In vitro metabolism of the glycosidic sweeteners, stevia mixture and enzymatically modified stevia in human intestinal microflora

Food and Chemical Toxicology

Volumn 41, Issue 3, 2003, Pages 359-374

  Koyama, E ^a   Kitazawa, K ^a   Ohori, Y ^a   Izawa, O ^a   Kakegawa, K ^b   Fujino, A ^a   Ui, M ^c  

^a MITSUBISHI CHEMICAL CORPORATION   (Japan)

^b Maruzen Pharmaceuticals Co   (Japan)

^c TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

Author keywords

Enzymatically modified stevia; Human intestinal microflora; LC ESI MS; Stevia mixture; Steviol

Indexed keywords

AGLYCONE; ALPHA MONOGLUCOSYLREBAUDIOSIDE A; ALPHA MONOGLUCOSYLSTEVIOSIDE; GLYCOSIDE; REBAUDIOSIDE A; STEVIA MIXTURE; STEVIOL; STEVIOSIDE; SWEETENING AGENT; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CARBOHYDRATE METABOLISM; CHEMICAL MODIFICATION; CONTROLLED STUDY; DEGRADATION; FECES; FOOD ANALYSIS; HOMOGENATE; HUMAN; HYDROLYSIS; IN VITRO STUDY; INCUBATION TIME; INTESTINE FLORA; MALE; NORMAL HUMAN; STEVIA;

ADULT; ANAEROBIOSIS; ANIMALS; BACTERIA, ANAEROBIC; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, LIQUID; DIGESTIVE SYSTEM; DITERPENES; DITERPENES, KAURANE; FECES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUCOSIDES; HUMANS; HYDROLYSIS; KINETICS; MALE; MOLECULAR WEIGHT; PLANT EXTRACTS; RATS; SPECIES SPECIFICITY; STEVIA; STRUCTURE-ACTIVITY RELATIONSHIP; SWEETENING AGENTS; TERPENES;

STEVIA REBAUDIANA;

EID: 0037333120     PISSN: 02786915     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0278-6915(02)00235-1     Document Type: Article

References (20)

1. Aze Y., Toyoda K., Imaida K., Hayashi S., Imazawa T., Hayashi Y., Takahashi M. Subchronic oral toxicity study of stevioside in F344 rats. Bulletin of the National Institute Hygienic Sciences. 1991;48-54.
2. 131I-stevioside and its metabolites. Nuclear and Medical Biology. 23:1996;97-100.
3. Compadre C.M., Hussain R.A., Nanayakkara N.P.D., Pezzuto J.M., Kinghorn A.D. Mass spectral analysis of some derivatives and in vitro metabolites of steviol, the aglycone of the natural sweeteners, stevioside, rebaudioside A, and rubusoside. Biomedical and Environmental Mass Spectrometry. 15:1988;211-222.
4. Finegold S.M., Attebery H.R., Sutter V.L. Effect of diet on human faecal flora: comparison of Japanese and American diets. American Journal of Clinical Nutrition. 27:1974;1456-1469.
5. Hanson J.R., De Oliveira B.H. Stevioside and related sweet diterpenoid glycosides. National Products Report. 10:1993;301-309.
6. Hutapea A.M., Toskulkao C., Buddhasukh D., Wilairat P., Glinsukon T. Digestion of stevioside, a natural sweetener, by various digestive enzymes. Journal of Clinical Biochemistry and Nutrition. 23:1997;177-186.
7. Lucky T.D. Introduction of intestinal microecology. American Journal of Clinical Nutrition. 25:1972;1292-1294.
8. Matsui M., Matsui K., Kawasaki Y., Oda T., Noguchi T., Kitagawa Y., Sawada M., Hayashi M., Nohmi T., Yoshihira K., Ishidate M., Sofuni T. Evaluation of the genotoxicity of stevioside and steviol using six in vitro and one in vivo mutagenicity assays. Mutagenesis. 11:1996;573-579.
9. Matsui M., Sofuni T., Nohmi T. Regionally-targeted mutagenesis by metabolically-activated steviol: DNA sequence analysis of steviol-induced mutants of guanine phophoribosyltransferase (gpt) gene of Salmonella typhimurium TM677. Mutagenesis. 11:1996;565-572.
10. Mitsuoka T. Intestinal Microbiology, first ed. 1990;Asakura Shoten, Tokyo. Growth, movement and distribution in intestinal flora.
11. Mori N., Sakanoue M., Takeuchi M., Shimpo K., Tanabe T. Effect of stevioside on fertility in rats. Journal of the Food and Hygienic Society, Japan. 22:1981;409-414.
12. Nakayama K., Kasahara D., Yamamoto F. Absorption, distribution, metabolism and excretion of stevioside in rats. Journal of the Food and Hygienic Society, Japan. 27:1986;1-8.
13. Pezzuto J.M., Compadre C.M., Swanson S.M., Nanayakkara N.P.D., Kinghorn A.D. Metabolically activated steviol, the aglycone of stevioside, is mutagenic. Proceedings of the National Academy of Sciences of the USA. 82:1985;2478-2482.
14. Sennma M., Fujii M., Okada Y., Kataoka H., Ito Y. Development of rapid analysis of stevia sweeteners and an approach to study metabolism of component of stevia by intestinal microbes. Japanese Journal of Food Chemistry. 8:2001;105-111.
15. Soejarto D.D., Kinghorn A.D., Farnsworth N.R. Potential sweetening agents of plant origin. III. Organoleptic evaluation of Stevia leaf herbarium samples for sweetness. Journal of Natural Products. 45:1982;590-599.
16. Toskulkao C., Chaturat L., Temcharoen P., Glinsukon T. Acute toxicity of stevioside, a natural sweetener, and its metabolite, steviol, in several animal species. Drug and Chemical Toxicology. 20:1997;31-44.
17. Toyoda, K., Kawanishi, T., Ueyama, C., Takahashi, M. 1995. Re-Evaluation of the Safety of a Food Additive (Reported in Fiscal 1994). A Chronic Toxicity/Carcinogenicity Study of Stevioside (A Substance Extracted from Stevia): Final Report. Unpublished report from Division of Pathology, Biological Safety Research Center, National Institute of Health Sciences, Japan. Submitted to WHO by Ministry of Health and Welfare, Food Chemistry Division, Japan.
18. Toyoda K., Matsui H., Shoda T., Ueyama C., Takeda K., Takahashi M. Assessment of the carcinogenicity of stevioside in F344 rats. Food and Chemical Toxicology. 35:1997;597-603.
19. Wingard R.E. jr., Brown J.P., Enderlin F.E., Dale J.A., Seitz C.T. Intestinal degradation and absorption of the glycosidic sweeteners stevioside and rebaudioside A. Experientia. 36:1980;519-520.
20. Xili L., Chengjiany B., Eryi X., Reiming S., Yuengming W., Haodong S., Zhiyian H. Chronic oral toxicity and carcinogenicity study of stevioside in rats. Food and Chemical Toxicology. 30:1992;957-965.