Turbidity assay for rapid and efficient identification of high protein digestibility sorghum lines

Cereal Chemistry

Volumn 80, Issue 1, 2003, Pages 40-44

  Aboubacar, Adam ^a   Axtell, John D ^a   Nduulu, Lexington ^a   Hamaker, Bruce R ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINS; TRICHLOROACETIC ACID;

2-MERCAPTOETHANOL; ABSORBANCES; BAND INTENSITY; BICINCHONINIC ACIDS; HIGH-THROUGHPUT; PROTEIN DIGESTIBILITY; SDS-PAGE; TURBID SOLUTIONS; VISUAL OBSERVATIONS; WILD TYPES;

TURBIDITY;

BORIC ACID; MERCAPTOETHANOL; PEPSIN A; TRICHLOROACETIC ACID;

ABSORPTION; ARTICLE; FOOD ANALYSIS; MOLECULAR STABILITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SORGHUM; TURBIDITY;

EID: 0037242762     PISSN: 00090352     EISSN: None     Source Type: Journal    
DOI: 10.1094/CCHEM.2003.80.1.40     Document Type: Article

References (18)

1. Aboubacar, A., Axtell, J. D., Huang, C.-P., and Hamaker, B. R. 2001. A rapid protein digestibility assay for identifying highly digestible sorghum lines. Cereal Chem. 78:160-65.
2. American Association of Cereal Chemists. 2000. Approved Methods of the AACC, 10th ed. Method 46-13. The Association: St. Paul, MN.
3. Bensadoun, A., and Weinstein, D. 1976. Assay of proteins in the presence of interfering materiels. Anal. Biochem. 70:241-250.
4. Craine, E. M., Jones, C. A., and Boundy, J. A. 1957. A rapid turbidimetric method for the determination of zein. Cereal Chem. 34:456-463.
5. Hamaker, B. R., Kirleis, A. W., Butler, L. G., Axtell, J. D., and Mertz, E. T. 1987. Improving the in vitro protein digestibility of sorghum with reducing agents. Proc. Natl. Acad. Sci. 84:626-628.
6. Hamaker, B. R., Mohamed, A. A., Habben, J. E., Huang, C. P., and Larkins, B. A. 1995. Efficient procedure for extracting maize and sorghum kernel proteins reveals higher prolamin contents than the conventional method. Cereal Chem. 72:583-588.
7. MacLean, W. C., de Romana, G. L., Placko, R. P., and Graham, G. G. 1981. Protein quality and digestibility of sorghum in preschool children: Balance studies and plasma free amino acids. J. Nutr. 111:1928-1936.
8. Mertz, E. T., Hassen, M. M., Cairns-Whittern, C., Kirleis, A. W., Tu, L., and Axtell, J. D. 1984. Pepsin digestibility of proteins in sorghum and other major cereals. Proc. Natl. Acad. Sci. 81:1-2.
9. Mossé, J. 1990. Nitrogen to protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content. J. Agric. Food Chem. 38:18-24.
10. Oria, M. P., Hamaker, B. R., and Shull, J. M. 1995a. In vitro protein digestibility of developing and mature sorghum grain in relation to α-, β- and γ-kafirin disulfide crosslinking. J. Cereal Sci. 22:85-93.
11. Oria, M. P., Hamaker, B. R., and Shull, J. M. 1995b. Resistance of sorghum α-, β-, and γ-kafirins to pepsin digestion. J. Agric. Food Chem. 43:2148-2152.
12. Oria, M. P., Hamaker, B. R., Axtell, J. D., and Huang, C. P. 2000. A highly digestible sorghum mutant cultivar exhibits a unique folded structure of endosperm protein bodies. Proc. Natl. Acad. Sci. 97:5065-5070.
13. Paulis, J. W., Wall, J. S., and Kwolek, W. F. 1974. A rapid turbidimetric analysis for zein in corn and its correlation with lysine content. J. Agric. Food Chem. 22:313-317.
14. Pedersen, B., and Eggum, B. O. 1983. Prediction of protein digestibility by an in vitro enzymatic pH-stat procedure. Tierphysiol. Tierernaehr. Futtermittelk De. 49:265-277.
15. Shull, J. M., Watterson, J. J., and Kirleis, A. W. 1991. Proposed nomenclature for the alcohol-soluble proteins (kafirins) of Sorghum bicolor (L. Moench) based on molecular weight, solubility, and structure. J. Agric. Food Chem. 39:249-255.
16. Shull, J. M., Watterson, J. J., and Kirleis, A. W. 1992. Purification and immunocytochemical localization of kafirins in Sorghum bicolor (L. Moench) endosperm. Protoplasma 171:64-74.
17. Weaver, C. A., Hamaker, B. R., and Axtell, J. D. 1998. Discovery of grain sorghum germplasm with high uncooked and cooked in vitro protein digestibilities. Cereal Chem. 75:665-670.
18. Young V. R., Bier, D. M., and Pellett, P. L. 1989. A theoretical basis for increasing current estimates of the amino acid requirements in adult man, with experimental support. Am. J. Clin. Nutr. 50:80-92.