Modification of Job's method for determining the stoichiometry of protein-protein complexes

Analytical Biochemistry

Volumn 313, Issue 1, 2003, Pages 170-172

  Facchiano, Antonio ^a   Ragone, Raffaele ^b  

^a LABORATORIO DI PATOLOGIA VASCOLARE   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

JOB'S METHOD; PROTEIN-PROTEIN COMPLEXES;

PROTEINS;

BASIC FIBROBLAST GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR BB;

ANALYTIC METHOD; COMPARATIVE STUDY; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); FLUORESCENCE SPECTROSCOPY; IMMUNOASSAY; JOB METHOD; MATHEMATICAL ANALYSIS; NOTE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; STOICHIOMETRY; TITRIMETRY;

EID: 0037330983     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00562-6     Document Type: Article

References (5)

1. Eftink M.R. Fluorescence methods for studying equilibrium macromolecule-ligand interactions. Methods Enzymol. 278:1997;221-257.
2. Job P. Recherches sur la formation de complexes minéraux en solution, et sur leur stabilité (Formation and stability of inorganic complexes in solution). Ann. Chim. (Paris). 9:1928;113-203.
3. Ingham K.C. On the application of Job's method of continuous variation to the stoichiometry of protein-ligand complexes. Anal. Biochem. 68:1975;660-663.
4. Huang C.Y. Determination of binding stoichiometry by the continuous variation method: the Job plot. Methods Enzymol. 87:1982;509-525.
5. Russo K., Ragone R., Facchiano A.M., Capogrossi M.C., Facchiano A. Platelet-derived growth factor-BB and basic fibroblast growth factor directly interact in vitro with high affinity. J. Biol. Chem. 277:2002;1284-1291.