The increased bactericidal activity of a fatty acid-modified synthetic antimicrobial peptide of human cathepsin G correlates with its enhanced capacity to interact with model membranes

International Journal of Antimicrobial Agents

Volumn 21, Issue 1, 2003, Pages 13-19

  Mak, Pawel ^a   Pohl, Jan ^b   Dubin, Adam ^a   Reed, Matthew S ^b   Bowers, Samera E ^b   Fallon, Michael T ^b,c   Shafer, William M ^b,c  

^a JAGIELLONIAN UNIVERSITY   (Poland)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c ATLANTA VA MEDICAL CENTER   (United States)

Author keywords

Acylation; Antibacterial; Peptides

Indexed keywords

ANTIINFECTIVE AGENT; ARGINYLPROLYLGLYCYLTHREONYLLEUCYLCYSTEINYLTHREONYLVALYLALANYLGLYCYLTRYPTOPH YLGLYCYLARGINYLVALYLSERYLMETHIONYLARGINYLARGINYLGLYCYLTHREONINE; CATHEPSIN G; FATTY ACID; FLUORESCENT DYE; LIPOSOME; PEPTIDE DERIVATIVE; TRIFLUOROETHANOL; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; ARTIFICIAL MEMBRANE; BACTERICIDAL ACTIVITY; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CIRCULAR DICHROISM; CONTROLLED STUDY; CORRELATION ANALYSIS; DRUG POTENCY; DRUG SYNTHESIS; FEMALE; MOUSE; NEISSERIA GONORRHOEAE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; BACTERIA; CELL MEMBRANE; FATTY ACIDS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0037218673     PISSN: 09248579     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0924-8579(02)00245-5     Document Type: Article

References (27)

1. Hancock R.E.W. Cationic peptides: effectors in innate immunity and novel antimicrobials. Lancet Infect. Dis. 1:2001;156-164.
2. Boman H.G. Antibacterial peptides: key components needed in immunity. Cell. 65:1991;205-207.
3. Mak P., Szewczyk A., Mickowska B., Kiciñska A., Dubin A. Effect of antimicrobial apomyoglobin 56-131 peptide on liposomes and planar lipid bilayer membrane. Int. J. Antimicrob. Agents. 17:2001;137-142.
4. Kelley K.J. Using host defenses to fight infectious diseases. Nat. Biotechnol. 14:1996;587-590.
5. Stone A.B., Hitchcock P.J. Vaginal microbicides for preventing the sexual transmission of HIV. AIDS. Suppl. 1:1994;S285-S293.
6. Odeberg H., Olsson I. Mechanisms for the microbicidal activity of cationic proteins of human granulocytes. Infect. Immun. 14:1975;1269-1275.
7. Odeberg H., Olsson I. Antibacterial activity of cationic proteins from human granulocytes. J. Clin. Invest. 56:1976;1118-1124.
8. Shafer W.M., Onunka V.C., Martin L.E. Antigonococcal activity of human neutrophil cathepsin G. Infect. Immun. 54:1986;184-188.
9. Shafer W.M., Onunka V.C. Mechanisms of staphylococcal resistance to non-oxidative antimicrobial action of human neutrophils: importance of pH and ionic strength in determining the bactericidal activity of cathepsin G. J. Gen. Microbiol. 135:1989;825-830.
10. Shafer W.M., Pohl J., Onunka V.C., Bangalore N., Travis J. Human lysosomal cathepsin G and granzyme B share a functionally conserved broad spectrum antibacterial peptide. J. Biol. Chem. 266:1991;112-116.
11. Shafer W.M., Shepherd M.E., Boltin B.C., Wells R.L., Pohl J. Synthetic peptides of human lysosomal cathepsin G having potent antipseudomonal activity. Infect. Immun. 61:1993;1900-1908.
12. Miyasaki K.T., Bodeau A.L. In vitro killing of oral Capnocytophaga by granule fractions of human neutrophils is associated with cathepsin G activity. J. Clin. Invest. 87:1991;1585-1593.
13. Shafer W.M., Hubalek F., Huang M., Pohl J. The bactericidal activity of a synthetic peptide (CG 117-136) of human lysosomal cathepsin G is dependent on arginine content. Infect. Immun. 64:1996;4842-4845.
14. + flux. Nature. 416:2002;291-297.
15. 2: a janus-faced proteinase with two opposite specificities. EMBO J. 15:1996;5481-5491.
16. Lehrer R.I., Burton A., Daher K., Harwig S.S.L., Ganz T., Selsted M.E. Interaction of human defensins with Escherichia coli: mechanism of bactericidal activity. J. Clin. Invest. 84:1989;553-561.
17. Westerhoff H.V., Juretic D., Hendler R.W., Zasloff M. Magainins and the disruption of membrane-linked free-energy transduction. Proc. Natl. Acad. Sci. USA. 86:1989;6597-6601.
18. Ofek I., Cohen S., Rahmani R., Kabha K., et al. Antibacterial synergism of polymyxin B nonapeptide and hydrophobic antibiotics in experimental gram-negative infections in mice. Antimicrob. Agents Chemother. 38:1994;374-377.
19. Chicharro C., Granata C., Lozano R., Andreu D., Rivas L. N-terminal fatty acid substitution increases the leishmanicidal activity of CA(1-7)M(2-9), a cecropin-melittin hybrid peptide. Antimicrob. Agents Chemother. 45:2001;2441-2449.
20. Shafer W.M., Katzif S., Bowers S., et al. Tailoring an antibacterial peptide of human lysosomal cathepsin G to enhance its broad-spectrum action against antibiotic-resistant bacterial pathogens. Curr. Pharmaceut. Des. 8:2002;695-702.
21. Pasenkiewicz-Gierula M., Murzyn K., Rog T., Czaplewski C. Molecular dynamics simulation studies of lipid bilayer systems. Acta Biochim. Pol. 47:2000;601-611.
22. Shafer W.M., Qu X.-D., Waring A.J., Lehrer R.I. Modulation of Neisseria gonorrhoeae susceptibility to vertebrate antibacterial peptides due to a member of the resistance/nodulation/division efflux pump family. Proc. Natl. Acad. Sci. USA. 95:1998;1829-1833.
23. Amsterdam D. Susceptibility testing of antimicrobials in liquid media. Loman V. Antibiotics in Laboratory Medicine. 4th Ed. 1996;52-111 Williams and Wilkins, Baltimore, MD.
24. Deamer D., Bangham A.D. Large volume liposomes by an ether vaporization method. Biochim. Biophys. Acta. 443:1976;629-634.
25. Kang J.H., Shin S.Y., Jang S.Y., Lee M.K., Kahm K.S. Release of aqueous contents from phospholipid vesicles induced by cecropin A (1-8)-magainin 2 (1-12) hybrid and its analogues. J. Pept. Res. 52:1998;45-50.
26. Bangalore N., Travis J., Onunka V.C., Pohl J., Shafer W.M. Identification of the primary antimicrobial domains in human neutrophil cathepsin G. J. Biol. Chem. 265:1990;13584-13588.
27. Miyasaki K., Qu X.-D., Harwig S.S.L., Cho Y., Lehrer R.I. Identification of CG-1, a natural peptide antibiotic derived from human neutrophil cathepsin G. G. Adv. Dent. Res. 9:1995;63-65.