Application of broad-range 16S rRNA PCR amplification and DGGE fingerprinting for detection of tick-infecting bacteria

Journal of Microbiological Methods

Volumn 52, Issue 2, 2003, Pages 251-260

  Schabereiter Gurtner, Claudia ^a   Lubitz, Werner ^a   Rölleke, Sabine ^b  

^a UNIVERSITY OF VIENNA   (Austria)

^b Genalysis GmbH   (Germany)

Author keywords

16S rDNA; Community fingerprinting; DGGE; Ixodes ricinus; PCR

Indexed keywords

DNA 16S; RNA 16S;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; DNA LIBRARY; DNA SCREENING; DNA SEQUENCE; ENDOSYMBIONT; GENE AMPLIFICATION; GENOTYPE; INFESTATION; IXODES RICINUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SPECIES DIFFERENCE;

ANIMALS; BACTERIA; BASE SEQUENCE; CLONING, MOLECULAR; DNA FINGERPRINTING; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IXODES; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

ACANTHAMOEBA; ACANTHAMOEBA SP.; ACARI; ARTHROPODA; BACTERIA (MICROORGANISMS); BARTONELLA; BORRELIA; CANDIDA; COXIELLA; FOLSOMIA; FOLSOMIA CANDIDA; IXODES; IXODES RICINUS; IXODIDA; LEGIONELLACEAE; MYCOPLASMA; PSEUDOMONAS; RHODOCOCCUS; RICKETTSIA; STAPHYLOCOCCUS;

EID: 0037290241     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00186-0     Document Type: Article

References (34)

1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database searchprograms. Nucleic Acids Res. 25:1997;3389-3402.
2. Amoo A.O.J., Dipeolu O.O., Akinboade A.O., Adeyemi A. Bacterial isolation from and transmission by Boophilus decoloratus and Boophilus geigyi. Folia Parasitol. (Praha). 34:1987;69-74.
3. Anderson B.E., Dawson J.E., Jones D.C., Wilson K.H. Ehrlichia chaffeensis, a new species associated with human ehrlichiosis. J. Clin. Microbiol. 29:1991;2838-2842.
4. Duarte M.I.S., Oliveira M.S., Shikanai-Yasuda M.A., Mariano O.N., Takakura C.F.H., Pagliari C., Corbett C.E.P. Haemobartonella-like microorganism infection in AIDS patients: ultrastructural pathology. J. Infect. Dis. 165:1992;976-977.
5. Eskow E., Rao R.V., Mordechai E. Concurrent infection of the central nervous system by Borrelia burgdorferi and Bartonella henselae: evidence for a novel tick-borne disease complex. Arch. Neurol. 58:2001;1357-1363.
6. Farrelly V., Rainey F.A., Stackebrandt E. Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species. Appl. Environ. Microbiol. 61:1995;2798-2801.
7. Ferris M.J., Muyzer G., Ward D.M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl. Environ. Microbiol. 62:1996;340-346.
8. Fritsche T.R., Horn M., Seyedirashti S., Gautom R.K., Schleifer K.-H., Wagner M. In situ detection of novel bacterial endosymbionts of Acanthamoeba spp. phylogenetically related to members of the order Rickettsiales. Appl. Environ. Microbiol. 65:1999;206-212.
9. Gillan D.C., Speksnijder A.G.C.L., Zwart G., De Ridder C. Genetic diversity of the biofilm covering Montacuta ferruginosa (Mollusca, Bivalvia) as evaluated by denaturing gradient gel electrophoresis analysis and cloning of PCR-amplified gene fragments coding for 16S rRNA. Appl. Environ. Microbiol. 64:1998;3464-3472.
10. Hayes S.F., Burgdorfer W. Ultrastructural comparisons of Wolbachia-like symbiotes of ticks (Acari: Ixodidae). Burgdorfer W., Anacker R.L. Rickettsiae and Rickettsial Diseases. 1981;281-289 Academic Press, New York.
11. Hayes S.F., Burgdorfer W. Interactions between rickettsial endocytobionts and their tick hosts. Schwemmler W., Gassner G. Insect Endocyobiosis: Morphology, Physiology, Genetics, Evolution. 1989;235-251 CRC Press, Boca Raton, FL.
12. Head I.M., Saunders J.R., Pickup R.W. Microbial evolution, diversity, and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. Microb. Ecol. 35:1998;1-21.
13. Heuer H., Krsek M., Baker P., Smalla K., Wellington E.M.H. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl. Environ. Microbiol. 63:1997;3233-3241.
14. Hildebrandt A., Schmidt K.-H., Fingerle V., Wilske B., Straube E. Prevalence of granulocytic Ehrlichiae in Ixodes ricinus ticks in Middle Germany (Thuringia) detected by PCR and sequencing of a 16S ribosomal DNA fragment. FEMS Microbiol. Lett. 211:2002;225-230.
15. Hofmeister E.K., Kolbert C.P., Abdukarim A.S. Cosegregation of a novel Bartonella species with Borrelia burgdorferi and Babesia microti in Peromyscus leucopus. J. Infect. Dis. 177:1998;409-416.
16. Jackson C.R., Roden E.E., Churchill P.F. Denaturing gradient gel electrophoresis can fail to separate 16S rDNA fragments with multiple base differences. Mol. Biol. Today. 1:2000;49-51.
17. Johansson K.E., Tully J.G., Bolske G., Pettersson B. Mycoplasma cavipharyngis and Mycoplasma fastidiosum, the closest relatives to Eperythrozoon spp. and Haemobartonella spp. FEMS Microbiol. Lett. 174:1999;321-326.
18. Magnarelli L.A., Stafford K.C. III, Mather T.N., Yeh M.-T., Horn K.D., Dumler J.S. Hemocytic Rickettsia-like organisms in ticks: serologic reactivity with antisera to Ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR. J. Clin. Microbiol. 33:1995;2710-2714.
19. Middendorf L.R., Bruce J.C., Bruce R.C., Eckles R.D., Grone D.L., Roemer S.C., Sloniker G.D., Steffens D.L., Sutter S.L., Brumbaugh J.A., Patonay G. Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus. Electrophoresis. 13:1992;487-494.
20. Murray P.R., Baron E.J., Pfaller M.A., Tenover F.C., Yolken R.H. Manual of Clinical Microbiology. 6th ed. 1995;American Society for Microbiology, Washington, DC.
21. Murray A.E., Hollibaugh J.T., Orrego C. Phylogenetic compositions of bacterioplankton from two Californian estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments. Appl. Environ. Microbiol. 62:1996;2676-2680.
22. Muyzer G., deWaal E.C., Uitterlinden A.G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:1993;695-700.
23. Nilsson K., Lindquist O., Liu A.J., Jaenson T.G.T., Friman G., Påhlson C. Rickettsia helvetica in Ixodes ricinus in Sweden. J. Clin. Microbiol. 37:1999;400-403.
24. Noda H., Munderloh U.G., Kurtti T.J. Endosymbionts of ticks and their relationship to Wolbachia spp. and tick-borne pathogens of humans and animals. Appl. Environ. Microbiol. 63:1997;3926-3932.
25. Pappalardo B.L., Correa M.T., York C.C., Peat C.Y., Breitschwerdt E.B. Epidemiologic evaluation of the risk factors associated with exposure and seroreactivity to Bartonella vinsonii in dogs. Am. J. Vet. Res. 58:1997;467-471.
26. Polz M.F., Cavanaugh C.M. Bias in template-to-product ratios in multitemplate PCR. Appl. Environ. Microbiol. 64:1998;3724-3730.
27. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. 1989; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
28. Schabereiter-Gurtner C., Maca S., Rölleke S., Nigl K., Lukas J., Lubitz W., Barisani-Asenbauer T. 16S rDNA-based identification of bacteria from conjunctival swabs by PCR and DGGE fingerprinting. Investig. Ophthalmol. Vis. Sci. 42:2001;1164-1171.
29. Schouls L.M., VanDe Pol I., Rijpkema S.G.T., Schot C.S. Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks. J. Clin. Microbiol. 37:1999;2215-2222.
30. Seneviratana P., Weerasinghe N., Ariyada S. Transmission of Haemobartonella canis by the dog tick Rhipicephalus sanguineus. Res. Vet. Sci. 14:1973;112-114.
31. Sparagano O.A.E., Allsopp M.T.E.P., Mank R.A., Rijpkema S.G.T., Figueroa J.V., Jongejan F. Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review. Exp. Appl. Acarol. 23:1999;929-960.
32. Stafford K.C. III, Cartter M.L., Magnarelli L.A., Ertel S.-H., Mshar P.A. Temporal correlations between tick abundance and prevalence of ticks infected with Borrelia burgdorferi and increasing incidence of Lyme disease. J. Clin. Microbiol. 36:1998;1240-1244.
33. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol. Rev. 60:1996;407-438.
34. Zhu Z., Aeschlimann A., Gern L. Rickettsia-like microorganisms in the ovarian primordia of molting Ixodes ricinus (Acari: Ixodidae) larvae and nymphs. Ann. Parasitol. Hum. Comp. 67:1992;99-110.