Detection of thymidylate synthase modulators by a novel screening assay

Molecular Pharmacology

Volumn 63, Issue 1, 2003, Pages 167-173

  Wu, Qi ^a   Dolnick, Bruce J ^a  

^a ROSWELL PARK CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

8 MERCAPTOGUANOSINE; AZACITIDINE; DACTINOMYCIN; FLOXURIDINE; FLUOROURACIL; FLUOROURIDINE; LUCIFERASE; METESIND GLUCURONATE; METHOTREXATE; NOLATREXED; PYRIMIDINE DERIVATIVE; RALTITREXED; THYMIDYLATE SYNTHASE; THYMIDYLATE SYNTHASE INHIBITOR; TRIFLURIDINE;

ARTICLE; BINDING AFFINITY; CANCER CELL; COLON CANCER; CONTROLLED STUDY; DRUG EFFECT; DRUG IDENTIFICATION; ENZYME ACTIVITY; ENZYME ASSAY; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN RNA BINDING; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; VALIDATION PROCESS; WESTERN BLOTTING;

ANTIMETABOLITES, ANTINEOPLASTIC; DNA-BINDING PROTEINS; EARLY GROWTH RESPONSE PROTEIN 1; ENZYME INDUCTION; FLOXURIDINE; FLUOROURACIL; HUMANS; IMMEDIATE-EARLY PROTEINS; LUCIFERASES; PROMOTER REGIONS (GENETICS); THYMIDYLATE SYNTHASE; TRANSCRIPTION FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED; UP-REGULATION;

EID: 0037220642     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.63.1.167     Document Type: Article

References (25)

1. Allegra CJ, Chabner BA, Drake JC, Lutz R, Rodbard D, and Jolivet J (1985) Enhanced inhibition of thymidylate synthase by methotrexate polyglutamates. J Biol Chem 260:9720-9726.
2. Almasan A, Yin Y, Kelly RE, Lee EY, Bradley A, Li W, Bertino JR, and Wahl GM (1995) Deficiency of retinoblastoma protein leads to inappropriate S-phase entry, activation of E2F-responsive genes and apoptosis. Proc Natl Acad Sci USA 92:5436-5440.
3. Black AR and Dolnick BJ (1996) Expression of rTS correlates with altered growth regulation of thymidylate synthase. Cancer Res. 56:700-705.
4. Chu E, Cogliati T, Copur SM, Borre A, Voeller DM, Allegra CJ, and Segal S (1996) Identification of in vivo target RNA sequences bound by thymidylate synthase. Nucleic Acids Res 24:3222-3228.
5. Chu E, Takechi T, Jones KL, Voeller DM, Copur SM, Maley GF, Maley F, Segal S, and Allegra CJ (1995) Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro. Mol Cell Biol 15:179-185.
6. Chu E, Voeller DM, Casey JL, Drake JC, Chabner BA, Elwood PC, Zinn S, and Allegra CJ (1991) Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase. Proc Natl Acad Sci USA 88:8977-8981.
7. Chu E, Voeller DM, Jones KL, Takechi T, Maley GF, Maley F, Segal S, and Allegra CJ (1994a) Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells. Mol Cell Biol 14:207-213.
8. Chu E, Voeller DM, Koeller DM, Drake JC, Takimoto CH, Maley GF, Maley F, and Allegra CJ (1993) Identification of an RNA binding site for human thymidylate synthase. Proc Natl Acad Sci USA 90:517-521.
9. Chu E, Voeller DM, Morrison PF, Jones KL, Takechi T, Maley GF, Maley F, and Allegra CJ (1994b) The effect of reducing agents on binding of thymidylate synthase protein to thymidylate synthase messenger RNA. J Biol Chem 269:20289-20293.
10. Chu E, Zinn S, Boarman D, and Allegra CJ (1990) The interaction of gamma interferon and 5-fluorouracil in the H630 human colon carcinoma cell line. Cancer Res 50:5834-5840.
11. Dolnick BJ and Black AR (1996) Alternate splicing of the rTS gene product and its overexpression in a 5-fluorouracil-resistant cell line. Cancer Res 56:3207-3210.
12. Dolnick BJ, Zhang ZG, Hines JD, and Rustum YM (1992) Quantitation of dihydrofolate reductase and thymidylate synthase mRNAs in vivo and in vitro by polymerase chain reaction. Oncol Res 4:65-72.
13. Drake JC, Allegra CJ, and Johnston PG (1993) Immunological quantitation of thymidylate synthase-FdUMP-5, 10-methylenetetrahydrofolate ternary complex with the monoclonal antibody TS 106. Anticancer Drugs 4:431-435.
14. Friedkin M and Kornberg A (1957) in Chem Basis of Heredity (McElroy WD and Glass B eds) pp 609-614, John Hopkins University Press, Baltimore.
15. Hardy LW, Finer-Moore JS, Montfort WR, Jones MO, Santi DV, and Stroud RM (1987) Atomic structure of thymidylate synthase: Target for rational drug design. Science (Wash DC) 235:448-455.
16. Jackman AL and Calvert AH (1995) Folate-based thymidylate synthase inhibitors as anticancer drugs. Ann Oncol 6:871-881.
17. Kitchens ME, Forsthoefel AM, Barbour KW, Spencer HT, and Berger FG (1999) Mechanisms of acquired resistance to thymidylate synthase inhibitors: The role of enzyme stability. Mol Pharmacol 56:1063-1070.
18. Lin X, Parsels LA, Voeller DM, Allegra CJ, Maley GF, Maley F, and Chu E (2000) Characterization of a cis-acting inhibitory element in the protein coding region of thymidylate synthase mRNA. Nucleic Acids Res 28:1381-1389.
19. Park JG, Oie HK, Sugarbaker PH, henslee JG, Chen TR, Johnson BE and Gazdar A (1987) Characteristics of cell lines established from human colorectal carcinoma. Cancer Res 47:6710-6718.
20. Poirier F, Lawrence D, Vigier P, and Jullien P (1982) A ts T mutant of Schmidt Ruppin strain of Rous sarcoma virus restricted at 39.5°C for the morphological transformation and the tumorigenicity of chicken embryo fibroblasts. Int J Cancer 29:69-76.
21. Santi DV and Danenberg PV (1984) in Folates and Pteridines (Blakley RL and Benkovic SJ eds), vol. 1, pp 345-398, Wiley, New York.
22. Swain SM, Lippman ME, Egan EF, Drake JC, Steinberg SM, and Allegra CJ (1989) 5-Fluorouracil and high-dose leucovorin in previously treated patients with metastatic breast cancer. J Clin Oncol. 7:890-899.
23. Toku S, Nabeshima Y, and Ogata K (1983) Binding of actinomycin D to mRNA in vivo and in vitro. J Biochem (Tokyo) 93:361-366.
24. Voeller DM, Changchien LM, Maley GF, Maley F, Takechi T, Turner RE, Montford WR, Allegra CJ, and Chu E (1995) Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA. Nucleic Acids Res 23:869-875.
25. Welsh SJ, Titley J, Brunton L, Valenti M, Monaghan P, Jackman AL, and Aherne GW (2000) Comparison of thymidylate synthase (TS) protein up-regulation after exposure to TS inhibitors in normal and tumor cell lines and tissues. Clin Cancer Res 6:2538-2546.