Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against virulence challenge

Veterinary Research

Volumn 34, Issue 1, 2003, Pages 119-125

  Héchard, Céline ^a   Grépinet, Olivier ^a   Rodolakis, Annie ^a  

^a CEMAGREF   (France)

Author keywords

Chlamydophila abortus; DNA immunization; Dnak; Hsp70; Proteic boost

Indexed keywords

ANTIBODY; DNA VACCINE; FREUND ADJUVANT; HEAT SHOCK PROTEIN 70; IMMUNOGLOBULIN G2A; KETAMINE; PROTEIN DNAK; RECOMBINANT PROTEIN; RECOMBINANT VACCINE; XYLAZINE;

ACTIVE IMMUNIZATION; ANTIBODY BLOOD LEVEL; ARTICLE; BACTERIAL GENE; BACTERIAL INFECTION; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BREEDING; CELLULAR IMMUNITY; CHLAMYDOPHILA; CHLAMYDOPHILA ABORTUS; CONTROLLED STUDY; DRUG SCREENING; FEMALE; GRAM NEGATIVE INFECTION; HUMORAL IMMUNITY; IMMUNOSTIMULATION; IN VITRO STUDY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROVOCATION TEST; VACCINATION;

BACTERIA (MICROORGANISMS); CHLAMYDOPHILA ABORTUS; NEGIBACTERIA;

EID: 0037286788     PISSN: 09284249     EISSN: None     Source Type: Journal    
DOI: 10.1051/vetres:2002052     Document Type: Article

References (16)

1. Brunham R.C., Zhang D.J., Yang X., McClarty G.M., The potential for vaccine development against chlamydial infection and disease, J. Infect. Dis. 181 Suppl. 3 (2000) S538-S543.
2. Buzoni-Gatel D., Rodolakis A., Plommet M., T cell mediated and humoral immunity in a mouse Chlamydia psittaci systemic infection, Res. Vet. Sci. 43 (1987) 59-63.
3. Caldwell H.D., Kromhout J., Schachter J., Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis, Infect. Immun. 31 (1981) 1161-1176.
4. Dong-Ji Z., Yang X., Shen C., Lu H., Murdin A., Brunham R.C., Priming with Chlamydia trachomatis major outer membrane protein (MOMP) DNA followed by MOMP ISCOM boosting enhances protection and is associated with increased immumoglobulin A and Th1 cellular immune responses, Infect. Immun. 68 (2000) 3074-3078.
5. Donnelly J.J., Ulmer J.B., Shiver J.W., Liu M.A., DNA vaccines, Annu. Rev. Immunol. 15 (1997) 617-648.
6. Everett K.D.E., Chlamydia and Chlamydiales: more than meets the eye, Vet. Microbiol. 75 (2000) 109-126.
7. Faye P., Charton A., Mage C., Bernard C., Le Layec C., Propriétés hémagglutinantes du "virus" de l'avortement enzootique des petits ruminants (souches de "Rakeia" d'origine ovine et caprine), Note préliminaire, Bull. Acad. Vét. Fr. 45 (1972) 169-173.
8. Héchard C., Grépinet O., Rodolakis A., Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice, Vet. Res. 33 (2002) 313-326.
9. Krieg A.M., Immune effects and mechanisms of action of CpG motifs, Vaccine 19 (2001) 618-622.
10. Penttilä T., Vuola J.M., Puurula V., Anttila M., Sarvas M., Rautonen N., Mäkelä P.H., Puolakkainen M., Immunity to Chlamydia pneumoniae induced by vaccination with DNA vectors expressing a cytoplasmic protein (Hsp60) or outer membrane proteins (MOMP and Omp2), Vaccine 19 (2001) 1256-1265.
11. Raulston J.E., Davis C.H., Paul T.R., Hobbs J.D., Wyrick P.B., Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bonds, Infect. Immun. 70 (2002) 535-543.
12. Rodolakis A., In vitro and in vivo properties of chemically induced temperature-sensitive mutants of Chlamydia psittaci var. ovis: screening in a murine model, Infect. Immun. 42 (1983) 525-530.
13. Rodolakis A., Chancerelle L., [Plaque assay for Chlamydia psittaci in tissue samples (in French)], Ann. Inst. Pasteur Microbiol. B 128 (1977) 81-85.
14. Sin J.I., Bagarazzi M., Pachuk C., Weiner D.B., DNA priming-protein boosting enhances both antigen-specific antibody and Th1-type cellular immune responses in a murine herpes simplex virus-2 gD vaccine model, DNA Cell Biol. 18 (1999) 771-779.
15. Vanrompay D., Cox E., Vandenbussche F., Volckaert G., Goddeeris B., Protection of turkeys against Chlamydia psittaci challenge by gene gun-based DNA immunizations, Vaccine 17 (1999) 2628-2635.
16. Zhang D.-J., Yang X., Berry J., Shen C., McClarty G., Brunham R.C., DNA vaccination with the major outer-membrane protein gene induces acquired immunity to Chlamydia trachomatis (mouse pneumonitis) infection, J. Infect. Dis. 176 (1997) 1035-1040.