Structural model for 12-helix transporters belonging to the major facilitator superfamily

Journal of Bacteriology

Volumn 185, Issue 5, 2003, Pages 1712-1718

  Hirai, Teruhisa ^a   Heymann, Jürgen A W ^a   Maloney, Peter C ^b   Subramaniam, Sriram ^a,c  

^a NATIONAL CANCER INSTITUTE   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE PROTEIN; OXALIC ACID;

ARTICLE; BACTERIAL MEMBRANE; CYTOPLASM; ION TRANSPORT; MEMBRANE TRANSPORT; MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; SOLUTE; THREE DIMENSIONAL IMAGING;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARRIER PROTEINS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MODELS, MOLECULAR; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURAL HOMOLOGY, PROTEIN;

BACTERIA (MICROORGANISMS);

EID: 0037373068     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.5.1712-1718.2003     Document Type: Article

References (32)

1. Frillingos, S., M. Sahin-Toth, J. Wu, and H. R. Kaback. 1998. Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins. FASEB J. 12:1281-1299.
2. Goswitz, V. C., and R. J. Brooker. 1995. Structural features of the uniporter/symporter/antiporter superfamily. Protein Sci. 4:534-537.
3. Green, A. L., E. J. Anderson, and R. J. Brooker. 2000. A revised model for the structure and function of the lactose permease. Evidence that a face on transmembrane segment 2 is important for conformational changes. J. Biol. Chem. 275:23240-23246.
4. Guan, L., A. B. Weinglass, and H. R. Kaback. 2001. Helix packing in the lactose permease of Escherichia coli: localization of helix VI. J. Mol. Biol. 312:69-77.
5. He, M. M., J. Voss, W. L. Hubbell, and H. R. Kaback. 1997. Arginine 302 (helix IX) in the lactose permease of Escherichia coli is in close proximity to glutamate 269 (helix VIII) as well as glutamate 325. Biochemistry 36:13682-13687.
6. He, M. M., J. Voss, W. L. Hubbell, and H. R. Kaback. 1995. Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli. 2. Proximity of helix IX (Arg302) with helix X (His322 and Glu325). Biochemistry 34:15667-15670.
7. Henderson, P. J., and M. C. Maiden. 1990. Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes. Phil. Trans. R. Soc. Lond. B Biol. Sci. 326:391-410.
8. Heymann, J. A., R. Sarker, T. Hirai, D. Shi, J. L. Milne, P. C. Maloney, and S. Subrarnaniam. 2001. Projection structure and molecular architecture of OxlT, a bacterial membrane transporter. EMBO J. 20:4408-4413.
9. Hirai, T., J. A. Heymann, D. Shi, R. Sarker, P. C. Maloney, and S. Subramaniam. 2002. Three-dimensional structure of a bacterial oxalate transporter. Nat. Struct. Biol. 9:597-600.
10. Hruz, P. W., and M. M. Mueckler. 2001. Structural analysis of the GLUT1 facilitative glucose transporter. Mol. Membr, Biol. 18:183-193.
11. Jessen-Marshall, A. E., and R. J. Brooker. 1996. Evidence that transmembrane segment 2 of the lactose permease is part of a conformationally sensitive interface between the two halves of the protein. J. Biol. Chem. 271:1400-1404.
12. Jessen-Marshall, A. E., N. J. Paul, and R. J. Brooker. 1995. The conserved motif, GXXX(D/E)(R/K)XG[X](R/K)(R/K), in hydrophilic loop 2/3 of the lactose permease. J. Biol. Chem. 270:16251-16257.
13. Jung, K., J. Voss, M. He, W. L. Hubbell, and H. R. Kaback. 1995. Engineering a metal binding site within a polytopic membrane protein, the lactose permease of Escherichia coli. Biochemistry 34:6272-6277.
14. Kaback, H. R., M. Sahin-Toth, and A. B. Weinglass. 2001. The kamikaze approach to membrane transport. Nat. Rev. Mol. Cell. Biol. 2:610-620.
15. Kim, Y. M., L. Ye, and P. C. Maloney. 2001. Helix proximity in OxlT, the oalate: formate antiporter of Oxalobacter formigenes. Cross-linking between TM2 and TM11. J. Biol. Chem. 276:36681-36686.
16. King, S. C., C. L. Hansen, and T. H. Wilson. 1991. The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli. Biochim. Biophys. Acta 1062:177-186.
17. Lee, J. I., P. P. Hwang, C. Hansen, and T. H. Wilson. 1992. Possible salt bridges between transmembrane alpha-helices of the lactose carrier of Escherichia coli. J. Biol. Chem. 267:20758-20764.
18. Pao, S. S., I. T. Paulsen, and M. H. Saier, Jr. 1998. Major facilitator superfamily. Microbiol. Mol. Biol. Rev. 62:1-34.
19. Paulsen, I. T., L. Nguyen, M. K. Sliwinski, R. Rabus, and M. H. Saier, Jr. 2000. Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes. J. Mol. Biol. 301:75-100.
20. Rubin, R. A., S. B. Levy, R. L. Heinrikson, and F. J. Kezdy. 1990. Gene duplication in the evolution of the two complementing domains of gramnegative bacterial tetracycline efflux proteins. Gene. 87:7-13.
21. + antiporter. J. Biol. Chem. 276:20330-20339.
22. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
23. Wang, Q., and H. R. Kaback. 1999. Location of helix III in the lactose permease of Escherichia coli as determined by site-directed thiol cross-linking. Biochemistry 38:16777-16782.
24. Wang, Q., and H. R. Kaback. 1999. Proximity relationships between helices I and XI or XII in the lactose permease of Escherichia coli determined by site-directed thiol cross-linking. J. Mol. Biol. 291:683-692.
25. Wu, J., D. Hardy, and H. R. Kaback. 1999. Site-directed chemical cross-linking demonstrates that helix IV is close to helices VII and XI in the lactose permease. Biochemistry 38:1715-1720.
26. Wu, J., D. Hardy, and H. R. Kaback. 1999. Tertiary contacts of helix V in the lactose permease determined by site-directed chemical cross-linking in situ. Biochemistry 38:2320-2325.
27. Wu, J., D. Hardy, and H. R. Kaback. 1998. Tilting of helix I and ligand-induced changes in the lactose permease determined by site-directed chemical cross-linking in situ. Biochemistry 37:15785-15790.
28. Wu, J., D. Hardy, and H. R. Kaback. 1998. Transmembrane helix tilting and ligand-induced conformational changes in the lactose permease determined by site-directed chemical crosslinking in situ. J. Mol. Biol. 282:959-967.
29. Wu, J., and H. R. Kaback. 1996. A general method for determining helix packing in membrane proteins in situ: helices I and II are close to helix VII in the lactose permease of Escherichia coli. Proc. Natl. Acad. Sci. USA 93:14498-14502.
30. Wu, J., and H. R. Kaback. 1997. Helix proximity and ligand-induced conformational changes in the lactose permease of Escherichia coli determined by site-directed chemical crosslinking. J. Mol. Biol. 270:285-293.
31. + antiporter of Escherichia coli encoded by transposon Tn10. The role of a conserved sequence motif, GXXXXRXGRR, in a putative cytoplasmic loop between helices 2 and 3. J. Biol. Chem. 267:19155-19162.
32. Zhao, M., K. C. Zen, W. L. Hubbell, and H. R. Kaback. 1999. Proximity between Glu126 and Arg144 in the lactose permease of Escherichia coli. Biochemistry 38:7407-7412.