Efficacy of a polyurethane foam/spheroid artificial liver by using human hepatoblastoma cell line (Hep G2)

Cell Transplantation

Volumn 12, Issue 1, 2003, Pages 51-58

  Fukuda, J ^a   Okamura, K ^a   Nakazawa, K ^c   Ijima, H ^a   Yamashita, Y ^b   Shimada, M ^b   Shirabe, K ^b   Tsujita, E ^b   Sugimachi, K ^b   Funatsu, K ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c UNIVERSITY OF KITAKYUSHU   (Japan)

Author keywords

Hep G2; Human primary hepatocytes; Hybrid artificial liver; Polyurethane foam; Porcine primary hepatocytes; Spheroids

Indexed keywords

ALBUMIN; AMMONIA; FIBRINOGEN; POLYURETHAN FOAM;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTIFICIAL LIVER; CELL COUNT; CELL DENSITY; CELL PROLIFERATION; CELL SIZE; CELL STRAIN HEPG2; CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; HUMAN; HUMAN CELL; LIVER CELL; LIVER CELL CULTURE; MONOLAYER CULTURE; MULTICELLULAR SPHEROID; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; PROTEIN SYNTHESIS;

ANIMALIA; SUIDAE;

EID: 0037241895     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/000000003783985151     Document Type: Article

References (33)

1. Aden, D. P.; Fogel, A.; Plotkin, S.; Damjanov, I.; Knowles, B. B. Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 282(5739):615-616; 1979.
2. Berthiaume, F.; Moghe, P. V.; Toner, M.; Yarmush, M. L. Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: Hepatocytes cultured in a sandwich configuration. FASEB J. 10: 1471-1484; 1996.
3. Choi, Y. S.; Lee, D-Y.; Kim, I. Y.; Kang, S.; Ahn, K.; Kim, H. J.; Jeong, Y. H.; Chun, G-T.; Park, J. K.; Kim, I-H. Ammonia removal using hepatoma cells in mammalian cell cultures. Biotechnol. Prog. 16:760-768; 2000.
4. Chua, K. B.; Bellini, W. J.; Rota, P. A.; Harcourt, B. H.; Tamin, A.; Lam, S. K.; Ksiazek, T. G.; Rollin, P. E.; Zaki, S. R.; Shieh, W.; Goldsmith, C. S.; Gubler, D. J.; Roehrig, J. T.; Eaton, B.; Gould, A. R.; Olson, J.; Field, H.; Daniels, P.; Ling, A. E.; Peters, C. J.; Anderson, L. J.; Mahy, B. W. Nipah virus: A recently emergent deadly paramyxovirus. Science 288(5470):1432-1435; 2000.
5. Ellis, A. J.; Hughes, R. D.; Wendon, J. A.; Dunne, J.; Langley, P. G.; Kelly, J. H.; Giaslason, G. T.; Sussman, N. L.; Williams, R. Pilot-controlled trial of the extracorporeal liver assist device in acute liver failure. Hepatology 24:1446-1451; 1996
6. Enosawa, S.; Miyashita, T.; Suzuki, S.; Li, X.; Tsunoda, M.; Amemiya. H.; Yamanaka, M.; Hiramatsu, S.; Tanimura, N.; Omasa. T.; Suga. K.; Matsumura, T. Long-term culture of glutamine synthetase-transfected Hep G2 cells in circulatory flow bioreactor for development of a bioartificial liver. Cell Transplant. 9:711-715; 2000.
7. Enosawa, S.; Suzuki, S.; Kakefuda, T.; Amemiya, H. Examination of 7-ethoxycoumarin deethylation and ammonia removal activities in 31 hepatocyte cell lines. Cell Transplant. 5:S39-S40; 1996.
8. Fukuda, J.; Sakiyama, R.; Nakazawa, K.; Ijima, H.; Yamashita, Y.; Shimada, M.; Shirabe, K.; Tsujita, E.; Sugimachi, K.; Funatsu, K. Mass preparation of primary porcine hepatocytes and the design of a hybrid artificial liver module using spheroid culture for a clinical trial. Int. J. Artif. Organs 24:799-806; 2001.
9. Funatsu, K.; Ijima, H.; Nakazawa, K.; Yamashita, Y.; Shimada, M.; Sugimachi, K. Hybrid artificial liver using hepatocyte organoid culture. Artif. Organs 25(3):194-200; 2001.
10. Gerlach, J. C. Development of a hybrid liver support system: A review. Int. J. Artif. Organs 19(11):645-654; 1996.
11. Gion, T.; Shimada, M.; Shirabe, K.; Nakazawa, K.; Ijima, H.; Matsushita, T.; Funatsu, K.; Sugimachi, K. Evaluation of a hybrid artificial liver using a polyurethane foam packed-bed culture system in dogs. J. Surg. Res. 82:131-136; 1999.
12. Ijima, H.; Matsushita, T.; Nakazawa, K.; Fujii, Y.; Funatsu, K. Hepatocyte spheroids in polyurethane foams: Functional analysis and application for a hybrid artificial liver. Tissue Eng. 4:213-226; 1998.
13. Ijima, H.; Nakazawa, K.; Koyama, S.; Kaneko, M.; Matsushita, T.; Gion, T.; Shirabe, K.; Shimada, M.; Takenaka, K.; Sugimachi, K.; Funatsu, K. Conditions required for a hybrid artificial liver support system using a PUF/hepatocyte-spheroid packed-bed module and its use in dogs with liver failure. Int. J. Artif. Organs 23:446-453; 2000.
14. Ijima, H.; Nakazawa, K.; Koyama, S.; Kaneko, M.; Matsushita, T.; Gion, T.; Shirabe, K.; Shimada, M.; Takenaka, K.; Sugimachi, K.; Funatsu, K. Development of a hybrid artificial liver using a polyurethane foam/hepatocytespheroid packed-bed module. Int. J. Artif. Organs 23:389-397; 2000.
15. Ijima, H.; Nakazawa, K.; Mizumoto, H.; Matsushita, T.; Funatsu, K. Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver. J. Biomater. Sci. Polym. Ed. 9: 765-778; 1998.
16. Khalil, M.; Shariat-Panahi, A.; Tootle, R.; Ryder, T.; McCloskey, P.; Roberts, E.; Hodgson, H.; Selden, C. Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function. J. Hepatol. 297: 68-77; 2001.
17. Koide, N.; Sakaguchi, K.; Koide, Y.; Asano, K.; Kawaguchi, M.; Matsushima, H.; Takenami, T.; Shinji, T.; Mori, M.; Tsuji, T. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp. Cell Res. 186:227-235; 1990.
18. Matsushita, T.; Ijima, H.; Koide, N.; Funatsu, K. High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam. Appl. Microbiol. Biotechnol. 36:324-326; 1991.
19. Nakazawa, K.; Ijima, H.; Fukuda, J.; Sakiyama, R.; Yamashita, Y.; Shimada, M.; Shirabe, K.; Tsujita, E.; Sugimachi, K.; Funatsu, K. Development of a hybrid artificial liver using polyurethane foam/hepatocyte spheroid culture in a preclinical pig experiment. Int. J. Artif. Organs 25:51-60; 2002.
20. Nakazawa, K.; Mizumoto, H.; Kaneko, M.; Ijima, H.; Gion, T.; Shimada, M.; Shirabe, K.; Takenaka, K.; Sugimachi, K.; Funatsu, K. Formation of porcine hepatocyte spherical multicellular aggregates (spheroid) and analysis of drug metabolic functions. Cytotechnology 31:61-68; 1999.
21. Patience, C.; Takeuchi, Y.; Weiss, R. A. Infection of human cells by an endogenous retrovirus of pigs. Nat. Med. 3:282-286; 1997.
22. Patzer, J. F., II; Mazariegos, G. V.; Kramer, D. J.; Lopez, R.; Giraldo, M.; de Vera, M.; McCormick, E.; Grogan, T. A.; Zhu, Y.; Fulmer, M. L.; Amiot, B. P.; Starzl, T. E. Novel bioartificial liver support system (BLSS): Clinical experience. Int. J. Artif. Organs 22:407; 1999.
23. Public Health Service. PHS guideline on infectious Issues in xenotransplantation; Preamble. January 19; 2001.
24. Takahashi, M.; Matue, H.; Matsushita, M.; Nakajima, Y.; Uchino, J. Isolation and culture of human hepatocytes from resected liver tissue as a bioreactor for a hybrid artificial liver. Artif. Organs 17:653-659; 1993.
25. van der Laan, L. J.; Lockey, C.; Griffeth, B. C.; Frasier, F. S.; Wilson, C. A.; Onions, D. E.; Hering, B. J.; Long, Z.; Otto, E.; Torbett, B. E.; Salomon, D. R. Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice. Nature 407:90-94; 2000.
26. Wang, L.; Sun, J.; Li, L.; Mears, D.; Horvat, M.; Sheil, A. G. R. Comparison of porcine hepatocytes with human hepatoma (C3A) cells for use in a bioartificial liver support system. Cell Transplant. 7(5):459-468; 1998.
27. Watanabe, F. D.; Mullon, C. J-P.; Hewitt, W. R.; Arkadopoulos, N.; Kahaku, E.; Eguchi, S.; Khalili, T.; Arnaout, W.; Shackleton, C. R.; Rozga, J.; Solomon, B.; Demetriou, A. A. Clinical experience with a bioartificial liver in the treatment of severe liver failure: A phase I clinical trial. Ann. Surg. 225:484-494; 1997.
28. Weiss, R. A. Retroviral zoonoses. Nat. Med. 4(4):391-392; 1998.
29. Wezel, van, A. L. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature 216: 64-65; 1967.
30. Yamamoto, H.; Fujimoto, J.; Okamoto, E.; Furuyama, E.; Tamaoki, T.; Hashimoto-Tamaoki, T. Suppression of growth of hepatocellular carcinoma by sodium butyrate in vitro and in vivo. Int. J. Cancer 76:897-902; 1998.
31. Yamashita, Y.; Shimada, M.; Tsujita, E.; Tanaka, S.; Ijima, H.; Nakazawa, K.; Sakiyama, R.; Fukuda, J.; Ueda, T.; Funatsu, K.; Sugimachi, K. Polyurethane foam/spheroid culture system using human hepatoblastoma cell line (Hep G2) as a possible new hybrid artificial liver. Cell Transplant. 10:717-722; 2001.
32. Yoon, J-H.; Lee, H. V-S.; Lee, J. S.; Park, J. B.; Kim, C. Y. Development of a non-transformed human liver cell line with defferentiated-hepatocyte and urea-synthetic functions: Applicable for bioartificial liver. Int. J. Artif. Organs 22(11):769-777; 1999.
33. Zvibel, I.; Fiorino, A. S.; Brill, S.; Reid, L. M. Phenotypic characterization of rat hepatoma cell lines and lineage- specific regulation of gene expression by differentiation agents. Differentiation 63:215-223; 1998.