Enterotoxigenicity and cytotoxicity of Bacillus thuringiensis strains and development of a process for Cry1Ac production

Journal of Agricultural and Food Chemistry

Volumn 51, Issue 1, 2003, Pages 100-105

  Yang, Chi Yea ^c   Pang, Jen Chieh ^a   Kao, Suey Sheng ^b   Tsen, Hau Yang ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b TAIWAN AGRICULTURAL CHEMICALS AND TOXIC SUBSTANCES RESEARCH INSTITUTE   (Taiwan)

^c VAN NUNG INSTITUTE OF TECHNOLOGY   (Taiwan)

Author keywords

Bacillus thuringiensis; BCET RPLA kit; Cry1Ac; Enterotoxin; Food safety

Indexed keywords

AMYLASE; BACILLUS THURINGIENSIS CRYSTAL PROTEIN; BACILLUS THURINGIENSIS TOXIN; BACTERIAL PROTEIN; DNA FRAGMENT; ENDOTOXIN; ENTEROTOXIN; INSECTICIDE; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BIOLOGICAL PEST CONTROL; BIOSYNTHESIS; CELL DEATH; CHO CELL; CONTROLLED STUDY; CYTOTOXICITY; DNA VECTOR; DRUG EFFECT; EXPRESSION VECTOR; FOOD SAFETY; GASTROINTESTINAL TOXICITY; GENE AMPLIFICATION; GENE EXPRESSION; GENETICS; HAMSTER; INSECTICIDAL ACTIVITY; METABOLISM; MOLECULAR CLONING; NONHUMAN; PATHOGENICITY; PLASMID; PROMOTER REGION;

ALPHA-AMYLASE; ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL DEATH; CHO CELLS; CRICETINAE; ENDOTOXINS; ENTEROTOXINS; GENE EXPRESSION; GENETIC VECTORS; HEMOLYSIN PROTEINS; INSECTICIDES; PEST CONTROL, BIOLOGICAL; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS;

ANIMALIA; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); CRICETINAE; CRICETULUS GRISEUS; TRICHOPLUSIA NI; VECTORS;

EID: 0037228386     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf025863l     Document Type: Article

References (30)

1. Schnepf, E.; Crickmore, N.; Van, R. J.; Lereclus, D.; Baum, J.; Feitelson, J.; Zeigler, D. R.; Dean, D. H. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 1998, 62, 775-806.
2. Notermans, S.; Batt, C. A. A risk assessment approach for foodborne Bacillus cereus and its toxins. J. Appl. Microbiol. Symp. Suppl. 1998, 84, 51s-61s.
3. Beecher, D. J.; Wong, A. C. Tripartite hemolysin BL from Bacillus cereus. Hemolytic analysis of component interaction and model for its characteristic paradoxical zone phenomenon. J. Biol. Chem. 1997, 272, 233-239.
4. Beecher, D. J.; Schoeni, J. L.; Wong, A. C. Enterotoxin activity of hemolysin BL from Bacillus cereus. Infect. Immun. 1995, 63, 4423-4428.
5. Lund, T.; Granum, P. E. Characterization of a non-haemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak. FEMS Microbiol. Lett. 1996, 141, 151-156.
6. Ash, C.; Collins, M. D. Comparative analysis of 23S ribosomal RNA gene sequences of Bacillus anthracis and emetic Bacillus cereus determined by PCR-direct sequencing. FEMS Microbiol. Lett. 1992, 94, 75-80.
7. Barjac, H.; Frachon, E. Classification of Bacillus thuringiensis strains. Entomophaga 1990, 35, 233-240.
8. Carlson, C. R.; Johansen, T.; Kolsto, A. B. The chromosome map of Bacillus thuringiensis subsp. canadensis HD244 is highly similar to that of Bacillus cereus type strain ATCC 14579. FEMS Microbiol. Lett. 1996, 141, 163-167.
9. Perani, M.; Bishop, A. H.; Vaid, A. Prevalence of β-exotoxin, diarrhoeal toxin and specific δ-endotoxin in natural isolates of Bacillus thuringiensis. FEMS Microbiol. Lett. 1998, 160, 55-60.
10. Damgaard, P. H. Diarrhoeal enterotoxin production by strains of Bacillus thuringiensis isolated from commercial Bacillus thuringiensis-based insecticides. FEMS Immunol. Med. Microbiol. 1995, 12, 245-250.
11. Hansen, B. M.; Hendriksen, N. B. Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis. Appl. Environ. Microbiol. 2001, 67, 185-189.
12. Yamamoto, T. Identification of entomocidal toxins of Bacillus thuringiensis by high-performance liquid chromatography. In Analytical Chemistry of Bacillus Thuringiensis; Hickle, L. A., Fitch, W. L., Eds.; American Chemical Society: Washington, DC, 1990; pp 46-60.
13. Buchanan, R. L.; Schultz, F. J. Evaluation of the Oxoid BCETRPLA kit for the detection of Bacillus cereus diarrhoeal enterotoxin and compared to cell culture cytotoxicity. J. Food Prot. 1992, 55, 440-443.
14. Chak, K. F.; Tseng, M. Y.; Yamamoto, T. Expression of the crystal protein gene under the control of the α-amylase promoter in Bacillus thuringiensis strains. Appl. Environ. Microbiol. 1994, 60, 2304-2310.
15. Gleave, A. P.; Hedges, R. J.; Broadwell, A. H.; Wigley, P. J. Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae). N.Z. J. Crop Hortic. Sci. 1992, 20, 27-36.
16. Kado, C. I.; Liu, S. T. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 1981, 145, 1365-1373.
17. Sambrook, J.; Fritch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd Ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
18. Lopez-Meza, J. E.; Ibarra, J. E. Characterization of a novel strain of Bacillus thuringiensis. Appl. Environ. Microbiol. 1996, 62, 1306-1310.
19. Graham, D. Y.; Conlong, D. E. Improved laboratory rearing of Eldana saccharina (Lepidoptera: Pyralidae) and its indigenous parasitoid Goniozus natalensis (Hyenoptera: Bethylidae) Proc. S. Afr. Sugar Technol. Assoc. Annu. Congr. 1988, 62, 116-119.
20. Finney, D. J. Probit Analysis, 3rd Ed.; Cambridge University Press: Cambridge, U.K., 1977.
21. Gaviria, R. A. M.; Granum, P. E.; Priest, F. G. Common occurrence of enterotoxin genes and enterotoxicity in Bacillus thuringiensis. FEMS Microbiol. Lett. 2000, 190, 151-155.
22. Hsieh, Y. M.; Sheu, S. J.; Chen, Y. L.; Tsen, H. Y. Enterotoxigenic profiles and polymerase chain reaction detection of Bacillus cereus group cells and B. cereus strains from foods and foodborne outbreaks. J. Appl. Microbiol. 1999, 87, 481-490.
23. Lee, H. K.; Gill, S. S. Molecular cloning and characterization of a novel mosquitocidal protein gene from Bacillus thuringiensis subsp. fukuokaensis. Appl. Environ. Microbiol. 1997, 63, 4664-4670.
24. Shao, Z.; Liu, Z.; Yu, Z. Effects of the 20-kilodalton helper protein on Cry1Ac production and spore formation in Bacillus thuringiensis. Appl. Environ. Microbiol. 2001, 67, 5362-5369.
25. Wu, D.; Federici, B. A. A 20-kilodalton protein preserves cell viability and promotes CytA crystal formation during sporulation in Bacillus thuringiensis. J. Bacteriol. 1993, 175, 5276-5280.
26. Chang, C.; Yu, Y. M.; Dai, S. M.; Law, S. K.; Gill, S. S. Highlevel cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes. Appl. Environ. Microbiol. 1993, 59, 815-821.
27. Andrews, R. E. J.; Bibilos, M. M.; Bulla, L. A. Protease activation of entomocidal protoxin of Bacillus thuringiensis subsp. kurstaki. Appl. Environ. Microbiol. 1985, 50, 732-742.
28. Nicholson, W. L.; Park, Y. K.; Henkin, T. M.; Won, M.; Weickert, M. J.; Gaskell, J. A.; Chambliss, G. H. Catabolite repression resistant mutation of the Bacillus subtilis α-amylase promoter affect transcription levels and are in an operator-like sequence. J. Mol. Biol. 1987, 198, 609-618.
29. Mohan, M.; Gujar, G. T. Susceptibility pattern and development of resistance in the diamondback moth, Plutella xylostella L, to Bacillus thuringiensis Berl var kurstaki in India. Pest Manage. Sci. 2000, 56, 189-194.
30. Damgaard, P. H.; Granum, P. E.; Bresciani, J.; Torregrossa, M. V.; Eilenberg, J.; Valentino, L. Characterization of Bacillus thuringiensis isolated from infections in burn wounds. FEMS Immunol. Med. Microbiol. 1997, 18, 47-53.