Preservation of fluorescent protein activity in tumor tissue

BioTechniques

Volumn 34, Issue 3, 2003, Pages 476-480

  Rosenberg, E ^b   Holmes, M ^b   Tenenholz, T ^b   Abd El Raouf Khalil, A ^b   Valerie, K ^a,b  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN; RNA;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ARTICLE; DNA MICROARRAY; FEMALE; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN FUNCTION; RADIATION RESPONSE; TISSUE PRESERVATION; TUMOR CELL; TUMOR GROWTH;

ANIMALIA;

EID: 0037336379     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03343bm05     Document Type: Article

References (11)

1. Hoffman, R.M. 2001. Visualization of GFP-expressing tumors and metastasis in vivo. BioTechniques 30:1016-1024.
2. Naumov, G.N., S.M. Wilson, I.C. MacDonald, E.E. Schmidt, V.L. Morris, A.C. Groom, R.M. Hoffman, and A.F. Chambers. 1999. Cellular expression of green fluorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis. J. Cell Sci. 112:1835-1842.
3. Wang, S., H. Wu, J. Jiang, T.M. Delohery, F. Isdell, and S.A. Goldman. 1998. Isolation of neuronal precursors by sorting embryonic forebrain transfected with GFP regulated by the T alpha 1 tubulin promoter. Nat. Biotechnol. 16:196-201.
4. Yang, M., E. Baranov, P. Jiang, F.X. Sun, X.M. Li, L. Li, S. Hasegawa, M. Bouvet, et al. 2000. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97:1206-1211.
5. Barrett, M.T., J. Glogovac, L.J. Prevo, B.J. Reid, P. Porter, and P.S. Rabinovitch. 2002. High-quality RNA and DNA from flow cytometrically sorted human epithelial cells and tissues. BioTechniques 32:888-896.
6. Florell, S.R., C.M. Coffin, J.A. Holden, J.W. Zimmermann, J.W. Gerwels, B.K. Summers, D.A. Jones, and S.A. Leachman. 2001. Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod. Pathol. 14:116-128.
7. Valerie, K., W. Hawkins, J. Farnsworth, R.K. Schmidt-Ullrich, P.-S. Lin, C. Amir, and J. Feden. 2001. Substantially improved in vivo radiosensitization of rat glioma with mutant HSV-TK and acyclovir. Cancer Gene Ther. 8:3-8.
8. Moritz, O.L., B.M. Tam, B.E. Knox, and D.S. Papermaster. 1999. Fluorescent photoreceptors of transgenic Xenopus laevis imaged in vivo by two microscopy techniques. Invest. Ophthalmol. Vis. Sci. 40:3276-3280.
9. Walter, I., M. Fleischmann, D. Klein, M. Muller, B. Salmons, W.H. Gunzburg, M. Renner, and W. Gelbman. 2000. Rapid and sensitive detection of enhanced green fluorescent protein expression in paraffin sections by confocal laser scanning microscopy. Histochem. J. 32:99-103.
10. Brust, D., J. Feden, J. Farnsworth, C. Amir, W.C. Broaddus, and K. Valerie. 2000. Radiosensitization of rat glioma with bromodeoxycytidine and adenovirus expressing HSV-TK delivered by slow positive pressure infusion. Cancer Gene Ther. 7:879-884.
11. Lal, S., M. Lacroix, P. Tofilon, G.N. Fuller, R. Sawaya, and F.F. Lang. 2000. An implantable guide-screw system for brain tumor studies in small animals. J. Neurosurg. 92:326-333.