Regulation of L-cystine transport and intracellular GSH level by a nitric oxide donor in primary cultured rabbit conjunctival epithelial cell layers

Investigative Ophthalmology and Visual Science

Volumn 44, Issue 3, 2003, Pages 1202-1210

  Gukasyan, Hovhannes J ^a   Kannan, Ram ^a   Lee, Vincent H L ^a   Kim, Kwang Jin ^a,b  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTINE; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; N ACETYL S NITROSOPENICILLAMINE; NITRIC OXIDE; NITRIC OXIDE DONOR; SODIUM ION; SULFUR AMINO ACID;

AMINO ACID METABOLISM; AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; CELL LYSATE; CONCENTRATION RESPONSE; CONJUNCTIVA EPITHELIUM; CONTROLLED STUDY; ENZYME MECHANISM; ENZYME SUBUNIT; HOMEOSTASIS; MALE; MEMBRANE PERMEABILITY; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABBIT; REGULATORY MECHANISM; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELLS, CULTURED; CONJUNCTIVA; CYSTINE; EPITHELIAL CELLS; GLUTAMATE-CYSTEINE LIGASE; GLUTATHIONE; MALE; NITRIC OXIDE DONORS; PENICILLAMINE; PERMEABILITY; RABBITS; SODIUM;

EID: 0037336963     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: 10.1167/iovs.02-0409     Document Type: Article

References (40)

1. Kaplowitz N, Aw TY, Ookhtens M. The regulation of hepatic glutathione. Annu Rev Pharmacol Toxicol. 1985;25:715-744.
2. Meister A, Anderson ME. Glutathione. Annu Rev Biochem. 1983; 52:711-760.
3. Nucci C, Palamara AT, Ciriolo MR, et al. Imbalance in corneal redox state during herpes simplex virus 1-induced keratitis in rabbits: effectiveness of exogenous glutathione supply. Exp Eye Res. 2000;70:215-220.
4. Palamara AT, Perno CF, Ciriolo MR, et al. Evidence for antiviral activity of glutathione: in vitro inhibition of herpes simplex virus type 1 replication. Antiviral Res. 1995:27:237-253.
5. Gukasyan HJ, Lee VHL, Kim KJ, Kannan R. Net glutathione secretion across primary cultured rabbit conjunctival epithelial cell layers. Invest Opbthalmol Vis Sci. 2002;43:1154-1161.
6. Bannai S, Ishii T. Transport of cystine and cysteine and cell growth in cultured human diploid fibroblasts: effect of glutamate and homocysteate. J Cell Physiol. 1982;112:265-272.
7. Makowske M, Christensen HN. Hepatic transport system interconverted by protonation from service for neutral to service for anionic amino acids. J Biol Chem. 1982;257:14635-14638.
8. Makowske M, Christensen HN. Contrasts in transport systems for anionic amino acids in hepatocytes and a hepatoma cell line HTC. J Biol Chem. 1982;257:5663-5670.
9. Watanabe H, Bannai S. Induction of cystine transport activity in mouse peritoneal macrophages. J Exp Med. 1987;165:628-640.
10. Knickelbein RG, Seres T, Lam G, Johnston RB Jr, Warshaw JB. Characterization of multiple cysteine and cystine transporters in rat alveolar type II cells. Am J Physiol. 1997;273:L1147-L1155.
11. Bukowski DM, Deneke SM, Lawrence RA, Jenkinson SG. A noninducible cystine transport system in rat alveolar type II cells. Am J Physiol. 1995;268:L21-L26.
12. Bridges CC, Kekuda R, Wang H, et al. Structure, function, and regulation of human cystine/glutamate transporter in retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2001;42:47-54.
13. Bannai S. Transport of cystine and cysteine in mammalian cells. Biochim Biophys Acta. 1984;779:289-306.
14. Smith QR, Stoll J. Blood brain barrier amino acid transport. In: Pardridge WM, ed. Introduction to the Blood-Brain Barrier. Cambridge, UK: Cambridge University Press; 1998; 188-197.
15. Sato H, Tamba M, Ishii T, Bannai S. Cloning and expression of a plasma membrane cystine/glutamate exchange transporter composed of two distinct proteins. J Biol Chem. 1999;274:11455-11458.
16. Palacin M, Estevez R, Bertran J, Zorzano A. Molecular biology of mammalian plasma membrane amino acid transporters. Physiol Rev. 1998;78:969-1054.
17. Miura K, Ishii T, Sugita Y, Bannai S. Cystine uptake and glutathione level in endothelial cells exposed to oxidative stress. Am J Physiol. 1992;262:C50-C58.
18. Ochi T. Arsenic compound-induced increases in glutathione levels in cultured Chinese hamster V79 cells and mechanisms associated with changes in gamma-glutamylcysteine synthetase activity, cystine uptake and utilization of cysteine. Arch Toxicol. 1997;71:730-740.
19. Tomi M, Hosoya KI, Takanaga H, Ohtsuki S, Terasaki T. Induction of xCT gene expression and L-cystine transport activity by diethyl maleate at the inner blood-retinal barrier. Invest Ophthalmol Vis Sci. 2002;43:774-779.
20. Saha P, Kim KJ, Lee VHL. A primary culture model of rabbit conjunctival epithelial cells exhibiting tight barrier properties. Curr Eye Res. 1996;15:1163-1169.
21. Yang JJ, Ueda H, Kim KJ, Lee VHL. Meeting future challenges in topical ocular drug delivery: development of an air-interfaced primary culture of rabbit conjunctival epithelial cells on a permeable support for drug transport studies. J Control Release. 2000; 65:1-11.
22. Kompella UB, Kim KJ, Lee VHL. Active chloride transport in the pigmented rabbit conjunctiva. Curr Eye Res. 1993;12:1041-1048.
23. +-dependent GSH transport in HCEC. Brain Res. 2000;852:374-382.
24. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969;27:502-522.
25. Fariss MW, Reed DJ. High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives. Methods Enzymol. 1987;143:101-109.
26. Lu SC, Bao Y, Huang ZZ, Sarthy VP, Kannan R. Regulation of gamma-glutamylcysteine synthetase subunit gene expression in retinal Muller cells by oxidative stress. Invest Ophthalmol Vis Sci. 1999;40:1776-1782.
27. Kannan R, Ouyang B, Wawrousek E, Kaplowitz N, Andley UP. Regulation of GSH in alphaA-expressing human lens epithelial cell lines and in alphaA knockout mouse lenses. Invest Ophthalmol Vis Sci. 2001;42:409-416.
28. Olney JW, Zorumski C, Price MT, Labruyere J. L-cysteine, a bicarbonate-sensitive endogenous excitotoxin. Science. 1990;248:596-599.
29. Knickelbein RG, Seres T, Lam G, Johnston RB Jr, Warshaw JB. Characterization of multiple cysteine and cystine transporters in rat alveolar type II cells. Am J Physiol. 1997;273:LII47-LII55.
30. Hosoya KI, Tomi M, Ohtsuki S, et al. Enhancement of L-cystine transport activity and its relation to xCT gene induction at the blood-brain barrier by diethyl maleate treatment. J Pharmacol Exp Ther. 2002;302:225-231.
31. Desjeux JF, Vonlanthen M, Dumontier AM, Simell O, Legrain M. Cystine fluxes across the isolated jejunal epithelium in cystinuria: increased efflux permeability at the luminal membrane. Pediatr Res. 1987;21:477-481.
32. Schafer JA, Watkins ML. Transport of L-cystine in isolated perfused proximal straight tubules. Pflugers Arch. 1984;401:143-151.
33. Choy CK, Cho P, Chung WY, Benzie IF. Water-soluble antioxidants in human tears: effect of the collection method. Invest Ophthalmol Vis Sci. 2001;42:3130-3134.
34. Lieberman MW, Wiseman AL, Shi ZZ, et al. Growth retardation, cysteine deficiency in gamma-glutamyl transpeptidase-deficient mice. Proc Natl Acad Sci USA. 1996;93:7923-7926.
35. Karp DR, Shimooku K, Lipsky PE. Expression of gamma-glutamyl transpeptidase protects Ramos B cells from oxidation-induced cell death. J Biol Chem. 2001;276:3798-3804.
36. Kim KJ, Gukasyan HJ, Kannan R, Lee VHL. Sulfur amino acid transport in conjunctiva: asymmetry of cystine transport in primary cultured rabbit conjunctival epithelial cells (RCEC). Invest Ophthalmol Vis Sci. 2001;42(4):S501. Abstract nr 2706.
37. +-dependent L-arginine transport in the pigmented rabbit conjunctiva. Exp Eye Res. 1997; 65:547-553.
38. Kowaluk EA, Fung HL. Spontaneous liberation of nitric oxide cannot account for in vitro vascular relaxation by S-nitrosothiols. J Pharmacol Exp Ther. 1990;255:1256-1264.
39. Feelisch M. The biochemical pathways of nitric oxide formation from nitrovasodilators: appropriate choice of exogenous NO donors and aspects of preparation and handling of aqueous NO solutions. J Cardiovasc Pharmacol. 1991; 17(suppl 3):S25-S33.
40. Lander HM, Sehajpal P, Levine DM, Novogrodsky A. Activation of human peripheral blood mononuclear cells by nitric oxide-generating compounds. J Immunol. 1993;150:1509-1516.