Double round hybridization of membrane based cDNA arrays: Improved background reduction and data replication

Journal of Neuroscience Methods

Volumn 118, Issue 1, 2002, Pages 59-62

  Donovan, David M ^a   Becker, Kevin G ^a  

^a NATIONAL INSTITUTE ON AGING   (United States)

Author keywords

Background; cDNA; Gene expression; Hybridizations; Microarray; Neuronal; Probe; Radioactive

Indexed keywords

COMPLEMENTARY DNA; GLASS; NYLON; PHOSPHORUS 32;

ARTICLE; CONTROLLED STUDY; COST; DNA MICROARRAY; FILTER; GENE EXPRESSION; HYBRIDIZATION; MEMBRANE; NERVE CELL; PRIORITY JOURNAL; RADIOACTIVITY; REPRODUCIBILITY; TECHNIQUE; GENETICS; INSTRUMENTATION; METABOLISM; METHODOLOGY; STANDARD; STATISTICS;

DNA, COMPLEMENTARY; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REPRODUCIBILITY OF RESULTS; STATISTICS;

EID: 0037199260     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00141-3     Document Type: Article

References (8)

1. Assembly and use of a broadly applicable neural cDNA microarray
2. A murine dopamine neuron-specific cDNA library and microarray: increased COXI expression during methamphetamine neurotoxicity
3. Delineation of prognostic biomarkers in prostate cancer
4. The role of tissue microdissection in cancer research
5. Expression profile of transcripts in Alzheimer's disease tangle-bearing CA1 neurons
6. Importance of replication in microarray gene expression studies: statistical methods and evidence from repetitive cDNA hybridizations
7. Genome-wide expression profiling of mid-gestation placenta and embryo using a 15 000 mouse developmental cDNA microarray
8. Radioactive 33-P probes in hybridization to glass cDNA microarrays using neural tissues