Development and clinical application of a fully controlled quantitative PCR assay for cell-free cytomegalovirus in human plasma

Journal of Clinical Virology

Volumn 26, Issue 1, 2003, Pages 49-59

  Preiser, Wolfgang ^a,c   Brink, Nicola S ^a   Ayliffe, Ursula ^a   Peggs, Karl S ^b   Mackinnon, Stephen ^b   Tedder, Richard S ^a   Garson, Jeremy A ^a  

^a ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY HOSPITAL FRANKFURT   (Germany)

Author keywords

Bone marrow transplant; Genome quantification; Human cytomegalovirus; Pre emptive antiviral therapy; Quantitative polymerase chain reaction

Indexed keywords

VIRUS DNA;

AMPLICON; ANTIMICROBIAL THERAPY; ARTICLE; BLOOD ANALYSIS; BLOOD STORAGE; BONE MARROW TRANSPLANTATION; CONTROL; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOMEGALOVIRUS INFECTION; DIAGNOSTIC ACCURACY; DNA DETERMINATION; DNA EXTRACTION; DRUG WITHDRAWAL; EVALUATION; GENE AMPLIFICATION; HUMAN; HUMAN CYTOMEGALOVIRUS; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NONHUMAN; PLASMA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; VIRUS CULTURE; VIRUS DIAGNOSIS; VIRUS LOAD;

BASE SEQUENCE; BLOOD PRESERVATION; BONE MARROW TRANSPLANTATION; CHEMILUMINESCENT MEASUREMENTS; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; FALSE NEGATIVE REACTIONS; FOLLOW-UP STUDIES; HUMANS; IMMUNOCOMPROMISED HOST; MOLECULAR SEQUENCE DATA; MUROMEGALOVIRUS; PLASMA; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; TIME FACTORS; TRANSPLANTATION, HOMOLOGOUS; VIRAL LOAD; VIREMIA;

EID: 0037270685     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1386-6532(02)00022-7     Document Type: Article

References (34)

1. Berger A., Preiser W., Doerr H.W. The role of viral load determination for the management of human immunodeficiency virus, hepatitis B virus and hepatitis C virus infection. J. Clin. Virol. 20:2001;23-30.
2. Boeckh M., Bowden R.A., Goodrich J.M., Pettinger M., Meyers J.D. Cytomegalovirus antigen detection in peripheral blood leukocytes after allogeneic marrow transplantation. Blood. 80:(5):1992;1358-1364.
3. Boeckh M., Myerson D., Bowden R.A. Early detection and treatment of cytomegalovirus infections in marrow transplant patients: methodological aspects and implications for therapeutic interventions. Bone Marrow Transplant. 14:(Suppl 4):1994;S66-S70.
4. Boeckh M., Gallez-Hawkins G.M., Myerson D., Zaia J.A., Bowden R.A. Plasma polymerase chain reaction for cytomegalovirus DNA after allogeneic marrow transplantation: comparison with polymerase chain reaction using peripheral blood leukocytes, pp65 antigenemia, and viral culture. Transplantation. 64:(1):1997;108-113.
5. Boeckh M., Boivin G. Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clin. Microbiol. Rev. 11:(3):1998;533-554.
6. Boom R., Sol C., Weel J., Gerrits Y., de Boer M., Wertheim-van Dillen P. A highly sensitive assay for detection and quantitation of human cytomegalovirus DNA in serum and plasma by PCR and electrochemiluminescence. J. Clin. Microbiol. 37:1999;1489-1497.
7. Bowen E.F., Wilson P., Cope A., Sabin C., Griffiths P., Davey C., et al. Cytomegalovirus retinitis in AIDS patients: influence of cytomegaloviral load on response to ganciclovir, time to recurrence and survival. AIDS. 10:(13):1996;1515-1520.
8. de Jong, MD, Galasso GJ, Gazzard B, Griffiths PD, Jabs DA, Kern ER, et al. Mini review: Summary of the II International Symposium on Cytomegalovirus. Antiviral Res 1998; 39:141-162.
9. Einsele H., Ehninger G., Hebart H., Wittkowski K.M., Schuler U., Jahn G., et al. Polymerase chain reaction monitoring reduces the incidence of cytomegalovirus disease and the duration and side effects of antiviral therapy after bone marrow transplantation. Blood. 86:(7):1995;2815-2820.
10. Emery V.C., Sabin C.A., Cope A.V., Gor D., Hassan-Walker A.F., Griffiths P.D. Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation. Lancet. 355:2000;2032-2036.
11. Fox J.C., Kidd I.M., Griffiths P.D., Sweny P., Emery V.C. Longitudinal analysis of cytomegalovirus load in renal transplant recipients using a quantitative polymerase chain reaction: correlation with disease. J. Gen. Virol. 76:1995;309-319.
12. Gerdes J.C., Spees E.K., Fitting K., Hiraki J., Sheehan M., Duda D., et al. Prospective study utilizing a quantitative polymerase chain reaction for detection of cytomegalovirus DNA in the blood of renal transplant patients. Transplant. Proc. 25:(1 Part 2):1993;1411-1413.
13. Gerna G., Furione M., Baldanti F., Sarasini A. Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J. Clin. Microbiol. 32:(11):1994;2709-2717.
14. Gerna G., Percivalle E., Baldanti F., Sarasini A., Zavattoni M., Furione M., et al. Diagnostic significance and clinical impact of quantitative assays for diagnosis of human cytomegalovirus infection/disease in immunocompromised patients. Microbiologica. 21:1998;293-308.
15. Goodrich J.M., Mori M., Gleaves C.A., Du Mond C., Cays M., Ebeling D.F., et al. Early treatment with ganciclovir to prevent cytomegalovirus disease after allogeneic bone marrow transplantation. N. Engl. J. Med. 325:(23):1991;1601-1607.
16. Goodrich J.M., Bowden R.A., Fisher L., Keller C., Schoch G., Meyers J.D. Ganciclovir prophylaxis to prevent cytomegalovirus disease after allogeneic marrow transplant. Ann. Intern. Med. 118:(3):1993;173-178.
17. Gor D., Sabin C., Prentice H.G., Vyas N., Man S., Griffiths P.D., et al. Longitudinal fluctuations in cytomegalovirus load in bone marrow transplant patients: relationship between peak virus load, donor/recipient serostatus, acute GVHD and CMV disease. Bone Marrow Transplant. 21:(6):1998;597-605.
18. Griffiths PD, Clark DA, Emery VC. Betaherpesviruses in transplant recipients. J Antimicrob Chemother 2000; Suppl T3, 29-34.
19. Hamprecht K., Steinmassl M., Einsele H., Jahn G. Discordant detection of human cytomegalovirus DNA from peripheral blood mononuclear cells, granulocytes and plasma: correlation to viremia and HCMV infection. J. Clin. Virol. 11:(2):1998;125-136.
20. Meyers J.D., Flournoy N., Thomas E.D. Nonbacterial pneumonia after allogeneic marrow transplantation: a review of ten years' experience. Rev. Infect. Dis. 4:(6):1982;1119-1132.
21. Mitchell S.M., Fox J.D., Tedder R.S., Gazzard B.G., Lightman S. Vitreous fluid sampling and viral genome detection for the diagnosis of viral retinitis in patients with AIDS. J. Med. Virol. 43:(4):1994;336-340.
22. Nichols W.G., Boeckh M. Recent advances in the therapy and prevention of CMV infections. J. Clin. Virol. 16:(1):2000;25-40.
23. Peggs K.S., Preiser W., Kottaridis P.D., McKeag N., Brink N.S., Tedder R.S., et al. Extended routine polymerase chain reaction surveillance and pre-emptive antiviral therapy for cytomegalovirus after allogeneic transplantation. Br. J. Haematol. 111:2000;782-790.
24. Preiser W., Elzinger B., Brink N.S. Quantitative molecular virology in patient management. J. Clin. Pathol. 53:(1):2000;76-83.
25. Preiser W., Bräuninger S., Schwerdtfeger R., Ayliffe U., Garson J.A., Brink N.S., et al. Evaluation of diagnostic methods for the detection of cytomegalovirus in recipients of allogeneic stem cell transplants. J. Clin. Virol. 20:2001;59-70.
26. Schmidt G.M., Horak D.A., Niland J.C., Duncan S.R., Forman S.J., Zaia J.A. A randomized, controlled trial of prophylactic ganciclovir for cytomegalovirus pulmonary infection in recipients of allogeneic bone marrow transplants; The City of Hope-Stanford-Syntex CMV Study Group. N. Engl. J. Med. 324:(15):1991;1005-1011.
27. Selgrade M.K., Nedrud J.G., Collier A.M., Gardner D.E. Effects of cell source, mouse strain, and immunosuppressive treatment on production of virulent and attenuated murine cytomegalovirus. Infect. Immun. 33:(3):1981;840-847.
28. Simmonds P., Zhang L.Q., Watson H.G., Rebus S., Ferguson E.D., Balfe P., et al. Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users. Lancet. 336:(8729):1990;1469-1472.
29. Spector S.A., Wong R., Hsia K., Pilcher M., Stempien M.J. Plasma cytomegalovirus (CMV) DNA load predicts CMV disease and survival in AIDS patients. J. Clin. Invest. 101:(2):1998;497-502.
30. Taylor-Wiedeman J., Sissons J.G., Borysiewicz L.K., Sinclair J.H. Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells. J. Gen. Virol. 72:1991;2059-2064.
31. Webster A., Blizzard B., Pillay D., Prentice H.G., Pothecary K., Griffiths P.D. Value of routine surveillance cultures for detection of CMV pneumonitis following bone marrow transplantation. Bone Marrow Transplant. 12:(5):1993;477-481.
32. Whitby K., Garson J.A. Optimisation and evaluation of a quantitative chemiluminescent polymerase chain reaction assay for hepatitis C virus RNA. J. Virol. Meth. 51:(1):1995;75-88.
33. Winston D.J., Ho W.G., Bartoni K., Du Mond C., Ebeling D.F., Buhles W.C., et al. Ganciclovir prophylaxis of cytomegalovirus infection and disease in allogeneic bone marrow transplant recipients. Results of a placebo-controlled, double-blind trial. Ann. Intern. Med. 118:(3):1993;179-184.
34. Zaia J.A., Forman S.J. Cytomegalovirus infection in the bone marrow transplant recipient. Infect. Dis. Clin. North Am. 9:(4):1995;879-900.