Development and laboratory evaluation of a new recombinant ELISA for the serodiagnosis of Lyme disease

Wiener Klinische Wochenschrift

Volumn 114, Issue 13-14, 2002, Pages 580-585

  Hunfeld, Klaus Peter ^a   Ernst, Manfred ^a   Zachary, Pierre ^a   Jaulhac, Benoit ^a   Sonneborn, Hans Hermann ^a   Brade, Volker ^a  

^a UNIVERSITY HOSPITAL FRANKFURT   (Germany)

Author keywords

Borrelia burgdorferi; ELISA; Lyme disease; Recombinant proteins; Serodiagnosis

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; RHEUMATOID FACTOR; BACTERIAL PROTEIN; BACTERIUM ANTIBODY; DIAGNOSTIC AGENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; RECOMBINANT PROTEIN;

ANTIBODY DETECTION; ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL STRAIN; BORRELIA AFZELII; BORRELIA BURGDORFERI; CELL LYSATE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC APPROACH ROUTE; DIAGNOSTIC VALUE; DISEASE SEVERITY; ENZYME LINKED IMMUNOSORBENT ASSAY; HERPES SIMPLEX VIRUS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; LABORATORY DIAGNOSIS; LEPTOSPIROSIS; LYME DISEASE; MAJOR CLINICAL STUDY; SERODIAGNOSIS; TOXOPLASMOSIS; ADULT; BLOOD; COMPARATIVE STUDY; CROSS REACTION; DIFFERENTIAL DIAGNOSIS; ERYTHEMA CHRONICUM MIGRANS; EVALUATION; FEMALE; IMMUNOLOGY; MALE; METHODOLOGY; SENSITIVITY AND SPECIFICITY; SEROLOGY;

ADULT; ANTIBODIES, BACTERIAL; BACTERIAL PROTEINS; BORRELIA BURGDORFERI GROUP; COMPARATIVE STUDY; CROSS REACTIONS; DIAGNOSIS, DIFFERENTIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHEMA CHRONICUM MIGRANS; FEMALE; HUMAN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LYME DISEASE; MALE; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; HUMANS;

EID: 0037205673     PISSN: 00435325     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Lyme disease
2. Interlaboratory comparison of test results for detection of Lyme disease by 516 participants in the Wisconsin State Laboratory of Hygiene/College of American Pathologists proficiency testing program
3. The overdiagnosis of Lyme disease
4. Quality of Lyme disease serology. Lessons from the German Proficiency Testing Program 1999-2001
5. Sensitive and specific serodiagnosis of Lyme disease by ELISA with a peptide based on an immunodomminant conserved region of Borrelia burgdorferi VlSE
6. Recombinant chimeric Borrelia proteins for diagnosis of Lyme disease
7. Peptide-based OspC ELISA for serodiagnosis of Lyme borreliosis
8. Serologic diagnosis of Lyme borreliosis by ELISAs with recombinant antigens
9. ELISAs with recombinant flagellin fragments derived from different species of Borrelia burgdorferi sensu lato for the serodiagnosis of Lyme neuroborreliosis
10. Recombinant outer surface protein C ELISA for the diagnosis of early Lyme disease
11. Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease
12. Qualitätsstandards in der mikrobiologisch-infektiologischen Diagnostik
13. Serologically proven double infection with TBEV and Borrelia burgdorferi
14. Laboratory diagnosis of Lyme disease
15. Cross-reactive proteins of Borrelia burgdorferi
16. Immunochemical characterisation of and isolation of the gene for a Borrelia burgdorferi 60-kilodalton antigen common to a wide range of bacteria
17. Mapping the major antigenic domaines of the native flagellar antigen of Borrelia burgdorferi
18. Diagnostics
19. Analysis of the intrathecal immune response in neuroborreliosis to a sonicate extract antigen and three recombinant antigens of Borrelia burgdorferi sensu stricto
20. Development of ELISAs using recombinant borrelial antigens for serodiagnosis of Borrelia burgdorferi infection
21. ELISAs with recombinant internal flagellin fragments derived from different species of Borrelia burgdorferi sensu lato for the serodiagnosis of Lyme neuroborreliosis
22. Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis
23. Immunoblot using recombinant antigens derived from different genospecies of Borrelia burgdorferi sensu lato
24. Impact of strain heterogeneity on Lyme disease serology in Europe: Comparison of ELISAs using different species of Borrelia burgdorferi sensu lato
25. Diagnostic value of proteins of three Borrelia species (Borrelia burgdorferi sensu lato) and implications for developement and use of recombinant antigens for serodiagnosis of Lyme borreliosis in Europe