Development of real-time PCR for the rapid detection of episomal Banana streak virus (BSV)

Plant Disease

Volumn 87, Issue 1, 2003, Pages 33-38

  Delanoy, M ^a   Salmon, M ^a   Kummert, J ^a   Frison, E ^b   Lepoivre, P ^c  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b Parc Scientifique   (France)

^c NONE

Author keywords

5 nuclease assay; Badnavirus; Long amplicon; Minor groove binder (MGB); Musa

Indexed keywords

BINDERS; BIOASSAY; DNA; GENES; PLANTS (BOTANY);

EPISOMAL BANANA STREAK VIRUSES (BSV);

VIRUSES;

BADNAVIRUS; BANANA STREAK VIRUS; MUSA; PLANTAGO;

EID: 0037228786     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS.2003.87.1.33     Document Type: Article

References (26)

1. Bustin, S. A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:169-193.
2. Dahal, G., Ortiz, R., Tenkouano, A., d'A Hugues, J., Thottappilly, G., Vuylsteke, D., and Lockhart, B. 2000. Relationship between natural occurrence of Banana streak badnavirus and symptom expression, relative concentration of viral antigen, and yield characteristics of some micropropagated Musa spp. Plant Pathol. 49:68-79.
3. Daniells, J. W., Geering, A. D. W., Bryde, N. J., and Thomas, J. E. 2001. The effect of Banana streak virus on the growth and yield of dessert bananas in tropical Australia. Ann. Appl. Biol. 139:51-60.
4. Delanoy, M., Jijakli, H., and Lepoivre, P. 2001. Detection of Banana streak virus (BSV) by IC-PCR-ELOSA. (Abstr.) Phytopathology 91:S22.
5. Diekmann, D. R., and Putter, C. A. J. 1996. Musa FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm, 2nd ed. Rome.
6. Geering, A., McMichael, L., Dietzgen, R., and Thomas, J. 2000. Genetic diversity among Banana streak virus isolates from Australia. Phytopathology 90:921-927.
7. Geering, A., Olszewski, N. E., Dahal, G., Thomas, J., and Lockhart, B. 2001. Analysis of the distribution and structure of integrated Banana streak virus DNA in a range of Musa cultivars. Mol. Plant. Pathol. 2:207-213.
8. Harper, G., Dahal, G., Thottappilly, G., and Hull, R. 1999. Detection of episomal Banana streak badnavirus by IC-PCR. J. Virol. Methods 79:1-8.
9. Harper, G., and Hull, R. 1998. Cloning and sequence analysis of Banana streak virus DNA. Virus Genes 17:1-9.
10. Harper, G., Osuji, J., Heslop-Harrison, J., and Hull, R. 1999. Integration of Banana streak badnavirus into the Musa genome: molecular and cytogenetic evidence. Virology 255:207-213.
11. Helliot, B., Panis, B., Poumay, Y., Swennen, R., Lepoivre, P., and Frison, E. 2002. Cryopreservation for the elimination of Cucumber mosaic and Banana streak viruses from banana (Musa spp.). Plant Cell Rep. 20:1117-1122.
12. Holland, P. M., Abramson, R. D., Watson, R., and Gelfand, D. H. 1991. Detection of specific polymerase chain reaction products by utilising 5′ → 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88:7276-7280.
13. Kutyavin, I. V., Afonina, I. A., Mills, A., Gorn, V. V., Lukhtanov, E. A., Belousov, E. S., Singer, M. J., Walburger, D. K., Lokhov, S. G., Gall, A. A., Dempcy, R., Reed, M. W., Meyer, R. B., and Hedgpeth, J. 2000. 3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperature. Nucleic Acids Res. 28:655-661.
14. Kutyavin, I. V., Lukhtanov, E. A., Gamper, H. B., and Meyer, R. B. 1997. Oligonucleotides with conjugated dihydropyrroloindole tripeptides : base composition and backbone effects on hybridization. Nucleic Acids Res. 25:3718-3723.
15. Livak, K. J., Flood, S. J. A., Marmaro, J., Giusti, W., and Deetz, K. 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Applications 4, 357-362.
16. Lockhart, B. 1990. Evidence for a double-stranded circular genome in a second group of plant viruses. Phytopathology 80:127-131.
17. Lockhart, B., and Olszewski, N. 1993. Serological and genomic heterogeneity of Banana streak badnavirus: implications for virus detection in Musa germplasm. Pages 105-113 in: Breeding Banana and Plantain for Resistance to Diseases and Pests. J. Ganry, ed. Proc. Int. Symp. Genet. Improv. Bananas Resist. Dis. Pests, CIRAD-FHLOR and INIBAP, Montpellier, France.
18. Mackay, I. M., Arden, K. E., and Nitsche, A. 2002. Real-time PCR in virology. Nucleic Acids Res. 30:1292-1305.
19. Ndowora, T. 1998. Development of an enzyme immunoassay to detect serologically diverse isolates of Banana streak virus and characterization of viral sequences integrated into the Musa genome. Ph.D. thesis. University of Minnesota, St. Paul.
20. Ndowora, T., Dahal, G., LaFleur, D., Harper, G., Hull, R., Olszewski, N., and Lockhart, B. 1999. Evidence that badnavirus infection in Musa can originate from integrated pararetroviral sequences. Virology 255:214-220.
21. Ortiz, R. 1996. The potential of AMMI analysis for field assessment of Musa genotypes to virus infection. Hortscience 31:829-832.
22. Salmon, M. 2002. La PCR en temps réel. Biofutur 219, Technoscope 139.
23. Salmon, M., Vendrame, M., Kummert, J., and Lepoivre, P. 2002. Detection of Apple chlorotic leaf spot virus using a 5′ nuclease assay with a fluorescent 3′ minor groove binder-DNA probe. J. Virol. Methods 104:99-106.
24. Salmon, M., Vendrame, M., Kummert, J., and Lepoivre, P. Rapid and homogenous detection of Apple stem pitting virus by RT-PCR and a fluorogenic 3′ minor groove binder-DNA probe. Eur. J. Plant Pathol. In press.
25. Spiegel, S., Scott, S. W., Bowman-Vance, V., Tam, Y., Galiakparov, N. N., and Rosner, A. 1996. Improved detection of Prunus necrotic ringspot virus by the polymerase chain reaction. Eur. J. Plant Pathol. 102:681-685.
26. Thottappilly, G., Dahal, G., and Lockhart, B. 1998. Studies on a Nigerian isolate of Banana streak badnavirus: I. Purification and enzyme-linked immunosorbent assay. Ann. Appl. Biol. 132:253-261.