Heterogeneity of proteolytic enzyme activities in milk samples of different somatic cell count

Journal of Dairy Research

Volumn 70, Issue 1, 2003, Pages 45-50

  Somers, Joanne M ^a   O'Brien, Bernadette ^b   Meaney, William J ^b   Kelly, Alan L ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b ANIMAL AND GRASSLAND RESEARCH AND INNOVATION CENTRE   (Ireland)

Author keywords

Cathepsin; Milk; Plasmin; Proteolysis; Somatic cell count

Indexed keywords

ANIMALS; CATHEPSIN D; CATTLE; CELL COUNT; CYSTEINE ENDOPEPTIDASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; FEMALE; FOOD PRESERVATION; HYDROGEN-ION CONCENTRATION; MILK; MILK PROTEINS; PLASMIN; REGRESSION ANALYSIS;

EID: 0037327217     PISSN: 00220299     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0022029902005988     Document Type: Article

References (28)

1. Andrews AT 1983a Proteinases in normal bovine milk and their action on the caseins. Journal of Dairy Research 50 45-55
2. Andrews AT 1983b Breakdown of caseins by proteinases in bovine milks with high somatic cell counts arising from mastitis or infusion with bacterial endotoxin. Journal of Dairy Research 50 57-66
3. Auldist MJ, Coats S, Sutherland BJ, Mayes JJ, McDowell GH & Rogers GL 1996a Effects of somatic cell count and stage of lactation on raw milk composition and the yield and quality of Cheddar cheese. Journal of Dairy Research 63 269-280
4. Auldist MJ, Coats S, Sutherland BJ, Mayes JJ, McDowell GH & Rogers GL 1996b Effect of somatic cell count and stage of lactation on the quality and storage life of ultra high temperature milk. Journal of Dairy Research 63 377-386
5. Bastian ED, Brown RJ & Ernstrom CA 1991 Plasmin activity and milk coagulation. Journal of Dairy Science 74 3677-3685
6. Bastian ED & Brown RJ 1996 Plasmin in milk and dairy products: an update. International Dairy Journal 6 435-457
7. Blakesley RW & Boezi JA 1977 A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Analytical Biochemistry 82 580-581
8. De Rham O & Andrews AT 1982 The roles of native milk proteinase and its zymogen during proteolysis in normal bovine milk. Journal of Dairy Research 49 577-585
9. Fang W & Sandholm M 1995 Inhibition of proteinase activity in mastitic milk. Journal of Dairy Research 62 61-68
10. Kelly AL, Reid S, Joyce P, Meaney WJ & Foley J 1998 Effect of decreased milking frequency of cows in late lactation on milk somatic cell count, polymorphonuclear leucocyte numbers, composition and proteolytic activity. Journal of Dairy Research 65 365-373
11. Larsen LB & Petersen TE 1995 Identification of five molecular forms of cathepsin D in bovine milk. In Aspartic Proteinases: Structure, Function, Biology and Biomedical Applications, pp. 279-283 (Ed. K Takahashi). New York: Plenum Press
12. Le Roux Y, Colin O & Laurent F 1995 Proteolysis in samples of quarter milk with varying somatic cell counts. 1. Comparison of some indicators of endogenous proteolysis in milk. Journal of Dairy Science 78 1289-1297
13. Lowry OH, Roseborough NL, Lewis Farr A & Randall AJ 1951 Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193 267-275
14. Magboul AAA, Larsen LB, McSweeney PLH & Kelly AL 2001 Cysteine protease activity in bovine milk. International Dairy Journal 11 865-872
15. McSweeney PLH, Fox PF & Olsen NF 1995 Proteolysis of bovine caseins by cathepsin D: preliminary observations and comparison with casein. International Dairy Journal 5 321-336
16. Ng-Kwai-Hang KF, Hayes JF, Moxley JE & Monardes HG 1982 Environmental influences on protein content and composition of bovine milk. Journal of Dairy Science 65 1993-1998
17. O'Brien B, Dillon P, Murphy JJ, Mehra RK, Guinee TP, Connolly JF, Kelly AL & Joyce P 1998 Effects of stocking density and concentrate supplementation of grazing dairy cows on milk production, composition and processing characteristics. Journal of Dairy Research 66 165-176
18. O'Driscoll BM, Rattray FP, McSweeney PLH & Kelly AL 1999 Protease activities in raw milk determined using a synthetic heptapeptide substrate. Journal of Food Science 64 606-611
19. Okigbo LM, Richardson GH, Brown RJ & Ernstrom CA 1985 Coagulation properties of abnormal and normal milk from individual cow quarters. Journal of Dairy Science 68 1893-1896
20. Politis I & Ng-Kwai-Hang KF 1988 Effects of somatic cell counts and milk composition on the coagulating properties of milk. Journal of Dairy Science 71 1740-1746
21. Politis I, Ng-Kwai-Hang KF & Giroux RN 1989a Environmental factors affecting plasmin activity in milk. Journal of Dairy Science 72 1713-1718
22. Politis I, Lachance E, Block E & Turner JD 1989b Plasmin and plasminogen in bovine milk: a relationship with involution? Journal of Dairy Science 72 900-906
23. Richardson BC & Pearse KN 1981 The determination of plasmin in dairy products. New Zealand Journal of Dairy Science and Technology 18 247-252
24. Richardson BC 1983 The proteinases of bovine milk and the effect of pasteurisation on their activity. New Zealand Journal of Dairy Science and Technology 18 233-245
25. Saeman AI, Verdi RJ, Galton DM & Barbano DM 1988 Effect of mastitis on proteolytic activity in bovine milk. Journal of Dairy Science 71 505-512
26. Suzuki J & Katoh N 1990 Cysteine protease in bovine milk capable of hydrolyzing casein as the substrate and elevation of the activity during the course of mastitis. Japanese Journal of Veterinary Science 52 947-954
27. Verdi RJ & Barbano DM 1988 Preliminary investigation of the properties of somatic cell proteases. Journal of Dairy Science 71 534-538
28. White JH, Zavison B, O'Hare K, Gilmore J, Guo MR, Kinstedt P & Politis I 1995 Distribution of plasminogen activator in different fractions of bovine milk. Journal of Dairy Research 62 115-122