Detection of five nanograms of protein by two-minute Nile red staining of unfixed SDS gels

BioTechniques

Volumn 21, Issue 4, 1996, Pages 625-626

  Alba, F Javier ^a   Bermudez, Antonio ^a   Bartolome, Salvador ^a   Daban, Joan Ramon ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; PROTEIN;

ACCURACY; ARTICLE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN ELECTROPHORESIS;

EID: 0029785457     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214bm12     Document Type: Article

References (10)

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2. Daban, J.-R., S. Bartolome and A. Bermudez. 1996. Rapid Staining of proteins in polyacrylamide gels with Nile red, p. 179-185. In J.M. Walker (Ed.), The Protein Protocols Handbook. Humana Press, Totowa, NJ.
3. Daban, J.-R., S. Bartolome and M. Samso. 1991. Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 199:169-174.
4. Daban, J.-R., M. Samso and S. Bartolome. 1991. Use of Nile red as a fluorescent probe for the study of the hydrophobic properties of protein-sodium dodecyl sulfate complexes in solution. Anal. Biochem. 199:162-168.
5. Garfin, D.E. 1990. One-dimensional gel electrophoresis, p. 425-441. In M.P. Deuscher (Ed.), Methods in Enzymology, Vol. 182. Academic Press, New York.
6. Greenspan, P., E.P. Mayer and S.D. Fowler. 1985. Nile red: A selective fluorescent stain for intracellular lipid droplets. J. Cell. Biol. 100:965-973.
7. Hames, B.D. 1990. One-dimensional polyacrylamide gel electrophoresis, p. 1-147. In B.D. Hames and D. Rickwood (Eds.), Gel Electrophoresis of Proteins. A Practical Approach. IRL Press, Oxford.
8. Samso, M., J.-R. Daban, S. Hansen and G.R. Jones. 1995. Evidence for sodium dodecyl sulfate/protein complexes adopting a necklace structure. Eur. J. Biochem. 232:818-824.
9. Wirth, P.J. and A. Romano. 1995. Staining methods in gel electrophoresis, including the use of multiple detection methods. J. Chromatogr. A 698:123-143.
10. Zewert, T.E. and M.G. Harrington. 1993. Protein electrophoresis. Curr. Opin. Biotechnol. 4:3-8.