Use of the formazan dye zincon for staining proteins in polyacrylamide gels

BioTechniques

Volumn 34, Issue 3, 2003, Pages 486-488

  Smejkal, Gary B ^a,b   Hoff, Henry F ^b  

^a Proteome Systems   (United States)

^b LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANAZOLENE SODIUM; BENZENE DERIVATIVE; CHELATING AGENT; DYE; FORMAZAN; POLYACRYLAMIDE GEL; PROTEIN; UNCLASSIFIED DRUG; ZINCON;

ACCURACY; ARTICLE; COLLOID; DISPERSION; INTERMETHOD COMPARISON; PROTEIN ANALYSIS; PROTEIN BINDING; STAINING;

EID: 0037337888     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03343bm07     Document Type: Article

References (15)

1. Corns, K.M. 1987. A new colorimetric method for the measurement of serum calcium using a zinc-zincon indicator. Ann. Clin. Biochem. 24:591-597.
2. Hasan, M. 1977. A method for demonstrating zinc content of the brain using 2-carboxy-2′-hydroxy-5′-sulfoformazybenzene perfusion staining. Experimentia 33:552-553.
3. Monreal, J.I., C. Viader, C. Perez-Barquero, N. Lopez-Moratalla, P. DePablo, and E. Santiago. 1987. Insulin-receptor interactions. Possible involvement of metals. Rev. Esp. Fisiol. 43:133-140.
4. Mollinedo, F., N. Lopez-Moratalla, J.P. Pivel, V. Larraga, E. Santiago, and E. Munoz. 1981. Identification of a bacterial energy-transducing ATPase as a metalloprotein. Effect of chelating agents and divalent metal ions on ATPase activity. Eur. J. Biochem. 119:183-188.
5. Ono, H. and A. Ito. 1984. Transport of the precursor for sulfite oxidase into intermembrane space of liver mitochondria: characterization of import and processing activities. J. Biochem. 95:345-352.
6. 2+ chelates with zincon. Egypt. J. Chem. 28:367-374.
7. Miller, D.C. 1979. Colorimetric determination of zinc with zincon and cyclohexanone, J. Water Poll. Control Fed. 51:2402-2412.
8. Laemmli, U.K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680-685.
9. Shainoff, J.R., G.B. Smejkal, O. Mitkevich, and P.M. DiBello. 1996. Preparative electrophoresis on linear polyacrylamide-agarose composite gels. Electrophoresis 17:179-184.
10. Ehrenwald, E. and P.L. Fox. 1994. Isolation of nonlabile human ceruloplasmin by chromatographic removal of a plasma metalloproteinase. Arch. Biochem. Biophys. 309:392-395.
11. Emerson, M.F. and A. Holtzer. 1967. The hydrophobic bond in micellar systems: effects of various additives on the stability of micelles of sodium dodecylsulfate and of dodecyltrimethylammonium bromide. J. Phys. Chem. 71:3320-3330.
12. Righetti, P.G. and F. Chillemi. 1978. Isoelectric focusing of peptides. J. Chromatogr. 157:243-251.
13. Alba, F.J., A. Bermudez, S. Bartolome, and J.R. Daban. 1996. Detection of five nanograms of protein by two-minute Nile Red staining of unfixed SDS gels. BioTechniques 21:625-626.
14. Steinburg, T.H., R.P. Haugland, and V.L. Singer. 1996. Applications of SYPRO Orange and SYPRO Red protein gel stains. Anal. Biochem. 239:238-245.
15. Hilario, E., I. Romero, and H. Celis. 1990. Determination of the physicochemical constants and spectrophotometric characteristic of the metallochrome zincon and its potential use in biological systems. J. Biochem. Biophys. Methods 21:197-207.