Labeling embryonic stem cells with enhanced green fluorescent protein on the hypoxanthineguanine phosphoribosyl transferase locus

Chinese Medical Journal

Volumn 116, Issue 2, 2003, Pages 267-272

  Teng, Lu ^a   Meng, Guoliang ^a   Xing, Yang ^a   Shang, Kegang ^a   Wang, Xiaoke ^b   Gu, Jun ^a  

^a PEKING UNIVERSITY   (China)

^b Beijing Experimental Animal Center   (China)

Author keywords

Embryonic stem cell; Enhanced green fluorescent protein; Homologous recombination; HPRT gene

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL CULTURE; CELL DIFFERENTIATION; CELL LABELING; CELL MANIPULATION; CELL SUSPENSION; CELL TRANSPORT; CELL TYPE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; DEVELOPMENTAL STAGE; DNA VECTOR; ELECTROPORATION; EMBRYO CELL; GENE LOCUS; GENE TARGETING; GENOME; IN VITRO STUDY; KARYOTYPE; MARKER GENE; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; STEM CELL; STEM CELL TRANSPLANTATION;

ANIMALS; CELLS, CULTURED; CHROMOSOME MAPPING; EMBRYO; GREEN FLUORESCENT PROTEINS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; LUMINESCENT PROTEINS; MICE; RECOMBINATION, GENETIC; STEM CELLS; TRANSGENES;

EID: 0037318688     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Hynes M, Rosenthal A. Embryonic stem cells go dopaminergic. Neuron 2000;28:11-14.
2. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981;292:154-156.
3. Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science 1998; 282:1145-1147.
4. Wilder PJ, Kelly D, Brigman K, et al. Inactivation of the FGF-4 gene in embryonic stem cells alters the growth and/or the survival of their early differentiated progeny. Dev Biol 1997;192: 614-629.
5. O'Shea KS. Embryonic stem cell models of development. Anat Rec 1999;257:32-41.
6. Alam J, Cook JL. Reporter genes: application to the study of mammalian gene transcription. Anal Biochem 1990; 188: 245-254.
7. Chalfie M, Tu Y, Euskirchen G, et al. Green fluorescent protein as a marker for gene expression. Science 1994;263:802-805.
8. Welsh S, Kay SA. Reporter gene expression for monitoring gene transfer. Curr Opin Biotechnol. 1997;8:617-622.
9. Zernicka-Goetz M, Pines J, McLean Hunter S, et al. Following cell fate in the living mouse embryo. Development 1997; 124: 1133-1137.
10. Hadjantonakis AK, Gertsenstein M, Ikawa M, et al. Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev 1998;76:79-90.
11. Min JY, Yang Y, Converso KL, et al. Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats. J Appl Physiol 2002;92:288-296.
12. Andressen C, Stocker E, Klinz FJ, et al. Nestin-specific green fluorescent protein expression in embryonic stem cell-derived neural precursor cells used for transplantation. Stem Cells 2001; 19:419-424.
13. Melton DW, Konecki DS, Brennand J, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc Natl Acad Sci U S A 1984; 81:2147-2151.
14. Kuehn MR, Bradley A, Robertson EJ, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 1987;326:295-298.
15. Han R, Chen WS, Tong Y, et al. Establishment of two ES cell lines with good germline contribution. Acta Genet Sinica 1999; 26:208-212.
16. Wassarman PM, DePamphilis ML. Hints on using the guide to techniques in mouse development. Methods Enzymol 1993;225: 33-38.
17. McMahon AP, Bradley A. The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell 1990;62:1073-1085.
18. Palmiter RD, Brinster RL. Germ-line transformation of mice. Annu Rev Genet 1986;20:465-499.
19. Meisler MH. Insertional mutation of 'classical' and novel genes in transgenic mice. Trends Genet 1992;8:341-344.
20. Te Riele H, Maandag ER, Berns A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc Natl Acad Sci U S A 1992;89:5128-5132.
21. Deng C, Capecchi MR. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol Cell Biol 1992;12: 3365-3371.
22. Brustle O, Jones KN, Learish RD, et al. Embryonic stem cell-derived glial precursors: a source of myelinating transplants. Science 1999;285:754-756.
23. McDonald JW, Liu XZ, Qu Y, et al. Transplanted embryonic stem cells survive, differentiate and promote recovery in injured rat spinal cord. Nat Med 1999;5:1410-1412.
24. Liu S, Qu Y, Stewart TJ, et al. Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation. Proc Natl Acad Sci U S A 2000; 97:6126-6131.
25. Tsai M, Wedemeyer J, Ganiatsas S, et al. In vivo immunological function of mast cells derived from embryonic stem cells: an approach for the rapid analysis of even embryonic lethal mutations in adult mice in vivo. Proc Natl Acad Sci U S A 2000;97:9186-9190.