Bifunctional Reporter Genes: Construction and Expression in Prokaryotic and Eukaryotic Cells

Molecular Biology

Volumn 37, Issue 2, 2003, Pages 307-313

  Goldenkova, I V ^a   Musiychuk, K A ^a   Piruzian, E S ^a  

^a VAVILOV INSTITUTE OF GENERAL GENETICS   (Russian Federation)

Author keywords

glucuronidase; Bifunctional proteins; Green fluorescent protein; licB; Lichenase; Prokaryotic and eukaryotic transformed cells; Reporter gene

Indexed keywords

BACTERIAL ENZYME; BETA GLUCURONIDASE; GREEN FLUORESCENT PROTEIN; LICHENASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CELL TRANSFORMATION; CLOSTRIDIUM THERMOCELLUM; CONTROLLED STUDY; ESCHERICHIA COLI; EUKARYOTIC CELL; FUNGAL CELL; FUNGAL GENE; GENE CONSTRUCT; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE SEQUENCE; GENETIC CONSERVATION; MAMMAL; MAMMAL CELL; MAMMALIAN GENETICS; NONHUMAN; PRESERVATION; PROKARYOSIS; PROTEIN FUNCTION; REPORTER GENE; SACCHAROMYCES CEREVISIAE;

AEQUOREA; AEQUOREA VICTORIA; ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI; EUKARYOTA; MAMMALIA; PROKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; VICTORIA;

EID: 0037272868     PISSN: 00268933     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1023310124842     Document Type: Article

References (21)

1. Patrushev L.I. Ekspressiya genov (Gene expression). Moscow: Nauka. 2000.
2. Rutter G.A., Kennedy H.J., Wood C.D., White M.R.H., Tavare J.M. 1998. Real-time imaging of gene expression in single cells. Chem. Biol. 5, R285-R290.
3. Naylor L.H. 1999. Reporter gene technology: The future looks bright. Biochem. Pharmacol. 58, 749-757.
4. Alam J., Cook J.L. 1990. Reporter genes: application to the study of mammalian gene transcription. Analit. Biochem. 188, 245-254.
5. Piruzian E.S., Monzavi-Karbassi B., Darbinian N.S., Goldenkova I.V., Kobets N.S., Mochulsky A.V. 1998. The use of a thermoactive β-glucanase gene from Clostridium thermocellum as a reporter gene in plants. Mol. Gen. Genet. 257, 561-567.
6. Goldenkova I.V., Musiychuk K.A., Piruzian E.S. 2002. A thermostable Clostridium thermocellum lichenase-based reporter system for studying the gene expression regulation in prokaryotic and eukaryotic cells. Mol. Biol. 36, 1-9.
7. Piruzian E.S., Goldenkova I.V., Musiychuk K.A., Abdeev R.M., Volkova L.V., Kobets N.S. 2000. A new thermostable lichenase-based reporter system for studying the gene expression regulation in plants. Fiziol. Rast. 37, 327-336.
8. Piruzian E.S., Goldenkova I.V., Mysiychuk K.A., Kobets N.S., Arman I.P., Bobrysheva I.V., Chekhuta I.F., Glazkova D. 2002. A reporter system for prokaryotic and eukaryotic cells based on the thermostable lichenase from Clostridium thermocellum. Mol. Genet. Gen. 266, 778-786.
9. Quaedvlieg N.E.M., Schlaman H.R.M., Admiraal P.C., Wijting S.E., Stougaard J., Spaink H.P. 1998. Fusions between green fluorescent protein and beta-glucuronidase as sensitive and vital bifunctional reporters in plants. Plant Mol. Biol. 38, 861-873.
10. Zhang J.X., Flint H.J. 1992. A bifunctional xylanase encoded by the xynA gene of the rumen cellulolytic bacterium Ruminococcus flavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence. Mol. Microbiol. 6, 1013-1023.
11. Flint H.J., Martin J., McPherson C.A., Daniel A.S., Zhang J.X. 1993. A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. J. Bacteriol. 175, 2943-2951.
12. Olsen O., Thomsen K.K., Weber J., Duus J.O., Svendsen I., Wegener C., von Wettstein D. 1996. Transplanting two unique beta-glucanase catalytic activities into one multienzyme, which forms glucose. Biotechnol. (N.Y.). 14, 71-76.
13. Green L.A., Tischler A. 1976. Establishment of a nonadrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA. 73, 2424-2428.
14. Musiychuk K.A., Goldenkova I.V., Abdeev R.M., Kobets N.S., Piruzian E.S. 2000. Construction and properties of deletion derivatives of Clostridium thermocellum lichenase and their use to obtain bifunctional hybrid proteins. Biokhimiya. 65, 1659-1665.
15. Jefferson R.A., Kavanagh T.A., Bevan M.W. 1987. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901-3907.
16. Soni R., Carmichael J.P., Murray J.A. 1993. Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure. Curr. Genet. 24, 455-459.
17. Bradford M.M. 1976. A rapid sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analit. Biochem. 72, 248-254.
18. Jefferson R.A., Burgess S.M., Hirsh D. 1986. β-glucoronidase from Escherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. USA. 83, 8551-8557.
19. Schimming S., Schwarz W.H., Staudenbauer W.L. 1992. Structure of the Clostridium thermocellum gene licB and the encoded β-1,3-1,4-glucanase. A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases. Eur. J. Biochem. 204, 13-19.
20. Strathdee C.A., McLeod M.R., Underhill T.M. 2000. Dominant positive and negative selection using luciferase, green fluorescent protein and β-galactosidase reporter gene fusions. BioTechniques. 28, 210-214.
21. Datla R.S.S., Hammerlindl J.K., Pelcher L.E., Crosby W.L., Selvaraj G. 1991. A bifunctional fusion between β-glucoronidase and neomycin phosphotransferase: a broad-spectrum marker enzyme for plants. Gene. 101, 239-246.