Optimization of direct cell counting in sediment

Journal of Microbiological Methods

Volumn 52, Issue 1, 2003, Pages 39-46

  Gough, Heidi L ^a   Stahl, David A ^b  

^a Northwestern University   (United States)

^b University of Washington   (United States)

Author keywords

Bacteria; Dilution; Direct count; Freshwater; Metal; Sediment

Indexed keywords

FRESH WATER; METAL;

ACCURACY; ARTICLE; BACTERIAL COUNT; CELL COUNT; CONTROLLED STUDY; DILUTION; METHODOLOGY; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SEDIMENT; STATISTICS;

NON-PROGRAMMATIC;

BACTERIA; COLONY COUNT, MICROBIAL; GEOLOGIC SEDIMENTS; STAINING AND LABELING; WATER MICROBIOLOGY;

EID: 0037208468     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00135-5     Document Type: Article

References (22)

1. Bakken L.R. Separation and purification of bacteria from soil. Applied and Environmental Microbiology. 49(6):1985;1482-1487.
2. Böttcher M.E., Hespenheide B., Llobet-Brossa E., Beardsley C., Larsen O., Schramm A., Wieland A., Böttcher G., Berninger U.G., Amann R. The biogeochemistry, stable isotope geochemistry, and microbial community structure of a temperate intertidal mudflat: an integrated study. Continental Shelf Research. 20(12-13):2000;1749-1769.
3. Epstein S.S., Rossel J. Enumeration of sandy sediment bacteria - search for an optimal protocol. Marine Ecology. Progress Series. 117(1-3):1995;289-298.
4. Epstein S.S., Alexander D., Cosman K., Dompe A., Gallagher S., Jarsobski J., Laning E., Martinez R., Panasik G., Peluso C., Runde R., Timmer E. Enumeration of sandy sediment bacteria: are the counts quantitative or relative? Marine Ecology. Progress Series. 151(1-3):1997;11-16.
5. Findlay R.H., King G.M., Watling L. Efficacy of phospholipid analysis in determining microbial biomass in sediments. Applied and Environmental Microbiology. 55(11):1989;2888-2893.
6. Frischer M.E., Danforth J.M., Healy M.A.N., Saunders F.M. Whole-cell versus total RNA extraction for analysis of microbial community structure with 16S rRNA-targeted oligonucleotide probes in salt marsh sediments. Applied and Environmental Microbiology. 66(7):2000;3037-3043.
7. Furtado A.L.D., Casper P. Different methods for extracting bacteria from freshwater sediment and a simple method to measure bacterial production in sediment samples. Journal of Microbiological Methods. 41(3):2000;249-257.
8. Jones J.G., Orlandi M.J.L.G., Simon B.M. A microbiological study of sediments from the Cumbrian lakes. Journal of General Microbiology. 115:1979;37-48.
9. Kelly J.J., Tate R.L. Effects of heavy metal contamination and remediation on soil microbial communities in the vicinity of a zinc smelter. Journal of Environmental Quality. 27(3):1998;609-617.
10. Kepner R.L., Pratt J.R. Effects of sediments on estimates of bacterial density. Transactions of the American Microscopical Society. 112(4):1993;316-330.
11. Kepner R.L., Pratt J.R. Use of fluorochromes for direct enumeration of total bacteria in environmental samples - past and present. Microbiological Reviews. 58(4):1994;603-615.
12. Kirchman D., Sigda J., Kapuscinski R., Mitchell R. Statistical analysis of the direct count method for enumerating bacteria. Applied and Environmental Microbiology. 44(2):1982;376-382.
13. Riis V., Lorbeer H., Babel W. Extraction of microorganisms from soil: evaluation of the efficiency by counting methods and activity measurements. Soil Biology and Biochemistry. 30(12):1998;1573-1581.
14. Rublee P., Dornseif B.E. Direct counts of bacteria in sediments of a North Carolina salt marsh. Estuaries. 1(3):1978;188-191.
15. Schallenberg M., Kalff J. The ecology of sediment bacteria in lakes and comparisons with other aquatic ecosystems. Ecology. 74(3):1993;919-934.
16. Schallenberg M., Kalff J., Rasmussen J.B. Solutions to problems in enumerating sediment bacteria by direct counts. Applied and Environmental Microbiology. 55(5):1989;1214-1219.
17. Tao S.F., Taghon G.L. Enumeration of protozoa and bacteria in muddy sediment. Microbial Ecology. 33(2):1997;144-148.
18. Velji M.I., Albright L.J. Microscopic enumeration of attached marine-bacteria of seawater, marine sediment, fecal matter, and kelp blade samples following pyrophosphate and ultrasound treatments. Canadian Journal of Microbiology. 32(2):1986;121-126.
19. Velji M.I., Albright L.J. Improved sample preparation for enumeration of aggregated aquatic substrate bacteria. Kemp P.F., Sherr B.F., Sherr E.B., Cole J.J. Handbook of Methods in Aquatic Microbial Ecology. 1993;139-142 Lewis Publishers, Boca Raton, FL.
20. Wardlaw A.C. Practical Statistics for Experimental Biologists. 1985;Wiley, New York.
21. Webb S.M., Leppard G.G., Gaillard J.F. Zinc speciation in a contaminated aquatic environment: characterization of environmental particles by analytical electron microscopy. Environmental Science and Technology. 34(10):2000;1926-1933.
22. Zar J.H. Biostatistical Analysis. 1984;Prentice-Hall, Englewood Cliffs, NJ.