoops J.D., Mertz J.R., Stanley C.M., Dixon J.L. Identification of two regions in apolipoprotein B100 that are exposed on the cytosolic side of the endoplasmic reticulum membrane. J. Cell Biol. 141:1998;585.
10. Duden R., Griffiths G., Frank R., Argos P., Kreis T.E. β-COP, a 110 kD protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to β-adaptin. Cell. 64:1991;649.
11. Eckhardt M., Gotza B., Gerardy-Schahn R. Membrane topology of the mammalian CMP-sialic acid transporter. J. Biol. Chem. 274:1999;8779.
12. Faúndez V., Krauss R., Holuigue L., Garrido J., González A. Epidermal growth factor receptor in synaptic fractions of the rat central nervous system. J. Biol. Chem. 267:1992;20363.
13. Fukuda M., Viitala J., Matteson J., Carlsson S.R. Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences. J. Biol. Chem. 263:1988;18920.
14. Gleeson P.A., Anderson T.J., Stow J.L., Griffiths G., Toh B.H., Matheson F. p230 is associated with vesicles budding from the trans-Golgi network. J. Cell Sci. 109:1996;2811.
15. Gulevsky A.K., Sakharov B.V., Volkov V. Role of dehydration in changing the permeability of erythrocyte plasma membranes by freeze-thawing. Biochim. Biophys. Acta. 728:1983;371.
16. Hanada K., Hara T., Nishijima M., Kuge O., Dickson R.C., Nagiec M.M. A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis. J. Biol. Chem. 272:1997;32108.
17. Harano T., Shimizu N., Otera H., Fujiki Y. Transmembrane topology of the peroxin, Pex2p, an essential component for the peroxisome assembly. J. Biochem. (Tokyo). 125:1999;1168.
18. 2+ and guanine nucleotides in exocytotic secretion from permeabilized rat mast cells. J. Cell Biol. 105:1987;191.
19. Kaetzel C.S., Rao C.K., Lamm M.E. Protein disulphide-isomerase from human placenta and rat liver: purification and immunological characterization with monoclonal antibodies. Biochem. J. 241:1987;39.
20. 2+-stimulatable and protein kinase C-inhibitable accumulation of inositol 1,3,4,6-tetrakisphosphate in human platelets. Biochem. J. 270:1990;125.
21. Kooy J., Toh B.H., Pettitt J.M., Erlich R., Gleeson P.A. Human autoantibodies as reagents to conserved Golgi components. Characterization of a peripheral, 230-kDa compartment-specific Golgi protein. J. Biol. Chem. 267:1992;20255.
22. Kris R.M., Lax I., Gullick W., Waterfield M.D., Ullrich A., Fridkin M., Schlessinger J. Antibodies against a synthetic peptide as a probe for the kinase activity of the avian EGF receptor and v-erbB protein. Cell. 40:1985;619.
23. 5 in the endoplasmic reticulum membrane. J. Biochem. (Tokyo). 120:1996;828.
24. Lammers M., Follmann H. Deoxyribonucleotide biosynthesis in yeast: assay and properties of ribonucleotide reductase in permeabilized Saccharomyces cerevisiae cells. Arch. Biochem. Biophys. 244:1986;430.
25. Levy S., Nguyen V.Q., Andria M.L., Takahashi S. Structure and membrane topology of TAPA-1. J. Biol. Chem. 266:1991;14597.
26. Lovelock J.E. The haemolysis of human red blood-cells by freezing and thawing. Biochim. Biophys. Acta. 10:1953;414.
27. Luyet B.J., Gehenio P.M. The mechanism of injury and death by low temperature. Biodynamica. 3:1940;33.
28. Luzio J.P., Brake B., Banting G., Howell K.E., Braghetta P., Stanley K.K. Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38). Biochem. J. 270:1990;97.
29. Malik S., Hisatake K., Sumimoto H., Horikoshi M., Roeder R.G. Sequence of general transcription factor TFIIB and relationships to other initiation factors. Proc. Natl. Acad. Sci. U. S. A. 88:1991;9553.
30. Mazur P., Leibo S.P., Chu E.H. A two-factor hypothesis of freezing injury. Evidence from Chinese hamster tissue-culture cells. Exp. Cell Res. 71:1972;345.
31. Meryman H.T. Modified model for the mechanism of freezing injury in erythrocytes. Nature. 218:1968;333.
32. 2+-ATPase with sequence-specific antibodies. Evidence for plasticity of the c-terminal domain. J. Biol. Chem. 272:1997;29015.
33. Moremen K.W., Robbins P.W. Isolation, characterization, and expression of cDNAs encoding murine α-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans. J. Cell Biol. 115:1991;1521.
34. Morikawa S., Harada T. Immunohistochemical localization of catalase in mammalian tissues. J. Histochem. Cytochem. 17:1969;30.
35. Mu F.T., Callaghan J.M., Steele-Mortimer O., Stenmark H., Parton R.G., Campbell P.L., McCluskey J., Yeo J.P., Tock E.P., Toh B.H. EEA1, an early endosome-associated protein. EEA1 is a conserved α-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. J. Biol. Chem. 270:1995;13503.
36. Mura U., Stadtman E.R. Glutamine synthetase adenylylation in permeabilized cells of Escherichia coli. J. Biol. Chem. 256:1981;13014.
37. Nakamura N., Rabouille C., Watson R., Nilsson T., Hui N., Slusarewicz P., Kreis T.E., Warren G. Characterization of a cis-Golgi matrix protein, GM130. J. Cell Biol. 131:1995;1715.
38. Narula N., McMorrow I., Plopper G., Doherty J., Matlin K.S., Burke B., Stow J.L. Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes. J. Cell Biol. 117:1992;27.
39. Nathke I.S., Heuser J., Lupas A., Stock J., Turck C.W., Brodsky F.M. Folding and trimerization of clathrin subunits at the triskelion hub. Cell. 68:1992;899.
40. Robinson M.S. 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J. Cell Biol. 104:1987;887.
41. Robinson M.S. Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (α-adaptins). J. Cell Biol. 108:1989;833.
42. Robinson M.S. Cloning and expression of γ-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus. J. Cell Biol. 111:1990;2319.
43. Robinson M.S., Kreis T.E. Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators. Cell. 69:1992;129.
44. Salazar G., González A. Novel mechanism for regulation of epidermal growth factor receptor endocytosis revealed by protein kinase A inhibition. Mol. Biol. Cell. 13:2002;1677.
45. Santos M.J., Imanaka T., Shio H., Small G.M., Lazarow P.B. Peroxisomal membrane ghosts in Zellweger syndrome-aberrant organelle assembly. Science. 239:1988;1536.
46. Sato J.D., Kawamoto T., Le A.D., Mendelsohn J., Polikoff J., Sato G.H. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1:1983;511.
47. Schlenstedt G., Hurt E., Doye V., Silver P.A. Reconstitution of nuclear protein transport with semi-intact yeast cells. J. Cell Biol. 123:1993;785.
48. Shiloh H., Iancu T.C., Sheinfeld M., Kraiem Z. The influence of cryopreservation on the ultrastructural morphology of human thyroid cells. Cryobiology. 24:1987;303.
49. Song W.X., Vacca M.F., Welti R., Rintoul D.A. Effects of gangliosides GM3 and De-N-acetyl GM3 on epidermal growth factor receptor kinase activity and cell growth. J. Biol. Chem. 266:1991;10174.
50. van Zeeland A.A., Smith C.A., Hanawalt P.C. Sensitive determination of pyrimidine dimers in DNA of UV-irradiated mammalian cells. Introduction of T4 endonuclease V into frozen and thawed cells. Mutat. Res. 82:1981;173.
51. Weber K., Pollack R., Bibring T. Antibody against tubulin: the specific visualization of cytoplasmic microtubules in tissue culture cells. Proc. Natl. Acad. Sci. U. S. A. 72:1975;459.
52. Wilkinson J., Higgins J.A., Groot P.H., Gherardi E., Bowyer D.E. Membrane-bound apolipoprotein B is exposed at the cytosolic surface of liver microsomes. FEBS Lett. 304:1992;24.
53. Zurzolo C., Gentile R., Mascia A., Garbi C., Polistina C., Aloj L., Avvedimento V.E., Nitsch L. The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines. J. Cell Sci. 98:1991;65.