Entirely automated quantification of human immunodeficiency virus type 1 (HIV-1) RNA in plasma by using the ultrasensitive COBAS AMPLICOR HIV-1 Monitor test and RNA purification on the MagNA pure LC instrument

Journal of Clinical Microbiology

Volumn 41, Issue 3, 2003, Pages 1248-1251

  Hölzl, Gabriele ^a   Stöcher, Markus ^a   Leb, Victoria ^a   Stekel, Herbert ^a   Berg, Jörg ^a  

^a KEPLER UNIVERSITY HOSPITAL   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ARTICLE; DEVICE; EVALUATION; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; LABORATORY AUTOMATION; LABORATORY TEST; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE GENETICS; RELIABILITY; REPRODUCIBILITY; RNA ANALYSIS; RNA PURIFICATION; TECHNIQUE; VIRUS DETECTION; VIRUS LOAD;

AUTOMATION; HIV-1; HUMANS; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0037335871     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.3.1248-1251.2003     Document Type: Article

References (17)

1. DiDomenico, N., H. Link, R. Knobel, T. Caratsch, W. Weschler, Z. G. Loewy, and M. Rosenstraus. 1996. COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR. Clin. Chem. 42:1915-1923.
2. Erali, M., and D. Hillyard. 1999. Evaluation of the ultrasensitive Roche Amplicor HIV-1 Monitor assay for the quantification of human immunodeficiency virus type 1 RNA. J. Clin. Microbiol. 37:792-795.
3. Espy, M. J., P. N. Rys, A. D. Wold, J. R. Uhl, L. M. Sloan, G. D. Jenkins, D. M. Ilstrup, F. R. Cockerill III, R. Patel, J. E. Rosenblatt, and T. F. Smith. 2001. Detection of herpes simplex virus DNA in genital and dermal specimens by LightCycler PCR after extraction using the IsoQuick, MagNA Pure, and BioRobot 9604 methods. J. Clin. Microbiol. 39:2233-2236.
4. Fischer, M., W. Huber, A. Kallivroussis, P. Ott, M. Opravil, R. Lüthy, R. Weber, and R. W. Cone. 1999. Highly sensitive methods for quantitation of human immunodeficiency virus type 1 RNA from plasma, cells, and tissues. J. Clin. Microbiol. 37:1260-1264.
5. Gulick, R. M., J. W. Mellors, D. Havlir, J. J. Eron, C. Gonzalez, D. McMahon, D. D. Richman, F. T. Valentine, L. Jonas, A. Meibohm, E. A. Emini, and J. A. Chodakewitz. 1997. Treatment with indinavir, zidovudine, and lamivudine in adults with human immunodeficiency virus infection and prior antiretroviral therapy. N. Engl. J. Med. 337:734-739.
6. Holguin, A., B. Aracil, A. Alvarez, C. Barros, and V. Soriano. 2001. Prevalence of human immunodeficiency virus type 1 (HIV-1) non-B subtypes in foreigners living in Madrid, Spain, and comparison of the performances of the AMPLICOR HIV-1 Monitor Version 1.0 and the new automated Version 1.5. J. Clin. Microbiol. 39:1850-1854.
7. Jungkind, D. 2001. Automation of laboratory testing for the infectious diseases using the polymerase chain reaction - our past, our present, our future. J. Clin. Virol. 20:1-6.
8. Kessler, H. H., G. Mühlbauer, E. Stelzl, E. Daghofer, B. I. Santner, and E. Marth. 2001. Fully automated nucleic acid extraction: MagNA Pure LC. Clin. Chem. 47:1124-1126.
9. Li, T. S., R. Tubianan, C. Katlama, V. Calvez, H. Ait Mohand, and B. Autran. 1998. Long-lasting recovery in CD4 T-cell function and viral-load reduction after highly active antiretroviral therapy in advanced HIV-1 disease. Lancet 351:1682-1686.
10. Loeffler, J., N. Henke, H. Hebart, D. Schmidt, L. Hagmeyer, U. Schumacher, and H. Einsele. 2000. Quantification of fungal DNA by using fluorescence resonance energy transfer and the LightCycler system. J. Clin. Microbiol. 38:586-590.
11. Muir, D., D. White, J. King, N. Verlander, and D. Pillary. 2000. Predictive value of the ultrasensitive HIV viral load assay in clinical practice. J. Med. Virol. 61:411-416.
12. Murphy, E. L., A. C. Collier, L. A. Kalish, S. F. Assmann, M. F. Para, T. P. Flanigan, P. N. Kumar, L. Mintz, F. R. Wallach, and G. J. Nemo. 2001. Highly active antiretroviral therapy decreases mortality and morbidity in patients with advanced HIV disease. Ann. Intern. Med. 135:17-26.
13. Notermans, D. W., S. Jurriaans, F. de Wolf, N. A. Foudraine, J. J. de Jong, W. Cavert, C. M. Schuwirth, R. H. Kauffmann, P. L. Meenhorst, H. McDade, C. Goodwin, J. M. Leonard, J. Goudsmit, S. A. Danner, et al. 1998. Decrease of HIV-1 RNA levels in lymphoid tissue and peripheral blood during treatment with ritonavir, lamivudine and zidovudine. AIDS 12:167-173.
14. Revets, H., D. Marissens, S. de Wit, P. Lacor, N. Clumeck, S. Lauwers, and G. Zissis. 1996. Comparative evaluation of NASBA HIV-1 RNA QT, AMPLICOR-HIV Monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma. J. Clin. Microbiol. 34:1058-1064.
15. Saag, M. S., M. Holodiny, D. R. Kuritzkes, W. A. O'Brien, R. Coombs, M. E. Poscher, D. M. Jacobsen, G. M. Shaw, D. D. Richman, and P. A. Volberding. 1996. HIV viral load markers in clinical practice. Nat. Med. 2:625-629.
16. Sun, R., J. Ku, H. Jayakar, J. C. Kuo, D. Brambilla, S. Herman, M. Rosenstraus, and J. Spadoro. 1998. Ultrasensitive reverse transcription-PCR assay for quantification of human immunodeficiency virus type 1 RNA in plasma. J. Clin. Microbiol. 36:2964-2969.
17. Yeni, P. G., S. M. Hammer, C. C. Carpenter, C. A. Cooper, M. A. Fischl, J. M. Gatell, and B. G. Gazzard. 2002. Antiretroviral treatment for adult HIV infection in 2002: updated recommendations of the International AIDS Society-USA Panel. JAMA 288:222-235.