Monitoring the production of aflatoxin B1 in wheat by measuring the concentration of nor-1 mRNA

Applied and Environmental Microbiology

Volumn 69, Issue 2, 2003, Pages 1154-1158

  Mayer, Zsuzsanna ^a   Färber, Paul ^b   Geisen, Rolf ^b  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

^b FEDERAL RESEARCH INSTITUTE OF NUTRITION AND FOOD   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AFLATOXINS; GENETIC ENGINEERING; HIGH PRESSURE EFFECTS; LIQUID CHROMATOGRAPHY; RNA;

INCUBATION TIME;

MICROBIOLOGY;

AFLATOXIN B1; BETA TUBULIN; MESSENGER RNA;

GENE EXPRESSION; POLYMERASE CHAIN REACTION; WHEAT;

ARTICLE; ASPERGILLUS FLAVUS; BACTERIAL COUNT; CORRELATION ANALYSIS; FOOD CONTAMINATION; FUNGAL GENE; FUNGUS GROWTH; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NOR 1 GENE; OBSERVATION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TOXIN ANALYSIS; WHEAT;

AFLATOXIN B1; ASPERGILLUS FLAVUS; COLONY COUNT, MICROBIAL; DNA, COMPLEMENTARY; DNA-BINDING PROTEINS; GENE DOSAGE; NUCLEAR PROTEINS; PLANT DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRITICUM; TUBULIN;

ASPERGILLUS; ASPERGILLUS FLAVUS; BACTERIA (MICROORGANISMS); EUKARYOTA; FUNGI; TRITICUM AESTIVUM;

EID: 0037313746     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.2.1154-1158.2003     Document Type: Article

References (23)

1. Brown, M. P., C. S. Brown-Jenco, and G. A. Payne. 1999. Genetic and molecular analysis of aflatoxin biosynthesis. Fungal Genet. Biol. 26:81-98.
2. Doohan, F. M., D. W. Parry, P. Jenkinson, and P. Nicholoson. 1998. The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat. Plant Pathol. 47:197-205.
3. Ellis, W. O., P. J. Smith, B. K. Simpson, S. Khanizadeh, and J. H. Oldham. 1993. Control of growth and aflatoxin production of Aspergillus flavus under modified atmosphere packaging conditions. Food Microbiol. 10:9-21.
4. Färber, P., R. Geisen, and W. H. Holzapfel. 1997. Detection of aflatoxinogenic fungi in figs by a PCR reaction. Int. J. Food Microbiol. 36:215-220.
5. Geisen, R. 1996. A multiplex PCR reaction for the detection of aflatoxin and sterigmatocystin producing fungi. Syst. Appl. Microbiol. 19:388-392.
6. Gourama, H., and L. B. Bullermann. 1995. Antimycotic and antiaflatoxinogenic effect of lactic acid bacteria: a review. J. Food Protect. 57:1275-1280.
7. Häggblom, P. E., and J. Ghosh. 1985. Postharvest production of ochratoxin A by Aspergillus ochraceus and Penicillium viridicatum in barley with different protein levels. Appl. Environ. Microbiol. 49:787-790.
8. Jelinek, C. F., A. E. Pohland, and G. E. Wood. 1989. Worldwide occurrence of mycotoxins in foods and feeds - an update. J. Assoc. Anal. Chem. 72:223-230.
9. Llewellyn, G. C., R. L. Mooney, T. F. Cheatle, and B. Flannigan. 1992. Mycotoxin contamination of spices - an update. Int. Biodeterior. Biodegrad. 29:111-121.
10. Luchese, R. H., and W. F. Harrigan. 1993. Biosynthesis of aflatoxin. The role of nutritional factors. J. Appl. Bacteriol. 74:5-14.
11. Mayer. Z., A. Bagnara, P. Färber, and R. Geisen. 2002. Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real time PCR and its correlation to the cfu of Aspergillus flavus in foods. Int. J. Food Microbiol., in press.
12. Niessen, M. L., and R. F. Vogel. 1998. Group specific PCR detection of potential trichothecene-producing Fusarium species in pure cultures and cereal samples. Syst. Appl. Microbiol. 21:618-631.
13. Pittet, A. 1998. Natural occurrence of mycotoxins in foods and feeds - an updated review. Rev. Vet. Med. 149:479-492.
14. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
15. Schnerr, H., L. Niessen, and R. F. Vogel. 2001. Real time detection of the tri5 gene in Fusarium species by LightCycler™-PCR using SYBR® Green I for continuous fluorescence monitoring. Int. J. Food Microbiol. 71:53-61.
16. Shapira, R., N. Paster, O. Eyal, M. Menasherov, A. Mett, and R. Salomon. 1996. Detection of aflatoxinogenic molds in grains by PCR. Appl. Environ. Microbiol. 62:3270-3273.
17. Stander, M. A., P. S. Steyn, A. Lübben, A. Miljkovic, P. G. Mantle, and G. J. Marais. 2000. Influence of halogen salts on the production of the ochratoxins by Aspergillus ochraceus Wilh. J. Agric. Food Chem. 48:1865-1871.
18. M. J. Sweeney, P. Pamies, and A. D. W. DobsonT. 2000. The use of reverse transcription-polymerase chain reaction (RT-PCR) for monitoring aflatoxin production in Aspergillus parasiticus 439. Int J. Food Microbiol. 56:97-103.
19. Trail, F., P. K. Chang, J. W. Cary, and J. Linz. 1994. Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl. Environ. Microbiol. 60:4078-4085.
20. 1 biosynthetic genes. FEMS Microbiol. Lett. 160:169-176.
21. Xu, H., S. Annis, J. Linz, and F. Trail. 2000. Infection and colonization of peanut pods by Aspergillus parasiticus and the expression of the aflatoxin biosynthetic gene nor-1 in infection hyphae. Physiol. Mol. Plant Pathol. 56:185-196.
22. Yelton, M. M., J. E. Hamer, and W. E. Timberlake. 1984. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc. Natl. Acad. Sci. USA 81:1470-1474.
23. Yu, J., P. K. Chang, J. W. Cary, M. Wright, D. Bhatnagar, T. Cleveland, G. A. Payne, and J. Linz. 1995. Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl. Environ. Microbiol. 61:2365-2371.