Immunohistochemical identification of multipotent adult progenitor cells from human bone marrow after transplantation into the rat brain

Brain Research Protocols

Volumn 11, Issue 1, 2003, Pages 38-45

  Zhao, Li Ru ^a   Duan, Wei Ming ^a   Reyes, Morayma ^a,b   Verfaillie, Catherine M ^a,b   Low, Walter C ^a,b  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Bone marrow stem cell; Ethanol; Fast frozen; Formaldehyde; Transplantation

Indexed keywords

ANIMAL MODEL; ANIMAL TISSUE; ANTIGENICITY; ARTICLE; BRAIN ISCHEMIA; BRAIN TISSUE; CELL LABELING; CONTROLLED STUDY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HOST CELL; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMMUNOPEROXIDASE STAINING; IMMUNOREACTIVITY; NONHUMAN; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; STEM CELL; TISSUE FIXATION; TISSUE PREPARATION;

EID: 0037356193     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(03)00014-X     Document Type: Article

References (38)

1. Baker-Cairns B., Meyers K., Hamilton R., Smith C., Tornatore C. Immunohistochemical staining of fixed tissue using antigen retrieval and a thermal cycler. Biotechniques. 20:1996;641-650.
2. Battifora H. Assessment of antigen damage in immunohistochemistry. The vimentin internal control. Am. J. Clin. Pathol. 96:1991;669-671.
3. Beresford J.N., Bennett J.H., Devlin C., Leboy P.S., Owen M.E. Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J. Cell Sci. 102:1992;341-351.
4. Boon M.E., Kok L.P. Microwaves for immunohistochemistry. Micron. 25:1994;151-170.
5. Bowers B., Maser M. Artifacts in fixation for transmission electron microscopy. Crang R.F.E., Klomparens K.L. Artifacts in Biological Electron Microscopy. 1988;13-42 Plenum, New York.
6. Brazelton T.R., Rossi F.M., Keshet G.I., Blau H.M. From marrow to brain: expression of neuronal phenotypes in adult mice. Science. 290:2000;1775-1779.
7. Coyle P. Middle cerebral artery occlusion in the young rat. Stroke. 13:1982;855-859.
8. Curran R.C., Gregory J. Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections. J. Clin. Pathol. 31:1978;974-983.
9. Dennis J.E., Haynesworth S.E., Young R.G., Caplan A.I. Osteogenesis in marrow-derived mesenchymal cell porous ceramic composites transplanted subcutaneously: effect of fibronectin and laminin on cell retention and rate of osteogenic expression. Cell Transplant. 1:1992;23-32.
10. Eglitis M.A., Mezey E. Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice. Proc. Natl. Acad. Sci. USA. 94:1997;4080-4085.
11. Farmilo A.J., Stead R.H. Fixation in immunocytochemistry. Naish S.J. Immunochemical Staining Methods. 1989;24-29 DAKO, Carpinteria.
12. Fox C.H., Johnson F.B., Whiting J., Roller P.P. Formaldehyde fixation. J. Histochem. Cytochem. 33:1985;845-853.
13. Horrobin D.F. Lipid metabolism, human evolution and schizophrenia. Prostaglandins Leukot. Essent. Fatty Acids. 60:1999;431-437.
14. Huang S.N., Minassian H., More J.D. Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab. Invest. 35:1976;383-390.
15. Jassim A. AJ-FS9 monoclonal antibody detects masked antigens with the human sperm tail fibrous sheath. Hum. Reprod. 9:1994;1836-1844.
16. Jiang Y., Jahagirdar B.N., Reinhardt R.L., Schwartz R.E., Keene C.D., Ortiz-Gonzalez X.R., Reyes M., Lenvik T., Lund T., Blackstad M., Du J., Aldrich S., Lisberg A., Low W.C., Largaespada D.A., Verfaillie C.M. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 418:2002;41-49.
17. Kingsbury A.E., Bray E.L., Foster O.J. A simplified and rapid procedure for in situ hybridization on human, flash-frozen, post-mortem brain and its combination with immunohistochemistry. J. Neurosci. Methods. 69:1996;213-227.
18. Kokaia Z., Metsis M., Kokaia M., Bengzon J., Elmer E., Smith M.L., Timmusk T., Siesjo B.K., Persson H., Lindvall O. Brain insults in rats induce increased expression of the BDNF gene through differential use of multiple promoters. Eur. J. Neurosci. 6:1994;587-596.
19. Kopen G.C., Prockop D.J., Phinney D.G. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc. Natl. Acad. Sci. USA. 96:1999;10711-10716.
20. Melan M.A. Overview of cell fixation and permeabilization. Methods Mol. Biol. 34:1994;55-66.
21. Mezey E., Chandross K.J., Harta G., Maki R.A., McKercher S.R. Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science. 290:2000;1779-1782.
22. Morris R.J., Barber P.C. Fixation of Thy-1 in nervous tissue for immunohistochemistry: a quantitative assessment of the effect of different fixation conditions upon retention of antigenicity and the cross-linking of Thy-1. J. Histochem. Cytochem. 31:1983;263-274.
23. Myers J.D. Development and application of immunocytochemical staining techniques: a review. Diagn. Cytopathol. 5:1989;318-330.
24. Nicolas G. Advantages of fast-freeze fixation followed by freeze-substitution for the preservation of cell integrity. J. Electron Microsc. Tech. 18:1991;395-405.
25. Pereira R.F., Halford K.W., O'Hara M.D., Leeper D.B., Sokolov B.P., Pollard M.D., Bagasra O., Prockop D.J. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice. Proc. Natl. Acad. Sci. USA. 92:1995;4857-4861.
26. Pereira R.F., O'Hara M.D., Laptev A.V., Halford K.W., Pollard M.D., Class R., Simon D., Livezey K., Prockop D.J. Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta. Proc. Natl. Acad. Sci. USA. 95:1998;1142-1147.
27. Petersen B.E., Bowen W.C., Patrene K.D., Mars W.M., Sullivan A.K., Murase N., Boggs S.S., Greenberger J.S., Goff J.P. Bone marrow as a potential source of hepatic oval cells. Science. 284:1999;1168-1170.
28. Pow D.V., Wright L.L., Vaney D.I. The immunocytochemical detection of amino-acid neurotransmitters in paraformaldehyde-fixed tissues. J. Neurosci. Methods. 56:1995;115-123.
29. Riederer B.M., Porchet R., Marugg R.A., Binder L.I. Solubility of cytoskeletal proteins in immunohistochemistry and the influence of fixation. J. Histochem. Cytochem. 41:1993;609-616.
30. Rustad O.J., Kaye V., Cerio R., Zachary C.B. Postfixation of cryostat sections improves tumor definition in Mohs surgery. J. Dermatol. Surg. Oncol. 15:1989;1262-1267.
31. Sanchez-Ramos J., Song S., Cardozo-Pelaez F., Hazzi C., Stedeford T., Willing A., Freeman T.B., Saporta S., Janssen W., Patel N., Cooper D.R., Sanberg P.R. Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp. Neurol. 164:2000;247-256.
32. Scanziani E. Immunohistochemical staining of fixed tissues. Methods Mol. Biol. 104:1998;133-140.
33. Shi S.R., Key M.E., Kalra K.L. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39:1991;741-748.
34. Wakitani S., Saito T., Caplan A.I. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve. 18:1995;1417-1426.
35. Werner M., Von Wasielewski R., Komminoth P. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem. Cell. Biol. 105:1996;253-260.
36. Woodbury D., Schwarz E.J., Prockop D.J., Black I.B. Adult rat and human bone marrow stromal cells differentiate into neurons. J. Neurosci. Res. 61:2000;364-370.
37. Zhao L.R., Duan W.M., Reyes M., Keene C.D., Verfaillie C.M., Low W.C. Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats. Exp. Neurol. 174:2002;11-20.
38. Zhao L.R., Mattsson B., Johansson B.B. Environmental influence on brain-derived neurotrophic factor messenger RNA expression after middle cerebral artery occlusion in spontaneously hypertensive rats. Neuroscience. 97:2000;177-184.