Spectroscopic and kinetic characterization of active site mutants of Desulfovibrio fructosovorans Ni-Fe hydrogenase

Journal of Biological Inorganic Chemistry

Volumn 8, Issue 1-2, 2003, Pages 129-134

  DeLacey, Antonio L ^a   Fernandez, Victor M ^a   Rousset, Marc ^b   Cavazza, Christine ^b   Hatchikian, E Claude ^b  

^a CSIC   (Spain)

^b CNRS   (France)

Author keywords

FTIR; Hydrogen; Metalloprotein; Site directed mutagenesis; Spectroelectrochemistry

Indexed keywords

HYDROGENASE; IRON SULFUR PROTEIN; NICKEL IRON HYDROGENASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CRYSTALLOGRAPHY; DESULFOVIBRIO FRUCTOSOVORANS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME STRUCTURE; HYDROGEN BOND; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATION REDUCTION STATE; POINT MUTATION; POTENTIOMETRY; PRIORITY JOURNAL; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; SPECTROSCOPY;

AMINO ACIDS; BINDING SITES; CATALYSIS; CYANIDES; DESULFOVIBRIO; HYDROGEN BONDING; HYDROGENASE; KINETICS; LIGANDS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TEMPERATURE; TITRIMETRY;

DESULFOVIBRIO; DESULFOVIBRIO FRUCTOSOVORANS;

EID: 0037257457     PISSN: 09498257     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00775-002-0397-4     Document Type: Article

References (24)

1. Volbeda A, Charon MH, Piras C, Hatchikian EC, Frey M, Fontecilla-Camps JC (1995) Nature 373:580-587
2. Volbeda A, Garcin E, Piras C, De Lacey AL, Fernandez VM, Hatchikian EC, Frey M, Fontecilla-Camps JC (1996) J Am Chem Soc 118:12,989-12,996
3. Happe RP, Roseboom W, Pierik AJ, Albracht SPJ, Bagley KA (1997) Nature 385:126
4. Bagley KA, Van Garderen CJ, Chen M, Duin EC, Albracht SPJ, Woodruff WH (1994) Biochemistry 33:9229-9236
5. Bagley KA, Duin EC, Roseboom W, Albracht SPJ, Woodruff WH (1995) Biochemistry 34:5527-5535
6. De Lacey AL, Hatchikian EC, Volbeda A, Frey M, Fontecilla-Camps JC, Fernandez VM (1997) J Am Chem Soc 119:7181-7189
7. Carepo M, Tierney DL, Brondino CD, Yang TC, Pamplona A, Telser J, Moura I, Moura JJG, Hoffman BM (2002) J Am Chem Soc 124:281-286
8. Higuchi Y, Ogata H, Miki K, Yasuoka N, Yagi T (1999) Structure 7:549-556
9. Garcin E, Vernede X, Hatchikian EC, Volbeda A, Frey M, Fontecilla-Camps JC (1999) Structure 7:557-566
10. Davidson G, Choudhury SB, Gu Z, Bose K, Roseboom W, Albracht SPJ, Maroney MJ (2000) Biochemistry 39:7468-7479
11. Moura JJG, Moura I, Huyhn BH, Kruger HJ, Teixeira M, DuVarney RC, DerVartanian DV, Xavier AV, Peck HD Jr, LeGall J (1982) Biochem Biophys Res Commun 108:1388-1393
12. Cammack R, Patil DS, Hatchikian EC, Fernandez VM (1987) Biochim Biophys Acta 912:98-109
13. Montet Y, Amara P, Volbeda A, Vernede X, Hatchikian EC, Field MJ, Frey M, Fontecilla-Camps JC (1997) Nat Struct Biol 4:523-526
14. Hatchikian EC, Traore AS, Fernandez VM, Cammack R (1990) Eur J Biochem 187:635-643
15. De Lacey AL, Stadler C, Fan H, Li S, Hall MB, Hatchikian EC, Fernandez VM (2002) J Biol Inorg Chem 7:318-326
16. Rousset M, Montet Y, Guigliarelli B, Forget N, Asso M, Bertrand P, Fontecilla-Camps JC, Hatchikian EC (1998) Proc Nat Acad Sci USA 95:11,625-11,630
17. Rousset M, Casalot L, Gilles BJ, Dermoun Z, de Philip P, Bélaich JP, Wall JD (1998) Plasmid 39:114-122
18. Rousset M, Dermoun Z, Chippaux M, Bélaich JP (1991) Molec Microbiol 5:1735-1740
19. Higuchi R, Krummel B, Saiki RK (1988) Nucleic Acids Res 16:7351-7367
20. Fernandez VM, Hatchikian EC, Cammack R (1985) Biochim Biophys Acta 832:69-79
21. Coremans JMCC, van der Zwaan JW, Albracht SPJ (1992) Biochim Biophys Acta 1119:157-168
22. Lai C-H, Lee W-Z, Miller ML, Reibenspies JH, Darensbourg DJ, Darensbourg MY (1998) J Am Chem Soc 120:10,103-10, 114
23. Darensbourg MY, Lyon EJ, Smee JJ (2000) Coord Chem Rev 206/207:533-561
24. Page CC, Moser CC, Chen X, Dutton L (1999) Nature 402:47-52