Identifying a protein by MALDI-TOF mass spectrometry: An experiment for the undergraduate laboratory

Journal of Chemical Education

Volumn 80, Issue 2, 2003, Pages 177-180

  Counterman, Anne E ^a   Thompson, Matthew S ^a   Clemmer, David E ^a  

^a INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DATABASE SYSTEMS; DESORPTION; ENZYMES; INFORMATION ANALYSIS; INTERNET; IONIZATION; LASER BEAM EFFECTS; MASS SPECTROMETRY;

PROTEIN SEQUENCING;

EDUCATION;

EID: 0037317098     PISSN: 00219584     EISSN: None     Source Type: Journal    
DOI: 10.1021/ed080p177     Document Type: Article

References (11)

1. Yates, J. R., III J. Mass Spectrom. 1998, 33, 1.
2. Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M. Science 1989, 246, 64.
3. Karas, M.; Hillenkamp, F. Anal. Chem. 1988, 60, 2299-2301.
4. Muddiman, D. C.; Bakhtiar, R.; Hofstadler, S. A.; Smith, R. D. J. Chem. Educ. 1997, 74, 1288.
5. ProteinProspector. http://prospector.ucsf.edu (accessed Sept 2002).
6. For example, see: MASCOT, http://www.matrixscience.com (accessed Sept 2002); MOWSE, http://www.hgmp.mrc.ac.uk/ Bioinformatics/Webapp/mowsel (accessed Sept 2002); and Peptide Search at EMBL http://www.mann.embl-heidelberg.de/Services/PeptideSearch/PeptideSearchIntro.htm l (accessed Sept 2002). The ExPASy Molecular Biology Server, http:// www.expasy.ch (accessed Sept 2002), also provides a compilation of complementary protein analysis tools.
7. For example, see: MASCOT, http://www.matrixscience.com (accessed Sept 2002); MOWSE, http://www.hgmp.mrc.ac.uk/ Bioinformatics/Webapp/mowsel (accessed Sept 2002); and Peptide Search at EMBL http://www.mann.embl-heidelberg.de/Services/PeptideSearch/PeptideSearchIntro.htm l (accessed Sept 2002). The ExPASy Molecular Biology Server, http:// www.expasy.ch (accessed Sept 2002), also provides a compilation of complementary protein analysis tools.
8. For example, see: MASCOT, http://www.matrixscience.com (accessed Sept 2002); MOWSE, http://www.hgmp.mrc.ac.uk/ Bioinformatics/Webapp/mowsel (accessed Sept 2002); and Peptide Search at EMBL http://www.mann.embl-heidelberg.de/Services/PeptideSearch/PeptideSearchIntro.htm l (accessed Sept 2002). The ExPASy Molecular Biology Server, http:// www.expasy.ch (accessed Sept 2002), also provides a compilation of complementary protein analysis tools.
9. For example, see: MASCOT, http://www.matrixscience.com (accessed Sept 2002); MOWSE, http://www.hgmp.mrc.ac.uk/ Bioinformatics/Webapp/mowsel (accessed Sept 2002); and Peptide Search at EMBL http://www.mann.embl-heidelberg.de/Services/PeptideSearch/PeptideSearchIntro.htm l (accessed Sept 2002). The ExPASy Molecular Biology Server, http:// www.expasy.ch (accessed Sept 2002), also provides a compilation of complementary protein analysis tools.
10. Houston, C. T.; Reilly, J. P. Anal. Chem. 1999, 71, 3397.
11. Bruker Daltonik GmbH, http://www.bruker-daltonik.del/products.html (accessed Sept 2002).