Synthesis of antioxidant propyl gallate using tannase from Aspergillus niger van Teighem in nonaqueous media

Bioorganic and Medicinal Chemistry Letters

Volumn 13, Issue 3, 2003, Pages 395-397

  Sharma, Shweta ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIOXIDANT; ENZYME; FOOD ADDITIVE; GALLIC ACID PROPYL ESTER; INFUSORIAL EARTH; TANNASE; TANNIN; UNCLASSIFIED DRUG; WATER;

ARTICLE; DRUG SYNTHESIS; ENZYME IMMOBILIZATION; NONHUMAN; PH; TRANSESTERIFICATION;

ANTIOXIDANTS; ASPERGILLUS NIGER; BUFFERS; CARBOXYLIC ESTER HYDROLASES; CITRATES; CULTURE MEDIA; DIATOMACEOUS EARTH; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; HYDROLYZABLE TANNINS; KINETICS; PROPYL GALLATE; SORBITOL; WATER;

ASPERGILLUS; ASPERGILLUS NIGER;

EID: 0037330573     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(02)00977-0     Document Type: Article

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16. Immobilization of tannase on Celite-545: 5 mg of the tuned tannase powder was dissolved in 5 mL of 100 mM citrate buffer, pH 6.0. This solution was lyophilized. The total solid obtained was dissolved in 300 μL of distilled water. This enzyme solution was added to 100 mg Celite. The thick paste was vortexed, and lyophilzed. The entire solid obtained with 100 mg Celite was used in the reaction.