Infrared-spectroelectrochemical characterization of the [NiFe] hydrogenase of Desulfovibrio gigas

Journal of the American Chemical Society

Volumn 119, Issue 31, 1997, Pages 7181-7189

  De Lacey, Antonio L ^a   Hatchikian, E Claude ^b   Volbeda, Anne ^c   Frey, Michel ^c   Fontecilla Camps, Juan Carlos ^c   Fernandez, Victor M ^a  

^a CSIC   (Spain)

^b CNRS   (France)

^c INSTITUT DE BIOLOGIE STRUCTURALE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGENASE;

ARTICLE; DESULFOVIBRIO; ELECTROCHEMISTRY; ENZYME STRUCTURE; INFRARED SPECTROSCOPY; INTERMETHOD COMPARISON; OXIDATION REDUCTION POTENTIAL; OXIDATION REDUCTION STATE; TITRIMETRY;

EID: 0030805822     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja963802w     Document Type: Article

References (41)

1. Hatchikian, E. C.; Bruschi, M.; LeGall, J. Biochem. Biophys. Res. Commun. 1978, 82, 451-461.
2. Cammack, R.; Patil, D.; Aguirre, R.; Hatchikian, E. C. FEBS Lett. 1982, 142, 289-292.
3. LeGall, J.; Ljungdahl, P. O.; Moura, I.; Peck, H. D.; Xavier, A.; Moura, J. J. G.; Teixeira, M.; Huyn, B. H.; DerVartanian, D. V. Biochem. Biophys. Res. Commun. 1982, 106, 610-615.
4. Krüger, H. J.; Huynh, B. H.; Ljungdahl, P. O.; Xavier, A. V.; DerVartanian, D. V.; Moura, I.; Peck, H. D., Jr.; Texeira, M.; Moura, J. J. G.; LeGall, J. J. Biol. Chem. 1982, 257, 14620-14623.
5. Volbeda, A.; Charon, M. H.; Piras, C.; Hatchikian, E. C.; Frey, M.; Fontecilla-Camps, J. C. Nature 1995, 373, 580-587.
6. Volbeda, A.; Garcin, E.; Piras, C.; de Lacey, A. L.; Fernandez, V. M.; Hatchikian, E. C.; Frey, M.; Fontecilla-Camps, J. C., J. Am. Chem. Soc. 1996, 118, 12989-12996.
7. Fernandez, V. M.; Hatchikian, E. C.; Cammack, R. Biochim. Biophys. Acta 1985, 832, 69-79.
8. Fernandez, V. M.; Hatchikian, E. C.; Paul, D. S.; Cammack, R. Biochim. Biophys. Acta 1986, 883, 145-154.
9. Cammack, R.; Paul, D. S.; Hatchikian, E. C.; Fernández, V. M. Biochim. Biophys. Acta 1987, 912, 98-109.
10. Moura, J. J. G.; Moura, I.; Huyhn, B. H.; Krüger, H. J.; Teixeira, M.; DuVarney, R. C.; DerVartanian, D. V.; Xavier, A. V.; Peck, H. D., Jr.; LeGall, J. Biochem. Biophys. Res. Commun. 1982, 108, 1388-1393.
11. Roberts, L. M.; Lindahl, P. A. Biochemistry 1994, 33, 14339-14349.
12. Roberts, L. M.; Lindahl, P. A. J. Am. Chem. Soc. 1995, 117, 2565-2572.
13. Teixeira, M., Moura, I.; Xavier, A. V.; DerVartanian, D. V.; LeGall, J.; Peck, H. D., Jr.; Huyhn, B. H.; Moura, J. J. G. Eur. J. Biochem. 1983, 130, 481-484.
14. Albracht, S. P. J. Biochim. Biophys. Acta, 1994, 1188, 167-204.
15. Bagley, K. A.; Van Garderen, C. J.; Chen, M.; Duin, E. C.; Albracht, S. P. J.; Woodruff, W. H. Biochemistry 1994, 33, 9229-9236.
16. Bagley, K. A.; Duin, E. C.; Roseboom, W.; Albracht, S. P. J.; Woodruff, W. H. Biochemistry 1995, 34, 5527-5535.
17. Happe, R. P.; Roseboom, W.; Pierik, A. J.; Albracht, S. P. J.; Bagley, K. A. Nature 1997, 385, 126.
18. Teixeira, M., Moura, I.; Xavier, A. V.; Moura, J. J. G.; LeGall, J.; DerVartanian, D. V.; Peck, H. D., Jr.; Huyhn, B. H. J. Biol. Chem. 1989, 264, 16435-16450.
19. Huang, Y. H.; Park, J. B.; Adams, M. W. W.; Johnson, M. K. Inorg. Chem. 1993, 32, 375-376.
20. Bagyinka, C.; Whitehead, J. P.; Maroney, M. J. J. Am. Chem. Soc. 1993, 115, 3576-3585.
21. Cammack, R.; Fernandez, V. M.; Hatchikian, E. C. Methods Enzymol. 1994, 243, 43-68.
22. Moss, D.; Nabedryk, E.; Breton, J.; Mäntele, W. Eur. J. Biochem. 1990, 187, 565-572.
23. Although all three bands of each redox state titrate simultaneously, only the one with lowest frequency is taken into acount as it is much more intense than the other two and because some states have in common the frequency of one of the less intense bands.
24. 1914 could not be obtained because the enzyme was not stable at pH 10.5.
25. Van der Spek, T. M.; Arendsen, A. F.; Happe, R. P.; Yun, S.; Bagley, K. A.; Stufkens, D. J.; Hagen, W. R.; Albracht, S. P. J. Eur. J. Biochem. 1996, 237, 629-634.
26. Nakamoto, K. In Infrared and Raman Spectroscopy of Inorganic and Coordination Compounds; John Wiley & Sons; New York, 1986.
27. Onaka, S.; Furuta, H.; Takagi, S. Angew. Chem., Int. Ed. Engl. 1993, 32, 87-88.
28. Scott, R. A.; Wallin, S. A.; Czechowski, M.; DerVartanian, D. V.; LeGall, J.; Peck, H.; Moura, I. J. Am. Chem. Soc. 1984, 106, 6864-6865.
29. Gu, Z.; Dong, J.; Allan, C. B.; Choudhury, S. B.; Franco, R.; Moura, J. J. G.; Moura I.; LeGall, J.; Przybyla A. E.; Roseboom, W.; Albracht, S. P. J., Axley, M.J.; Scott, R. A.; Maroney, M. J. J. Am. Chem. Soc. 1996, 118, 11155-11165.
30. Lissolo, T.; Pulvin, S.; Thomas, T. J. Biol. Chem. 1984, 259, 11725-11730.
31. Fersht, A. In Enzyme Structure and Mechanism; W. H. Freeman and Company: San Francisco, 1977; p 2.
32. Barondeau, D. P.; Roberts, L. M.; Lindahl, P. A. J. Am. Chem. Soc. 1994, 116, 3442-3448.
33. Mege, R. M.; Bourdillon, C. J. Biol. Chem. 1985, 260, 14701-14706.
34. Although the IR-spectroelectrochemical cell is not completely gas tight, the oxygen that diffuses into the cell is quickly reduced by the redox mediators present in the sample, therefore anaerobic conditions are maintained.
35. Coremans, J. M. C. C.; van der Zwaan, J. W.; Albracht, S. P. J. Biochim. Biophys. Acta 1992, 1119, 157-168.
36. m at pH = 8.0 shown in this scheme have been calculated from those of Table 2 by taking into account their dependence of the pH, as determined in this work The states for which Ni is EPR-detectable are marked with an asterisk.
37. The electron balance of SU conversion to SI form has not yet been quantified.
38. Teixeira, M.; Moura, I.; Xavier, A. V.; Huynh, B. H.; DerVartanian, D. V.; Peck, H. D., Jr.; LeGall, J.; Moura, J. J. G. J. Biol. Chem. 1985, 260, 8942-8950.
39. Fontecilla-Camps, J. C. JBIC 1996, 1, 91-98.
40. Fan, C.; Teixeira, M.; Moura, J.; Moura, I.; Huynh, B. H.; LeGall, J.; Peck, H. D., Jr.; Hoffman, B. M. J. Am. Chem. Soc. 1991, 113, 20-24.
41. Kraulis, P. J. Appl. Crystallogr. 1991, 24, 949-950.