Whole-cell versus total RNA extraction for analysis of microbial community structure with 16s rRNA-targeted oligonucleotide probes in salt marsh sediments

Applied and Environmental Microbiology

Volumn 66, Issue 7, 2000, Pages 3037-3043

  Frischer, Marc E ^a   Danforth, Jean M ^a   Newton Healy, Michele A ^a,b   Saunders, F Michael ^b  

^a SKIDAWAY INSTITUTE OF OCEANOGRAPHY   (United States)

^b Georgia Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; CELL EXTRACT; RNA 16S;

COMMUNITY STRUCTURE; EXTRACTION METHOD; MICROBIAL COMMUNITY; RNA; SALTMARSH; SEDIMENT;

ANALYTIC METHOD; ARTICLE; BACTERIAL MEMBRANE; BACTERIUM COLONY; BACTERIUM DETECTION; CELL COUNT; CELL HYBRIDIZATION; INTERMETHOD COMPARISON; NONHUMAN; OLIGONUCLEOTIDE PROBE; RNA ANALYSIS; SEDIMENT; SULFATE REDUCING BACTERIUM;

ECOSYSTEM; GEOLOGIC SEDIMENTS; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; PHOSPHORUS; PHYLOGENY; RADIOACTIVE TRACERS; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SULFUR-REDUCING BACTERIA; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS); DELTAPROTEOBACTERIA; NEGIBACTERIA; PROKARYOTA; PROTEOBACTERIA;

EID: 0033943880     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.7.3037-3043.2000     Document Type: Article

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