An ion conductor that recognizes osmotically-stressed phospholipid bilayers

Journal of the American Chemical Society

Volumn 124, Issue 38, 2002, Pages 11254-11255

  Bandyopadhyay, Prasun ^a   Bandyopadhyay, Punam ^a   Regen, Steven L ^a  

^a LEHIGH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; CONDUCTOR; EQUILIBRIUM CONSTANT; ION TRANSPORT; MOLECULAR RECOGNITION; OSMOLARITY; OSMOSIS; PHASE TRANSITION; PHOSPHOLIPID BILAYER; TRANSPORT KINETICS;

ANTIPORTERS; LIPID BILAYERS; LIPOSOMES; OSMOLAR CONCENTRATION; PHOSPHOLIPIDS; PHOSPHORYLCHOLINE;

EID: 0037174387     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja026895s     Document Type: Article

References (9)

1. Bandyopadhyay, P.; Janout, V.; Zhang, L.; Regen, S. L. J. Am. Chem. Soc. 2001, 123, 7691.
2. (a) Bummer, P. M.; Zografi, G. J. Pharm. Sci. 1988, 77, 1021.
3. (b) Naka, K.; Sadownik, A.; Regen, S. L. J. Am. Chem. Soc. 1993, 115, 2278.
4. + is added, externally; i.e., 1 is not membrane-disrupting.
5. Benachir, T.; Lafleur, M. Biophys. J. 1996, 70, 831.
6. Pregel, M. J.; Jullien, L.; Lehn, J. M. Angew. Chem., Int. Ed. Engl. 1992, 31, 1637.
7. Otto, S.; Osifchin, M.; Regen, S. L. J. Am. Chem. Soc. 1999, 121, 7276.
8. A similar experiment that was carried out using 1 mol % of 1 and a 10-atm osmotic gradient, with lower salt concentrations (the internal NaCl and external LiCl concentrations were reduced from 450 and 208 mM to 250 and 49 mM, respectively) gave the same rate constant, within experimental error (±10%). Thus, ionic strength is not a significant factor in these experiments.
9. Hypotonicity produces osmotic pressures of ca. 15-20 and 0.9-5 atm in Gram positive and Gram negative bacteria, respectively. In contrast, mammalian membranes are relatively stress-free: Csonka, L. N. Microbiol. Rev. 1989, 53, 121.