A live, attenuated dengue virus type 1 vaccine candidate with a 30-nucleotide deletion in the 3′ untranslated region is highly attenuated and immunogenic in monkeys

Journal of Virology

Volumn 77, Issue 2, 2003, Pages 1653-1657

  Whitehead, Stephen S ^a   Falgout, Barry ^b   Hanley, Kathryn A ^a   Blaney Jr , Joseph E ^a   Markoff, Lewis ^b   Murphy, Brian R ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DENGUE VACCINE; LIVE VACCINE;

3' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; DELETION MUTANT; DENGUE VIRUS; GENE DELETION; IMMUNOGENICITY; INOCULATION; MOSQUITO; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHESUS MONKEY; SEROTYPE; TOXORHYNCHITES SPLENDENS; VIRUS ATTENUATION; VIRUS REPLICATION;

3' UNTRANSLATED REGIONS; ANIMALS; ANTIBODIES, VIRAL; BASE SEQUENCE; DENGUE VIRUS; MACACA MULATTA; MICE; MICE, SCID; NEUTRALIZATION TESTS; NUCLEIC ACID CONFORMATION; RNA, VIRAL; SEQUENCE DELETION; VIRAL VACCINES;

EID: 0037223896     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.2.1653-1657.2003     Document Type: Article

References (22)

1. An, J., J. Kimura-Kuroda, Y. Hirabayashi, and K. Yasui. 1999. Development of a novel mouse model for dengue virus infection. Virology 263:70-77.
2. Blaney, J. E., Jr., D. H. Johnson, G. G. Manipon, C. Y. Firestone, C. T. Hanson, B. R. Murphy, and S. S. Whitehead. 2002. Genetic basis of attenuation of dengue virus type 4 small plaque mutants with restricted replication in suckling mice and in SCID mice transplanted with human liver cells. Virology 300:125-139.
3. Brinton, M. A., A. V. Fernandez, and J. H. Dispoto. 1986. The 3′-nucleotides of flavivirus genomic RNA form a conserved secondary structure. Virology 153:113-121.
4. Butrapet, S., C. Y.-H. Huang, D. J. Pierro, N. Bhamarapravati, D. J. Gubler, and R. M. Kinney. 2000. Attenuation markers of a candidate dengue type 2 vaccine virus, strain 16681 (PDK-53), are defined by mutations in the 5′ noncoding region and nonstructural proteins 1 and 3. J. Virol. 74:3011-3019.
5. Durbin, A. P., R. A. Karron, W. Sun, D. W. Vaughn, M. J. Reynolds, J. R. Perreault, B. Thumar, R. Men, C. J. Lai, W. R. Elkins, R. M. Chanock, B. R. Murphy, and S. S. Whitehead. 2001. Attenuation and immunogenicity in humans of a live dengue virus type-4 vaccine candidate with a 30 nucleotide deletion in its 3′-untranslated region. Am. J. Trop. Med. Hyg. 65:405-413.
6. Gubler, D. J., and M. Meltzer. 1999. Impact of dengue/dengue hemorrhagic fever on the developing world. Adv. Virus Res. 53:35-70.
7. Guillot, S., V. Caro, N. Cuervo, E. Korotkova, M. Combiescu, A. Persu, A. Aubert-Combiescu, F. Delpeyroux, and R. Crainic. 2000. Natural genetic exchanges between vaccine and wild poliovirus strains in humans. J. Virol. 74:8434-8443.
8. Guirakhoo, F., J. Arroyo, K. V. Pugachev, C. Miller, Z. X. Zhang, R. Weltzin, K. Georgakopoulos, J. Catalan, S. Ocran, K. Soike, M. Ratterree, and T. P. Monath. 2001. Construction, safety, and immunogenicity in nonhuman primates of a chimeric yellow fever-dengue virus tetravalent vaccine. J. Virol. 75:7290-7304.
9. Hahn, C. S., Y. S. Hahn, C. M. Rice, E. Lee, L. Dalgarno, E. G. Strauss, and J. H. Strauss. 1987. Conserved elements in the 3′ untranslated region of flavivirus RNAs and potential cyclization sequences. J. Mol. Biol. 198:33-41.
10. Huang, C. Y.-H., S. Butrapet, D. J. Pierro, G. J. Chang, A. R. Hunt, N. Bhamarapravati, D. J. Gubler, and R. M. Kinney. 2000. Chimeric dengue type 2 (vaccine strain PDK-53)/dengue type 1 virus as a potential candidate dengue type 1 virus vaccine. J. Virol. 74:3020-3028.
11. Markoff, L., X. Pang, H.-S. Houng, B. Falgout, R. Olsen, E. Jones, and S. Polo. 2002. Derivation and characterization of a dengue type 1 host range-restricted mutant virus that is attenuated and highly immunogenic in monkeys. J. Virol. 76:3318-3328.
12. Men, R., M. Bray, D. Clark, R. M. Chanock, and C.-J. Lai. 1996. Dengue type 4 virus mutants containing deletions in the 3′ noncoding region of the RNA genome: Analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys. J. Virol. 70:3930-3937.
13. Polo, S., G. Ketner, R. Levis, and B. Falgout. 1997. Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast. J. Virol. 71:5366-5374.
14. Proutski, V., E. A. Gould, and E. C. Holmes. 1997. Secondary structure of the 3′ untranslated region of flaviviruses: Similarities and differences. Nucleic Acids Res. 25:1194-1202.
15. Puri, B., W. M. Nelson, E. A. Henchal, C. H. Hoke, K. H. Eckels, D. R. Dubois, K. R. Porter, and C. G. Hayes. 1997. Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells. J. Gen. Virol. 78:2287-2291.
16. Puri, B., S. Polo, C. G. Hayes, and B. Falgout. 2000. Construction of a full length infectious clone for dengue-1 virus Western Pacific 74 strain. Virus Genes 20:57-63.
17. Rauscher, S., C. Flamm, C. W. Mandl, F. X. Heinz, and P. F. Stadler. 1997. Secondary structure of the 3′-noncoding region of flavivirus genomes: Comparative analysis of base pairing probabilities. RNA 3:779-791.
18. Rosen, L., L. E. Roseboom, D. J. Gubler, J. C. Lien, and B. N. Chaniotis. 1985. Comparative susceptibility of mosquito species and strains to oral and parenteral infection with dengue and Japanese encephalitis viruses. Am. J. Trop. Med. Hyg. 34:603-615.
19. Rosen, L., and D. A. Shroyer. 1985. Comparative susceptibility of five species of Toxorhynchites mosquitoes to parenteral infection with dengue and other flaviviruses. Am. J. Trop. Med. Hyg. 34:805-809.
20. Troyer, J. M., K. A. Hanley, S. S. Whitehead, D. Strickman, R. A. Karron, A. P. Durbin, and B. R. Murphy. 2001. A live attenuated recombinant dengue-4 virus vaccine candidate with restricted capacity for dissemination in mosquitoes and lack of transmission from vaccinees to mosquitoes. Am. J. Trop. Med. Hyg. 65:414-419.
21. Worobey, M., A. Rambaut, and E. C. Holmes. 1999. Widespread intraserotype recombination in natural populations of dengue virus. Proc. Natl. Acad. Sci. USA 96:7352-7357.
22. Zeng, L., B. Falgout, and L. Markoff. 1998. Identification of specific nucleotide sequences within the conserved 3′-SL in the dengue type 2 virus genome required for replication. J. Virol. 72:7510-7522.