Expression of a functional human interleukin-18 in yeast

Enzyme and Microbial Technology

Volumn 30, Issue 6, 2002, Pages 703-709

  Lim, Young Yi ^a   Lee, Mi Yae ^a   Chung, Bong Woo ^a   Park, Seung Moon ^a   Park, Sung Goo ^b   Jang, Yong Suk ^a   Yang, Moon Sik ^a   Kim, Dae Hyuk ^a  

^a Chonbuk National University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Human interleukin 18; Saccharomyces cerevisiae

Indexed keywords

ALCOHOLS; CLONING; CROPS; DNA; ESCHERICHIA COLI; GENES; MOLECULAR WEIGHT; PHOSPHATES; PROTEINS; YEAST;

NORTHERN BLOT ANALYSIS;

ENZYMES;

ALCOHOL DEHYDROGENASE; AMYLASE; COMPLEMENTARY DNA; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; RECOMBINANT INTERLEUKIN 18;

AMINO ACID SEQUENCE; BIOASSAY; CELL GROWTH; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; CYTOKINE PRODUCTION; CYTOKINE RELEASE; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037182308     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00043-1     Document Type: Conference Paper

References (32)

1. Cloning of a new cytokine that induce IFN-γ production by T cells
2. Cloning of the cDNA for human IFN-γ-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein
3. Interleukin-18, and interleukin-1β: Two cytokine substrates for ICE (caspase-1)
4. Interleukin-18
5. Production of a biologically active human interleukin 18 requires its prior synthesis as pro-IL-18
6. Establishment of the transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony stimulating factor
7. Expression of murine GM-CSF from recombinant Aspergillus niger
8. Alpha-factor-directed synthesis, and secretion of mature foreign proteins in Saccharomyces cerevisiae
9. Enhanced iron uptake of Saccharomyces cerevisiae by the heterologous expression of a tadpole ferritin gene
10. Expression of glucose oxidase by using recombinant yeast
11. Expression and secretion of rice-alpha-amylase by Sacchromyces cerevisiae
12. Enhanced and targeted expression of fungal phytase in Sacchromyces cerevisiae
13. Simultaneous introduction of multiple mutations using overlap extension PCR
14. Transformation of intact yeast cells treated with alkali cations
15. Genomic sequencing
16. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
17. Concentration of proteins and removal of solutes
18. Optimizing hydrolysis of N-linked high-mannose oligosaccharides by endo-β-N-acetylglucosaminidase H
19. A rapid, and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding
20. A simple, and sensitive bioassay for the detection of human interleukin-18/interferon-gamma-inducing factor using human myelomonocytic KG-1 cells
21. Sequence from picomole quanities of proteins electroblotted onto polyvinylidene difluoride membranes
22. High level of expression of a protective antigen of schistosomes in Saccharomyces cerevisiae
23. Translation and stability of an Escherichia coli beta-galactosidase mRNA expressed under the control of pyruvate kinase sequences in Saccharomyces cerevisiae
24. Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter
25. Transcriptional regulation of the Bacillus subtilis gluticol dehydrogenase gene
26. Activation of interferon-γ inducing factor mediated by interleukin-1β converting enzyme
27. Secretion of N-glycosylated human recombinant interleukin-1 beta in Saccharomyces cerevisiae
28. Secretion of N-glycosylated human recombinant interleukin-1 beta in Saccharomyces cerevisiae using a leader peptide from Candida albicans. Effect of N-linked glycosylation on biological activity
29. Lymphokines and cytokines as cancer treatment. Immunotherapy realized
30. Lessons from the clinical trails of interleukin-2
31. Treatment of 283 consecutive patients with metastatic melanoma or renal cell cancer using high-dose bolus interleukin 2