In situ topology of cytochrome b5 in the endoplasmic reticulum membrane

Journal of Biochemistry

Volumn 120, Issue 4, 1996, Pages 828-833

  Kuroda, Rieko ^a   Kinoshita, Jun Ya ^a   Honsho, Masanori ^a   Mitoma, Jun Ya ^a   Ito, Akio ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Cytochrome b5; Endoplasmic reticulum; Immunofluorescence microscopy; Membrane topology; Tail anchored protein

Indexed keywords

CYTOCHROME B5; MONOCLONAL ANTIBODY; MYC PROTEIN; PROTEIN DISULFIDE ISOMERASE; STREPTOLYSIN O; TRITON X 100; TYLOXAPOL;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MEMBRANE STRUCTURE; NONHUMAN;

ANIMALIA;

EID: 0029807380     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021486     Document Type: Article

References (28)

1. 5 from liver microsomes. J. Biol. Chem. 243, 4922-4923
2. 5 that contains an additional Hydrophobic sequence of 40 amino acid residues. Proc. Natl. Acad. Sci. USA 68, 1042-1046
3. Ozols, J. and Gerard, C. (1977) Covalent structure of the membranous segment of horse cytochrome 65. J. Biol. Chem. 252, 8549-8553
4. 5 are necessary for its targeting to the endoplasmic reticulum. EMBO J. 11, 4197-4203
5. 5 reductase, cytochrome P-450 reductase and epoxide hydrolase. Eur. J. Biochem. 122, 393-402
6. Sakaguchi, M., Mihara, K., and Sato, R. (1984) Signal recognition particle is required for co-translational insertion of cytochrome P-450 into microsomal membranes. Proc. Natl. Acad. Sci. USA 81, 3361-3364
7. Anderson, D.J., Mostov, K.E., and Blobel, G. (1983) Mechanisms of integration of de novo-synthesized polypeptides into membranes: Signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome 65. Proc. Natl. Acad. Sci. USA 80, 7249-7253
8. 5. Eur. J. Biochem. 123, 121-126
9. Ozols, J. (1989) Structure of cytochrome 66 and its topology in the microsomal membrane. Biochim. Biophys. Acta 997, 121-130
10. 5 spans the bilayer of the endoplasmic reticulum. J. Biol. Chem. 270, 3414-3422
11. Kunkel, T.A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488-492
12. Watt, R., Stanton, L.W., Marcu, K.B., Gallo, R.C., Croce, C.M., and Rovera, G. (1983) Nucleotide sequence of cloned cDNA of human c-myc oncogene. Nature 303, 725-728
13. Ito, A., Miyazoe, R., Mitoma, J.Y., Akao, T. Osaki, T., and Kunitake, T. (1990) Synthetic cationic amphiphiles for liposome-mediated DNA transfection. Biochem. Int. 22, 235-241
14. αfluorenylmethoxycarbonyl amino acids on polyamide supports: Synthesis of substance P and of acyl carrier protein 65-74 decapeptide, J. Chem. Soc. Perkin. Transe. I 1, 538-546
15. Liu, F.T., Zinnecker, M., Hamaoka, T., and Katz, D.H. (1979) New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates. Biochemistry 18, 690-697
16. Otto, J.C. and Smith, W.L. (1994) The orientation of prostaglandin endoperoxide syntheses-1 and -2 in the endoplasmic reticulum. J. Biol. Chem. 28B, 19868-19875
17. 5 in yeast and characterization of mutants of the membrane-anchoring domain. J. Biol. Chem. 267, 12583-12591
18. 2 termini of the membrane-binding segment of cytochrome it on the same side of phospholipid bilayers. J. Biol. Chem. 256, 3951-3955
19. 5 as studied by cross-linking with photoactivatable phospholipids. J. Biol. Chem. 258, 9136-9142
20. 5 tightly bound to dimyristoylphoephatidylcholine vesicles. J. Biol. Chem. 262, 15563-15567
21. 5 to liver microsomes. J. Biol. Chem. 247, 7188-7194
22. 5 into liver microsomal membranes. Biochem. Biophys. Res. Commun. 51, 1-7
23. 5 to membranes of isolation subcellular organelles from rat liver. J. Cell. Biol. 79, 291-313
24. Sabatini, D.D., Kreibich, G., Morimoto, T., and Adesnik, M. (1982) Mechanisms for the incorporation of proteins in membranes and organelles. J. Cell. Biol. 92, 1-22
25. Andrews, D.W., Lauffer, L., Walter, P., and Lingappa, V.R. (1989) Evidence for a two-step mechanism involved in assembly of functional signal recognition particle receptor. J. Cell. Biol. 108, 797-810
26. Miyauchi, K., Masaki, R., Taketani, S., Yamamoto, A., Akayama, M., and Tashiro, Y. (1991) Molecular cloning, sequencing, and expression of cDNA for rat liver microsomal aldehyde dehydrogenase. J. Biol. Chem. 266, 19536-19542
27. Masaki, R., Yamamoto, A., and Tashiro, Y. (1994) Microsomal aldehyde dehydrogenase is localized to the endoplasmic reticulum via its carboxy-terminal 35 amino acids. J. Cell Biol. 126, 1407-1420
28. Kutay, U., Ahnert-Hilger, G., Hartmann, E., Wiedenmann, B., and Rapoport, T.A. (1995) Transport route for synatobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane. EMBO J. 14, 217-223