Integration, amplification and expression of the Bacillus licheniformis α-amylase gene in Bacillus subtilis chromosome

Journal of Biotechnology

Volumn 8, Issue 1, 1988, Pages 23-38

  Declerck, Nathalie ^a   Joyet, Philippe ^a   Le Coq, Dominique ^a   Heslot, Henri ^a  

^a AGROPARISTECH   (France)

Author keywords

(B. subtilis); Amplifiable integrative vector; Expression; Stability; Thermostable amylase

Indexed keywords

BACTERIOLOGY; BIOCHEMISTRY; BIOTECHNOLOGY; ENZYMES;

AMPLIFIABLE INTEGRATIVE VECTOR; BACILLUS LICHENIFORMIS ALPHA AMYLASE GENE; BACILLUS SUBTILIS CHROMOSOME; PLASMIDS; THERMOSTABLE Α-AMYLASE;

BIOLOGICAL MATERIALS;

BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS;

EID: 0024000744     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(88)90065-X     Document Type: Article

References (38)

1. Amplification of a chromosomal region in Bacillus subtilis
2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
3. Segregational instability of pUB110-derived recombinant plasmids in Bacillus subtilis
4. Supercoiled circular DNA-protein complex in E coli Purification and induced conversion to an open circular DNA form
5. Replication functions of pC194 are necessary for efficient plasmid transduction of phage M13
6. Expression of the staphylococcal protein A gene in Bacillus subtilis by integration of the intact gene into B. subtilis chromosome
7. Construction and properties of an integradable plasmid for Bacillus subtilis
8. Mapping of a gene under sporulation control by insertion of a drug resistance marker in B. subtilis chromosome
9. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance
10. Stable gene amplification in the chromosome of Bacillus subtilis
11. Cloning of a thermostable α-amylase gene from Bacillus licheniformis and its expression in E. coli and Bacillus subtilis
12. Expression of a thermostable α-amylase gene under the control of levansucrase inducible promoter from Bacillus subtilis
13. Investigation of plasmid instability in amylase-producing Bacillus subtilis using continuous culture
14. Enhancement of α-amylase production by integrating and amplifying the α-amylase gene of Bacillus amyloliquefaciens in the genome of B. subtilis
15. Generation of deletions in pneumococcal mal genes cloned in Bacillus subtilis
16. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400
17. Insertional mutagenesis: use in cloning of Bacillus subtilis genes
18. Insertional mutagenesis in Bacillus subtilis: mechanism and use in genetic cloning
19. Recombination occurs more often in plasmids than in the chromosome of Bacillus subtilis
20. Integration of linear, heterologous DNA molecules into the Bacillus subtilis chromosome: mechanism and use in induction of predictable rearrangements
21. Integradable α-amylase plasmid for generating random transcriptional fusions in Bacillus subtilis
22. Molecular cloning in Bacillus subtilis of a Bacillus licheniformis gene coding for a thermostable α-amylase
23. Isolation of plasmid deletion mutants and study of their instability
24. Insertion of eukaryotic DNA into Bacillus subtilis genome by means of temperature sensitive plasmid vector
25. Use of chromosomal integration in the establishment and expression of blaZ, a Staphylococcus aureus β-lactamase gene in Bacillus subtilis
26. Isolation and 5′-end nucleotide sequence of Bacillus licheniformis α-amylase gene
27. High expression of Bacillus licheniformis α-amylase with a Bacillus secretion vector
28. Detection of specific sequences among DNA fragments separated by gel electrophoresis
29. Extracellular production of cloned α-amylase by Escherichia coli
30. RecE independent deletion of recombinant plasmids in Bacillus subtilis
31. Stability of the recombinant plasmid carrying the Bacillus amyloliquefaciens α-amylase gene in B. subtilis chromosome
32. Integration of vector-containing Bacillus subtilis chromosomal DNA by a Campbell-like mechanism
33. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194
34. Growth defects of Escherichia coli cells which contain the gene of an α-amylase from Bacillus coagulans on a multicopy plasmid
35. The mechanism of insertion of a segment of heterologous DNA into the chromosome of Bacillus subtilis
36. Gene amplification in Bacillus subtilis
37. Complete nucleotide sequence of a gene coding for heat and pH stable α-amylase of Bacillus licheniformis: Comparison of the amino acid sequence of three bacterial liquifying α-amylase deduced from the DNA sequences