Effect of various stabilizing agents on Imperata cylindrica grass pollen allergen extract

Clinical and Experimental Allergy

Volumn 33, Issue 1, 2003, Pages 65-71

  Bijli, K M ^a   Singh, B P ^a   Sridhara, S ^a   Gaur, S N ^b   Arora, Naveen ^a,c  

^a UNIVERSITY OF DELHI   (India)

^b UNIVERSITY OF DELHI   (India)

^c CSIR INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY   (India)

Author keywords

aminocaproic acid; Pollen extract; Preservative; Protease inhibitor; Stability; Storage

Indexed keywords

AMINOCAPROIC ACID; GLYCEROL; HUMAN SERUM ALBUMIN; IMMUNOGLOBULIN E; PHENOL; POLLEN EXTRACT; PROTEIN; STABILIZING AGENT; SUCROSE;

ANTIGEN BINDING; ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GRASS POLLEN; HUMAN; HUMAN TISSUE; IMPERATA CYLINDRICA; MAJOR CLINICAL STUDY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRESERVATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; QUALITY CONTROL; ROOM TEMPERATURE; SKIN TEST; STANDARD; WESTERN BLOTTING;

ALLERGENS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXCIPIENTS; HUMANS; HYPERSENSITIVITY; IMMUNOBLOTTING; IMMUNOGLOBULIN E; PLANT EXTRACTS; POACEAE; POLLEN; SKIN TESTS; SPECIMEN HANDLING; TIME FACTORS;

EID: 0037244519     PISSN: 09547894     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2222.2003.01554.x     Document Type: Article

References (19)

1. Nelson HS, Ikle D, Buckmeier A. Studies on allergen extract stability: the effects of dilution and mixing. J Allergy Clin Immunol 1996; 98:382-8.
2. Hoff M, Krail M, Kastner M, Haustein D, Vieths S. Fusarium culmorum causes strong degradation of pollen allergens in extract mixtures. J Allergy Clin Immunol 2002; 109:96-101.
3. Hoeyveld EM, Erik AM, Stevens MD. Stabilizing effect of epsilonaminocaproic on allergenic extracts. J Allergy Clin Immunol 1985; 76:543-50.
4. Cadot P, Lejoly M, Stevens EA. The effect of sucrose on the quality of rye grass pollen extracts. Allergy 1995; 50:941-51.
5. Hoffman VS, Holzhauser T, Muller U, Reindl J, Haustein D. Factors influencing the quality of food extracts for in vitro and in vivo diagnosis. Allergy 1998; 53:65-71.
6. Saldotova LN, Paupore E J, Burk SH, Pastor RW, Slater JE. The stability of house dust mite allergens in glycerinated extracts. J Allergy Clin Immunol 2000; 105:482-8.
7. Sridhara S, Singh BP, Arora N, Verma J, Gangal SV. A study on antigenic changes during storage in three different biological extracts. Asian Pacific J Allergy Immunol 1992; 10:33-8.
8. Naerdal Vilsvik JS. Stabilization of a diluted aqueous mite allergen preparation by addition of human serum albumin. An intracutaneous test study. Clin Allergy 1983; 13:149-53.
9. Sridhara S, Singh BP, Kumar L, Verma J, Gaur SN, Gangal SV. Antigenic and allergenic relationships among airborne grass pollens in India. Ann Allergy Asthma Immunol 1995; 75:73-9.
10. Kumar L, Sridhara S, Singh BP, Gangal SV. Characterization of the cogon grass (Imperata cylindrica) pollen extract and preliminary analysis of grass group 1,4 and 5 homologues using monoclonal antibodies to Phleum Pratense. Int Arch Allergy Immunol 1998; 117:174-9.
11. Verma J, Singh BP, Gangal SV, Arora N, Sridhara S. Purification and partial characterization of a 67 kDa cross-reactive allergen from Imperata cylindrica pollen extract. Int Arch Allergy Immunol 2000; 122:251-6.
12. Bijli KM, Singh BP, Sridhara S, Gaur SN, Arora N. Standardizing Imperata cylindrica-source material for quality allergen preparations. J Immunol Meth 2002; 260:91-6.
13. Lowry OH, Rosenborough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265-73.
14. Troll W, Sherry S. The activation of human plasminogen by streptokinase. J Biol Chem 1955; 213:881-91.
15. Kok P, Astrup T. Differentiation between plasminogen activators by means of epsilon-aminocaproic acid. Thromb Diasthesis Haemorrhagica 1972; 27:77-87.
16. Alkjaersig N, Fletcher AP, Sherry S. Epsilon-aminocaproic acid: an inhibitor of plasminogen activation. J Biol Chem 1959; 234:832-7.
17. Voght W, Schmidt G, Lynen R, Dieminger L. Cleavage of the third complement component (C3) and generation of the spasmogenic peptide C3a, in human serum via the properdin pathway: demonstration of inhibitory as well as enhancing effects of epsilon-aminocaproic acid. J Immunol 1975; 144:671-7.
18. Hoeyveld EM, Lejoly M, Walravens MJF, Stevens EAM. Analysis of the stabilizing effect of epsilon-aminocaproic acid by electrophoretic techniques and immunoblotting. J Allergy Clin Immunol 1989; 83:601-9.
19. Sridhara S, Arora A, Singh BP. Stability of allergen extracts. Ind J Allergy Appl Immunol 1998; 12:14.