Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Journal of Histochemistry and Cytochemistry

Volumn 51, Issue 2, 2003, Pages 259-266

  Yeh, Hung I ^a,b   Lai, Yu Jun ^a   Lee, Yi Nan ^a   Chen, Yi Jen ^b,c   Chen, Yao Chang ^d   Chen, Chih Chun ^a   Chen, Shih Ann ^c   Lin, Cheng I ^d   Tsai, Cheng Ho ^a,b  

^a MACKAY MEMORIAL HOSPITAL   (Taiwan)

^b TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^c NATIONAL YANG MING UNIVERSITY   (Taiwan)

^d NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

Author keywords

Connexins; Gap junctions; Myocardial sleeve; Single cardiomyocytes

Indexed keywords

ALPHA ACTIN; ANTIBODY; CELL MARKER; CONNEXIN 40; CONNEXIN 43; CONNEXIN 43 ANTIBODY; ENZYME; UNCLASSIFIED DRUG; VINCULIN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CONFOCAL MICROSCOPY; DOG; ELECTRIC CONDUCTIVITY; GAP JUNCTION; HEART MUSCLE CELL; IMMUNOCHEMISTRY; MOLECULAR SIZE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PULMONARY VEIN; SUPERIOR CAVA VEIN;

ANIMALIA; CANIS FAMILIARIS; SPRING VIREMIA OF CARP VIRUS;

EID: 0037310449     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540305100215     Document Type: Article

References (27)

1. Chen YJ, Chen SA, Chen YC, Yeh HI, Chan P, Chang MS, Lin CI (2001) Effects of rapid atrial pacing on the arrhythmogenic activity of single cardiomyocytes from pulmonary veins: implication in initiation of atrial fibrillation. Circulation 104:2849-2854
2. Chen YJ, Chen SA, Chen YC, Yeh HI, Chang MS, Lin CI (2002) Electrophysiology of single cardiomyocytes isolated from rabbit pulmonary veins: implication in initiation of focal atrial fibrillation. Basic Res Cardiol 97:26-34
3. Coppen SR, Dupont E, Rothery S, Severs NJ (1998) Connexin45 expression is preferentially associated with the ventricular conduction system in mouse and rat heart. Circ Res 82:232-243
4. Cowan DB, Lye SJ, Langille BL (1998) Regulation of vascular connexin43 gene expression by mechanical loads. Circ Res 82:786-793
5. DePaola N, Davies PF, Pritchard WF, Florez L Jr, Harbeck N, Polacek DC (1999) Spatial and temporal regulation of gap junction connexin43 in vascular endothelial cells exposed to controlled disturbed flows in vitro. Proc Natl Acad Sci USA 96:3154-3159
6. Elfgang C, Eckert R, Lichtenberg-Frate H, Butterweck A, Traub O, Klein RA, Hulser DF, et al. (1995) Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J Cell Biol 129:805-817
7. Gabriels JE, Paul DL (1998) Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed. Circ Res 83:636-643
8. Gros DB, Jongsma HJ (1996) Connexins in mammalian heart function. Bioessays 18:719-730
9. Gutstein DE, Morley GE, Tamaddon H, Vaidya D, Schneider MD, Chen J, Chien KR, et al. (2001) Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43. Circ Res 88:333-339
10. Haissaguerre M, Jais P, Shah DC, Takahashi A, Hocini M, Quiniou G, Garrigue S, et al. (1998) Spontaneous initiation of atrial fibrillation by ectopic beats originating in the pulmonary veins. N Engl J Med 339:659-666
11. Hashizume H, Ushiki T, Abe K (1995) A histological study of the cardiac muscle of the human superior and inferior venae cavae. Arch Histol Cytol 58:457-464
12. Kostin S, Schaper J (2001) Tissue-specific patterns of gap junctions in adult rat atrial and ventricular cardiomyocytes in vivo and in vitro. Circ Res 88:933-939
13. Kwong KF, Schuessler RB, Green KG, Laing JG, Beyer EC, Boineau JP, Saffitz JE (1998) Differential expression of gap junction proteins in the canine sinus node. Circ Res 82:604-612
14. Lerner DL, Yamada KA, Schuessler RB, Saffitz JE (2000) Accelerated onset and increased incidence of ventricular arrhythmias induced by ischemia in Cx43-deficient mice. Circulation 101:547-552
15. Nathan H, Gloobe H (1970) Myocardial atrio-venous junctions and extensions (sleeves) over the pulmonary and caval veins. Anatomical observations in various mammals. Thorax 25:317-324
16. Peters NS, Green CR, Poole-Wilson PA, Severs NJ (1993) Reduced content of connexin43 gap junctions in ventricular myocardium from hypertrophied and ischemic human hearts. Circulation 88:864-875
17. Peters NS, Green CR, Poole-Wilson PA, Severs NJ (1995) Cardiac arrhythmogenesis and the gap junction. J Mol Cell Cardiol 27:37-44
18. Saffitz JE, Davis LM, Darrow BJ, Kanter HL, Laing JG, Beyer EC (1995) The molecular basis of anisotropy: role of gap junctions. J Cardiovasc Electrophysiol 6:498-510
19. Severs NJ, Rothery S, Dupont E, Coppen SR, Yeh H-I, Ko Y-S, Matsushita T, et al. (2001) Immunocytochemical analysis of connexin expression in the healthy and diseased cardiovascular system. Microsc Res Tech 52:301-322
20. Spach MS, Barr RC, Jewett PH (1972) Spread of excitation from the atrium into thoracic veins in human beings and dogs. Am J Cardiol 30:844-854
21. Spach MS, Starmer CF (1995) Altering the topology of gap junctions a major therapeutic target for atrial fibrillation. Cardiovasc Res 30:337-344
22. Tsai CF, Tai CT, Hsieh MH, Lin WS, Yu WC, Ueng KC, Ding YA, et al. (2000) Initiation of atrial fibrillation by ectopic beats originating from the superior vena cava: electrophysiological characteristics and results of radiofrequency ablation. Circulation 102:67-74
23. Vozzi C, Dupont E, Coppen SR, Yeh HI, Severs NJ (1999) Chamber-related differences in connexin expression in the human heart. J Mol Cell Cardiol 31:991-1003
24. Yeh H-I, Chang H-M, Lu W-W, Lee Y-N, Ko Y-S, Severs NJ, Tsai C-H (2000) Age-related alteration of gap junction distribution and connexin expression in rat aortic endothelium. J Histochem Cytochem 48:1377-1389
25. Yeh H-I, Lai Y-J, Lee SH, Lee Y-N, Ko Y-S, Chen SA, Severs NJ, et al. (2001) Heterogeneity of myocardial sleeve morphology and gap junctions in canine superior vena cava. Circulation 104:3152-3157
26. Yeh H-I, Rothery S, Dupont E, Coppen SR, Severs NJ (1998) Individual gap junction plaques contain multiple connexins in arterial endothelium. Circ Res 83:1248-1263
27. Zipes DP, Knope RF (1972) Electrical properties of the thoracic veins. Am J Cardiol 29:372-376